344 results on '"Colloids in medicine"'
Search Results
2. Plant and Algal Hydrogels for Drug Delivery and Regenerative Medicine
- Author
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Tapan Kumar Giri, Bijaya Ghosh, Tapan Kumar Giri, and Bijaya Ghosh
- Subjects
- Colloids in medicine, Regenerative medicine, Drug delivery systems, Colloi¨des en me´decine, Me´decine re´ge´ne´ratrice, Syste`mes d'administration de me´dicaments
- Abstract
Plant and Algal Hydrogels for Drug Delivery and Regenerative Medicine offers a materials-focused and systematic overview of biopolymeric hydrogels utilized for biomedical applications. The book details the synthesis and characterization of plant and algal-based hydrogels, with each chapter addressing a separate polysaccharide hydrogel type. Specific applications in drug delivery and regenerative medicine are also discussed, highlighting the efficacy, biocompatibility, benefits and challenges for each polysaccharide hydrogel subtype. There is increasing demand for biomaterials which reduce/prevent the host response, inflammation and rejection, hence this book provides a timely resource. Biopolymeric hydrogels have skyrocketed because of their necessity in in vivo applications. They create an environment similar to living tissue, which is both biocompatible and biodegradable. Plant and algal polysaccharides in particular are well-equipped with functional groups that are easily modified for beneficial results. Systematically covers each plant and algal polysaccharide hydrogel subtype, from starch-based hydrogels to pectin and alginate-based hydrogels Provides an end-to-end description of the synthesis, characterization and application of biopolymeric hydrogels for drug delivery and regenerative medicine Appeals to a diverse readership, including those in biomedicine, pharmacy, polymer chemistry, biochemistry, materials science, biomedical engineering, and other biotechnology related disciplines
- Published
- 2021
3. Drug Targeting by Magnetically Responsive Colloids (K)
- Author
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Arias, José L. and Arias, José L.
- Subjects
- Magnetotherapy, Colloids, Nanoparticles--Therapeutic use, Drug delivery systems, Colloids in medicine, Magnetics
- Abstract
Magnetic colloids are very promising stimuli-sensitive materials for drug delivery because of their magnetic field responsiveness. An applied magnetic field can concentrate the magnetic nanodevice at the desired site, keeping it there until the drug is totally released. Furthermore, the versatility of magnetic colloids allows the design of multifunctional nanoplatforms with combined therapy and imaging functionalities against diseases with high morbidity. This new book summarizes the most important advances in the formulation and functionalization of such magnetic micro- and nano-particles, and also describes the recent advances on their use in biomedicine.
- Published
- 2010
4. FORMULATION AND EVALUATION OF GASTRORETENTIVE FLOATING IN-SITU GEL OF MINOCYCLINE HCL.
- Author
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Mercy, Macwan and Banwait, Hardeepsingh
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MINOCYCLINE , *ULCER treatment , *COLLOIDS in medicine , *DRUG delivery systems , *ANTIULCER drugs , *PEPTIC ulcer - Abstract
Peptic ulcer like condition requires delivery of anti-ulcer medicament locally in the stomach. Pediatric and geriatric convenience by ease of administration with enhanced gastric residence time achieved by formulating floating in situ gelling system. Using ionic cross linking method floating in situ gelling system of Minocycline HCl is prepared. Trials batches were prepared to optimize the concentration of sodium alginate, calcium carbonate and various grades of HPMC Prepared formulations were evaluated for viscosity, gel strength, pH, drug content, in vitro buoyancy and in vitro drug release. A 3²factorial design was applied to check effect of varying concentration of sodium alginate (X1) and HPMC K100M CR (X2) on the dependent variables such as viscosity, gelling strength, time required for complete release and floating lag time. Kinetic of drug release, regression analysis and analysis of variance were performed. Trial batch T1 to T4 shows optimum range of sodium alginate, T5 to T7 optimizes the concentration of calcium carbonate and batch B1 to B12 shows that HPMC K100 M CR is having maximum sustaining ability besides uniform raft formation. Formulation F8 was found to be optimum with sodium alginate (2%w/v) and HPMC K100M CR (1.5%w/v), shows release up to 12 hours. Floating in situ gelling system was successfully formulated using sodium alginate as gelling polymer and HPMC K100M CR as a release retardant polymer. [ABSTRACT FROM AUTHOR]
- Published
- 2018
5. Granulated colloidal silicon dioxide-based self-microemulsifying tablets, as a versatile approach in enhancement of solubility and therapeutic potential of anti-diabetic agent: formulation design and in vitro / in vivo evaluation.
