1. Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery.
- Author
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Omar H, Croissant JG, Alamoudi K, Alsaiari S, Alradwan I, Majrashi MA, Anjum DH, Martins P, Laamarti R, Eppinger J, Moosa B, Almalik A, and Khashab NM
- Subjects
- Fluorescent Dyes administration & dosage, Fluorescent Dyes chemistry, HeLa Cells, Humans, Magnetic Phenomena, Nanoparticles administration & dosage, Nanoparticles chemistry, Porosity, Propylamines administration & dosage, Propylamines chemistry, Drug Delivery Systems, Ferric Compounds administration & dosage, Ferric Compounds chemistry, Ferritins administration & dosage, Ferritins chemistry, Green Fluorescent Proteins administration & dosage, Green Fluorescent Proteins chemistry, Nanocomposites administration & dosage, Nanocomposites chemistry, Silicon Dioxide administration & dosage, Silicon Dioxide chemistry
- Abstract
The delivery of large cargos of diameter above 15nm for biomedical applications has proved challenging since it requires biocompatible, stably-loaded, and biodegradable nanomaterials. In this study, we describe the design of biodegradable silica-iron oxide hybrid nanovectors with large mesopores for large protein delivery in cancer cells. The mesopores of the nanomaterials spanned from 20 to 60nm in diameter and post-functionalization allowed the electrostatic immobilization of large proteins (e.g. mTFP-Ferritin, ~534kDa). Half of the content of the nanovectors was based with iron oxide nanophases which allowed the rapid biodegradation of the carrier in fetal bovine serum and a magnetic responsiveness. The nanovectors released large protein cargos in aqueous solution under acidic pH or magnetic stimuli. The delivery of large proteins was then autonomously achieved in cancer cells via the silica-iron oxide nanovectors, which is thus a promising for biomedical applications., (Copyright © 2016. Published by Elsevier B.V.)
- Published
- 2017
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