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The human blood-brain barrier (BBB) is a complex multi-dimensional reticular barrier system composed of cerebral microvascular endothelial cells, pericytes, astrocytes and a variety of neurons. The conventional in vitro cell culture model fails to truly present the dynamic hemodynamics of BBB and the interaction between neurons. And it is even more impossible to explore brain-related multi-organ diseases, which brings huge obstacles to explore diseases of the central nervous system and the interaction between brain-related multi-organs, and evaluate drug efficacy. Miniaturized microfluidics based BBB chips are being commonly used to co-culture a variety of cells on a small-sized chip to construct a three-dimensional (3D) BBB or BBB-related organ disease models. By combining with other electrophysiological, biochemical sensors or equipment and imaging systems, it can in real time and quickly screen disease-related markers and evaluate drug efficacy. This review systematically summarized the research progress of in vitro BBB and BBB-related organ chips, and analyzed the obstacles of BBB models in depth. Parallelly combined with the current research trends and hot spots, we give the further improvement measures of microfluidic BBB chips., (Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.)

Tangchong (ཐང་ཕྲོམ།), a term in the Tibetan language, encompasses a diverse group of plants belonging to different genera of the Solanaceae family. These plants have been utilized in traditional Tibetan medicine for centuries and are currently still employed to treat a variety of ailments, including acute and severe abdominal pain, intestinal obstruction, epilepsy, ascariasis, lung abscess, and other diseases. The therapeutic properties of tangchong are attributed to the presence of tropane alkaloids (TAs), although recent research has also revealed their toxicity. The purpose of this review is to provide a comprehensive analysis of the traditional uses of tangchong, as well as the phytochemical and pharmacological studies conducted on this plant. The review aims to offer a critical update on the current state of knowledge of tangchong and to identify new opportunities for exploring its therapeutic potential. To gather information on tangchong, we conducted a thorough search of several scientific databases, including Google Scholar, Web of Science, Scifinder, Baidu Scholar, PubMed, and CNKI. This review summarizes the traditional usage of 11 plants, 168 chemical components, including alkaloids, flavonoids, steroids, amides, coumarins, etc., describes the pharmacological activities of these medicinal plants and their mechanisms of action, provides an elaboration of toxicology, and provides new plant-derived drugs and herbal preparations from tangchong. Clarity regarding the source of the drug is crucial to ensure the safety and efficacy of clinical applications of Tibetan medicine. In this paper, we provide a comprehensive review of tangchong, including its traditional medicinal uses, chemical composition, pharmacology, toxicology, and quality control measures. Our analysis highlights the significant value of tangchong as an ethnomedicinal resource, with pharmacological activities primarily linked to its alkaloid content. Furthermore, this review serves as a valuable reference for future research on alkaloid-containing ethnomedicines. [Display omitted] • This review provides a systematic and comprehensive summary of the Tibetan medicine "Tangchong" for the first time. • This review will facilitate further research and development of the Tibetan medicine "Tangchong", and provide theoretical references for subsequent researchers. • This review will be helpful for the succession and development of Tibetan medicine. [ABSTRACT FROM AUTHOR]