- Author
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Pandey, Vikas, Gilhotra, Ritu M., and Kohli, Seema
- Subjects
COLLOIDS in medicine ,SILICA ,DRUG delivery systems ,DRUG bioavailability ,PROPYLENE glycols ,X-ray diffraction - Abstract
The current research work was executed with an aim to explore and promote the potential of self-microemusifying drug delivery systems (SMEDDS) in the form of tablets, in order to enhance solubility and oral bioavailability of poorly aqueous soluble drug Repaglinide (RPG). RPG-loaded liquid SMEDDS were developed consisting Labrafil M 1944CS, Kolliphor EL and Propylene glycol, which were then characterized on various parameters. After characterization and optimization, liquid SMEDDS were converted into solid form by adsorbing on Aeroperl® 300 pharma and polyplasdoneTMXL. Further, selection of suitable excipients was done and mixed with prepared solidified SMEDDS powder followed by the preparation of self-microemulsifying tablets (SMET’s) wet granulation–compression method. SMET’s were subjected to differential scanning calorimetry (DSC) and particle X-ray diffraction (RXRD) studies, results of which indicated transformation of crystalline structure of RPG because of dispersion of RPG at molecular level in liquid SMEDDS. This was further assured by micrographs obtained from scanning electron microscope. SMET’s shown more than 85% (30 min) ofin vitrodrug release in contrast to conventional marketed tablets (13.2%) and pure RPG drug (3.2%). Results ofin vivostudies furnished that SMET’s had shown marked decrease in the blood glucose level and prolonged duration of action (up to 8 h) in comparison with conventional marketed tablets and pure RPG drug. In conclusion, SMET’s serves as a promising tool for successful oral delivery of poorly aqueous soluble drug(s) such as RPG. [ABSTRACT FROM AUTHOR]
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- 2017
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6. An assessment of the ability of the obstruction-scaling model to estimate solute diffusion coefficients in hydrogels.
- Author
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Hadjiev, Nicholas A. and Amsden, Brian G.
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COLLOIDS in medicine , *DRUG design , *DRUG delivery systems , *TISSUE engineering , *SODIUM alginate , *DIFFUSION coefficients - Abstract
The ability to estimate the diffusion coefficient of a solute within hydrogels has important application in the design and analysis of hydrogels used in drug delivery, tissue engineering, and regenerative medicine. A number of mathematical models have been derived for this purpose; however, they often rely on fitted parameters and so have limited predictive capability. Herein we assess the ability of the obstruction-scaling model to provide reasonable estimates of solute diffusion coefficients within hydrogels, as well as the assumption that a hydrogel can be represented as an entangled polymer solution of an equivalent concentration. Fluorescein isothiocyanate dextran solutes were loaded into sodium alginate solutions as well as hydrogels of different polymer volume fractions formed from photoinitiated cross-linking of methacrylate sodium alginate. The tracer diffusion coefficients of these solutes were measured using fluorescence recovery after photobleaching (FRAP). The measured diffusion coefficients were then compared to the values predicted by the obstruction-scaling model. The model predictions were within ± 15% of the measured values, suggesting that the model can provide useful estimates of solute diffusion coefficients within hydrogels and solutions. Moreover, solutes diffusing in both sodium alginate solutions and hydrogels were demonstrated to experience the same degree of solute mobility restriction given the same effective polymer concentration, supporting the assumption that a hydrogel can be represented as an entangled polymer solution of equivalent concentration. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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7. Nanocrystals for enhancement of oral bioavailability of poorly water-soluble drugs.
- Author
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Junyaprasert, Varaporn Buraphacheep and Morakul, Boontida
- Subjects
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NANOCRYSTALS , *ORAL drug administration , *DRUG bioavailability , *COLLOIDS in medicine , *DRUG solubility , *DRUG administration , *DRUG delivery systems , *CELL membranes - Abstract
Nanocrystals, a carrier-free colloidal delivery system in nano-sized range, is an interesting approach for poorly soluble drugs. Nanocrystals provide special features including enhancement of saturation solubility, dissolution velocity and adhesiveness to surface/cell membranes. Several strategies are applied for nanocrystals production including precipitation, milling, high pressure homogenization and combination methods such as Nano- Edge™, SmartCrystal and Precipitation-lyophilization-homogenization (PLH) technology. For oral administration, many publications reported useful advantages of nanocrystals to improve in vivo performances i.e. pharmacokinetics, pharmacodynamics, safety and targeted delivery which were discussed in this review. Additionally, transformation of nanocrystals to final formulations and future trends of nanocrystals were also described. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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8. Visible-light-induced synthesis of pH-responsive composite hydrogels for controlled delivery of the anticonvulsant drug pregabalin.
- Author
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Cevik, Ozlem, Gidon, Dogan, and Kizilel, Seda
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PREGABALIN ,ANTICONVULSANTS ,COLLOIDS in medicine ,CONTROLLED release drugs ,DRUG delivery systems ,COPOLYMERIZATION ,BIOCOMPATIBILITY ,CROSSLINKING (Polymerization) ,THERAPEUTICS - Abstract
We report here a novel method for the synthesis of a pH-responsive composite using visible light. Formation of the pH-responsive layer is based on poly(methacrylic acid- g -ethylene glycol) as the macromer, eosin Y as the photoinitiator and triethanolamine as the co-initiator. The hydrogel was functionalized with hydrophobic domains through incorporation of crosslinked styrene–butadiene–styrene (SBS) copolymer into the pH-responsive prepolymer. Swelling ratios were decreased with the addition of SBS, and resulted in high hydrogel crosslink density. The composite allowed for controlled release of an anticonvulsant model drug, pregabalin, under neutral pH condition and the release was analyzed to describe the mode of transport through the network. In vitro human fibroblast survival assay and in vivo rabbit implantation experiments demonstrated that this hybrid network is not toxic and has desirable biocompatibility properties. This is the first report about the synthesis of a pH-responsive network incorporating crosslinked SBS synthesized under visible light. The approach for multifunctional membranes could allow the incorporation of molecules with specific functionalities so that sequential molecule delivery in response to specific stimuli could be achieved. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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9. Ionically cross-linkable hyaluronate-based hydrogels for injectable cell delivery.
- Author
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Park, Honghyun, Woo, Eun Kyung, and Lee, Kuen Yong
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THERAPEUTIC use of hyaluronic acid , *COLLOIDS in medicine , *BIOMARKERS , *GENE expression , *DRUG delivery systems , *IMMUNOHISTOCHEMISTRY , *MEDICAL research - Abstract
Although hyaluronate is an attractive biomaterial for many biomedical applications, hyaluronate hydrogels are generally formed using chemical cross-linking reagents that may cause unwanted side effects, including toxicity. We thus propose to design and prepare ionically cross-linkable hyaluronate compounds that can form gels in the presence of counter-ions. This study is based on the hypothesis that introduction of alginate to hyaluronate backbones (hyaluronate- g -alginate) could allow for gel formation in the presence of calcium ions. Here, we demonstrated ease of formation of cross-linked structures with calcium ions without additional chemical cross-linking reagents in hyaluronate- g -alginate (HGA) gels. The mechanical properties of HGA gels were regulated through changes in polymer composition and calcium concentration. We also confirmed that HGA gels could be useful in regenerating cartilage in a mouse model following subcutaneous injection into the dorsal region with primary chondrocytes. This finding was supported by histological and immunohistochemical analyses, glycosaminoglycan quantification and chondrogenic marker gene expression. This approach to the design and tailoring of ionically cross-linkable biomedical polymers may be broadly applicable to the development of biomaterials, especially in the drug delivery and tissue engineering fields. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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10. Mechanistic studies of an autonomously pulsing hydrogel/enzyme system for rhythmic hormone delivery.
- Author
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Bhalla, Amardeep S. and Siegel, Ronald A.
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LUTEINIZING hormone releasing hormone receptors , *HORMONE therapy , *DRUG delivery systems , *MEMBRANE permeability (Biology) , *COLLOIDS in medicine , *DRUG design - Abstract
Numerous hormones are known to be endogenously secreted in a pulsatile manner. In particular, gonadotropin replacing hormone (GnRH) is released in rhythmic pulses, and disruption of this rhythm is associated with pathologies of reproduction and sexual development. In an effort to develop an implantable, rhythmic delivery system, a scheme has been demonstrated involving a negative feedback instability between a pH-sensitive membrane and enzymes that convert endogenous glucose to hydrogen ion. A bench prototype system based on this scheme was previously shown to produce near rhythmic oscillations in internal pH and in GnRH delivery over a period of one week. In the present work, a systematic study of conditions permitting such oscillations is presented, along with a study of factors causing period of oscillations to increase with time and ultimately cease. Membrane composition, glucose concentration, and surface area of marble (CaCO 3 ), which is incorporated as a reactant, were found to affect the capacity of the system to oscillate, and the pH range over which oscillations occur. Accumulation of gluconate − and Ca 2 + in the system over time correlated with lengthening of oscillation period, and possibly with cessation of oscillations. Enzyme degradation may also be a factor. These studies provide the groundwork for future improvements in device design. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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11. Development and in vitro assessment of enzymatically-responsive poly(ethylene glycol) hydrogels for the delivery of therapeutic peptides.
- Author
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Van Hove, Amy H., G. Beltejar, Michael-John, and Benoit, Danielle S.W.
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COLLOIDS in medicine , *DRUG development , *DRUG delivery systems , *PEPTIDES , *CONTROLLED release drugs , *POLYETHYLENE glycol , *MATRIX metalloproteinases , *IN vitro studies , *THERAPEUTICS - Abstract
Despite the recent expansion of peptide drugs, delivery remains a challenge due to poor localization and rapid clearance. Therefore, a hydrogel-based platform technology was developed to control and sustain peptide drug release via matrix metalloproteinase (MMP) activity. Specifically, hydrogels were composed of poly(ethylene glycol) and peptide drugs flanked by MMP substrates and terminal cysteine residues as crosslinkers. First, peptide drug bioactivity was investigated in expected released forms (e.g., with MMP substrate residues) in vitro prior to incorporation into hydrogels. Three peptides (Qk (from Vascular Endothelial Growth Factor), SPARC 113 , and SPARC 118 (from Secreted Protein Acidic and Rich in Cysteine)) retained bioactivity and were used as hydrogel crosslinkers in full MMP degradable forms. Upon treatment with MMP2, hydrogels containing Qk, SPARC 113 , and SPARC 118 degraded in 6.7, 6, and 1 days, and released 5, 8, and, 19% of peptide, respectively. Further investigation revealed peptide drug size controlled hydrogel swelling and degradation rate, while hydrophobicity impacted peptide release. Additionally, Qk, SPARC 113 , and SPARC 118 releasing hydrogels increased endothelial cell tube formation 3.1, 1.7, and 2.8-fold, respectively. While pro-angiogenic peptides were the focus of this study, the design parameters detailed allow for adaptation of hydrogels to control peptide release for a variety of therapeutic applications. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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12. Advances in hydrogel delivery systems for tissue regeneration.
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Toh, Wei Seong and Loh, Xian Jun
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COLLOIDS in medicine , *REGENERATION (Biology) , *DRUG delivery systems , *POLYMERIZATION , *EXTRACELLULAR matrix , *REGENERATIVE medicine , *CELL differentiation - Abstract
Hydrogels are natural or synthetic polymer networks that have high water-absorbing capacity and closely mimic native extracellular matrices. As hydrogel-based cell delivery systems are being increasingly employed in regenerative medicine, several advances have been made in the hydrogel chemistry and modification for enhanced control of cell fate and functions, and modulation of cell and tissue responses against oxidative stress and inflammation in the tissue environment. This review aims to provide the state-of-the-art overview of the recent advances in field, discusses new perspectives and challenges in the regeneration of specific tissues, and highlights some of the limitations of current systems for possible future advancements. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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13. Synthesis of attapulgite/N-isopropylacrylamide and its use in drug release.
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Li, Xiaomo, Zhong, Hui, Li, Xiaorong, Jia, Feifei, Cheng, Zhipeng, Zhang, Lili, Yin, Jingzhou, An, Litao, and Guo, Liping
- Subjects
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PHYSIOLOGICAL effects of acrylamide , *CONTROLLED release drugs , *COLLOIDS in medicine , *DRUG delivery systems , *DRUG synthesis , *FULLER'S earth - Abstract
Environmentally sensitive hydrogels as one of the most potential drug delivery systems have gained considerable interest in recent years. In the present study, we synthesized a newly temperature-responsive composite hydrogel based on attapulgite (ATP) and poly (N-isopropylacrylamide) (PNIPAM) as the localized drug carriers for drug delivery. The as-prepared ATP/PNIPAM hydrogel has large aperture which significantly improved the quantity of adsorption of drugs, exhibiting the excellent properties of drug release. The scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) were used to characterize the ATP/PNIPAM. The swelling/deswelling behaviors and the release of ciprofloxacin lactate were studied. When the temperature was below the low critical solution temperature (LCST), the swelling property of hydrogels was excellent and the swelling rate was large. And, the drug release rate increased with the increase of the content of attapulgite in the composite hydrogel when it was put in the buffer solution (pH 7.38) at 37.0 °C. Therefore, the composite hydrogels might be very useful for its application in biomedical fields. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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14. Targeting of Intravenous Polymeric Nanoparticles by Differential Protein Adsorption.
- Author
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Staufenbiel, Sven, Weise, Christoph, and Müller, Rainer H.
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INTRAVENOUS injections , *BIOPOLYMERS , *NANOPARTICLES , *BLOOD proteins , *ADSORPTION (Biology) , *COLLOIDS in medicine , *POLYMERIC drug delivery systems - Abstract
The organ distribution of intravenously injected nanoparticles is determined by the composition of the blood protein adsorption pattern occurring after injection. This is exploited in the concept of 'differential protein adsorption' for drug targeting, which is briefly discussed. The surface properties of the nanoparticles determine the adsorption patterns, by controlling the surface properties one can generate adsorption patterns required for achieving the desired organ distribution. The efficiency of this principle is shown by reviewing different organ distributions achieved using various polymeric nanoparticles with different surface properties. Surface modification can be obtained by polymer adsorption and can create nanoparticles circulating in the blood, or accumulating in targets such as bone marrow and brain. The protein adsorption patterns were analyzed using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Here, adsorption patterns of dendritic polymer nanoparticles were investigated, where the used polymer was dendritic polyglycerol sulfate. They showed reduced opsonization and preferential adsorption of apolipoprotein A-I with brain targeting potential. In perspective, the principle of surface property modification by polymer/stabilizer adsorption can be transferred to intravenous drug nanocrystals. A hybrid system of nanocrystal and polymeric nanoparticles is suggested, the polymeric nanoparticle with nanocrystal core. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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15. Controlled release of sphingosine-1-phosphate agonist with gelatin hydrogels for macrophage recruitment.
- Author
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Murakami, Masahiro, Saito, Takashi, and Tabata, Yasuhiko
- Subjects
SPHINGOSINE-1-phosphate ,GELATIN ,MACROPHAGES ,COLLOIDS in medicine ,DRUG delivery systems - Abstract
The objective of this study is to design a drug delivery system (DDS) for the in vivo promotion of macrophage recruitment. As the drug, a water-insoluble agonist of sphingosine-1-phosphate type 1 receptor (SEW2871) was selected. SEW2871 (SEW) was water-solubilized by micelle formation with gelatin grafted by l -lactic acid oligomer. SEW micelles were mixed with gelatin, followed by dehydrothermal crosslinking of gelatin to obtain gelatin hydrogels incorporating SEW micelles. SEW was released from the hydrogels incorporating SEW micelles in vitro and in vivo. The water-solubilized SEW showed in vitro macrophage migration activity. When implanted into the back subcutis or the skin wound defect of mice, the hydrogel incorporating SEW micelles promoted macrophage migration toward the tissue around the implanted site to a significantly great extent compared with SEW-free hydrogel and that mixed with SEW micelles. The hydrogel is a promising DDS to enhance macrophage recruitment in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
16. Evaluation of safety and efficacy as an adjuvant for the chitosan-based vaccine delivery vehicle ViscoGel in a single-blind randomised Phase I/IIa clinical trial.
- Author
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Neimert-Andersson, Theresa, Binnmyr, Jonas, Enoksson, Mattias, Langebäck, Joakim, Zettergren, Louise, Hällgren, Anne-Charlotte, Franzén, Henry, Lind Enoksson, Sara, Lafolie, Pierre, Lindberg, Alf, Al-Tawil, Nabil, Andersson, Mats, Singer, Peter, Grönlund, Hans, and Gafvelin, Guro
- Subjects
- *
IMMUNOLOGICAL adjuvants , *VACCINE effectiveness , *VACCINE safety , *COLLOIDS in medicine , *DRUG delivery systems , *HUMORAL immunity , *CHITOSAN , *LABORATORY mice - Abstract
ViscoGel, a chitosan-based hydrogel, has earlier been shown to improve humoral and cell-mediated immune responses in mice. In this study, a Phase I/IIa clinical trial was conducted to primarily evaluate safety and secondarily to study the effects of ViscoGel in combination with a model vaccine, Act-HIB to Haemophilus influenzae type b, administered as a single intramuscular injection. Healthy volunteers of both sexes, ages 22–50 and not previously vaccinated to HIB, were recruited. The trial had two phases. In Phase A, three ascending dose levels of ViscoGel (25, 50 and 75 mg) were evaluated for safety in 3 × 10 subjects. Phase B had a single-blind, randomised, parallel-group design evaluating safety and efficacy in five groups, 20 subjects/group, comparing vaccination with 0.2 μg or 2 μg Act-HIB alone or combined with ViscoGel (50 mg) and one group receiving the standard Act-HIB dose (10 μg). No safety or tolerability concerns were identified. Local, transient reactions at the injection site were the most common adverse events. These were more frequent in groups receiving Act-HIB + ViscoGel, while other AEs were recorded at similar frequency in Act-HIB and Act-HIB + ViscoGel groups. Efficacy was evaluated by measuring serum anti-HIB antibodies and cellular responses in peripheral blood mononuclear cells (PBMC). There was a large variation in baseline anti-HIB antibody titres and no adjuvant effect was observed on the anti-HIB antibody production in groups vaccinated with Act-HIB + ViscoGel. ELISpot analyses revealed increased interferon-γ (IFN-γ) responses to Act-HIB in PBMCs from subjects vaccinated with Act-HIB in combination with ViscoGel, compared to groups receiving Act-HIB alone. Moreover, ViscoGel counteracted an inhibitory effect of Act-HIB vaccination on the IFN-γ response to both the vaccine itself and an irrelevant influenza antigen. In summary, ViscoGel was found to be safe and well-tolerated, supporting further examination of ViscoGel as a new innovative vehicle for vaccine development. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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17. Chitosan/glucose 1-phosphate as new stable in situ forming depot system for controlled drug delivery.
- Author
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Supper, Stephanie, Anton, Nicolas, Boisclair, Julie, Seidel, Nina, Riemenschnitter, Marc, Curdy, Catherine, and Vandamme, Thierry
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CHITOSAN , *GLUCOSE phosphates , *DRUG delivery systems , *BODY temperature , *COLLOIDS in medicine , *DIFFERENTIAL scanning calorimetry , *GELATION - Abstract
Chitosan (CS)-based thermosensitive solutions that turn into semi-solid hydrogels upon injection at body temperature have increasingly drawn attention over the last decades as an attractive new type of in situ forming depot (ISFD) drug delivery system. Despite the great potential of the standard CS/β-glycerophosphate (β-GP) thermogelling solutions, their lack of stability over time at room temperature as well as at refrigerated conditions renders them unsuitable as ready-to-use drug product. In the present study, we investigated Glucose-1-Phosphate (G1-P) as an alternative gelling agent for improving the stability of CS-based ISFD solutions. The in vitro release performance of CS/G1-P formulations was assessed using several model compounds. Furthermore, the local tolerance of subcutaneously implanted CS/G1-P hydrogels was investigated by histological examination over three weeks. The thermogelling potential of CS/G1-P solutions, determined by rheology, is dependent on the polymer molecular weight (Mw) and concentration as well as on the G1-P concentration. Differential scanning calorimetry (DSC) measurements confirmed that sol/gel transition takes place at around body temperature and is not fully thermo-reversible. The long term storage stability was evaluated through the appearance, pH, viscosity and gelation time at 37 °C of the solution. The results emphasized an enhanced stability of the CS/G1-P system compared to the standard CS/β-GP. CS solution with 0.40 mmol/g G1-P is stable for at least 9 months at 2–8 °C, versus less than 1 month when using β-GP as gelling agent. Furthermore, the solution is easy to inject, as evidenced from injectability evaluation using 23–30 G needles. In vitro release experiments showed a sustained release over days to weeks for hydrophilic model compounds, demonstrating thereby that CS/G1-P may be suitable for the prolonged delivery of drugs. The inflammatory reaction observed in the tissue surrounding the hydrogel in rats was a typical foreign body reaction, similar to the one observed for CS/β-GP hydrogels. These features confirm the potential of CS/G1-P solutions as an injectable ready-to-use in situ forming hydrogel. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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18. In situ gelling polyvalerolactone-based thermosensitive hydrogel for sustained drug delivery.
- Author
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Mishra, Gyan P., Kinser, Reid, Wierzbicki, Igor H., Alany, Raid G., and Alani, Adam W.G.
- Subjects
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COLLOIDS in medicine , *DRUG delivery systems , *GEL permeation chromatography , *CRITICAL micelle concentration , *POLYETHYLENE glycol , *DEXAMETHASONE , *BLOCK copolymers - Abstract
Biodegradable poly(ethyleneglycol)–poly(valerolactone)–poly(ethyleneglycol) [PEG–PVL–PEG] copolymers were synthesized through ring opening polymerization of δ-valerolactone (VL) followed by the coupling of monomethoxy poly(ethyleneglycol–poly(valerolactone) (mPEG–PVL) with hexamethylene diisocyanate (HDI). The copolymers were characterized by 1 H NMR, FT-IR, and GPC. Block copolymers of PEG and PVL with different VL/PEG molar ratios were successfully synthesized. One of the copolymers (Copolymer 2, PEG 550 – PVL 6768 – PEG 550 ) displayed a sol–gel transition at a physiological temperature based on the test tube inverting method and rheological studies. The thermogelling copolymer demonstrated a characteristic crystalline peak for PVL block as determined by DSC and XRD analysis. In vitro release from the copolymer hydrogel matrix indicated that dexamethasone (DEX), a hydrophobic model drug, released comparatively slower than 5-fluoruracil (5-FU), a hydrophilic model drug, due to the potential partitioning of DEX into the PVL core. 5-FU in vitro release from copolymer 2 was 86% in 22 h, whereas only 14% of DEX was released in 24 h. Cell viability studies confirmed that hydrogels composed of block copolymers are biocompatible. Copolymer 2 showed more than 80% relative cell viability at all concentrations, including concentrations greater than 200 fold CMC. In vivo gel formation studies indicate that gel integrity was maintained for 7 days upon subcutaneous injection into mice. These results indicate that PEG–PVL–PEG copolymers are suitable for drug delivery applications. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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19. Microemulsion based Hydrogel of Mycophenolate Mofetil for the Treatment of Psoriasis.
- Author
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Sharma, Kusha and Bedi, Neena
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MYCOPHENOLIC acid , *MICROEMULSIONS , *PSORIASIS treatment , *COLLOIDS in medicine , *DRUG delivery systems , *ANTI-inflammatory agents - Abstract
Mycophenolate mofetil (MMF), the morpholinoethyl ester of mycophenolic acid is an immunosuppressive agent used to prevent organ rejection after kidney and heart transplant. The drug seem to be effective in dermal diseases mainly psoriasis. However, till date it can be only be administered using systemic route which is often associated with side effects such as nausea, leucopenia, sepsis and diarrheoa. The aim of the present study was to develop microemulsion based hydrogel for topical delivery of mycophenolate mofetil and to investigate its in vitro release and its potential in treating psoriatic inflammation using imiquimod induced skin inflammation animal model. Pseudoternary phase diagrams were constructed and on the basis of microemulsion existence range, various formulations were developed using oleic acid, Tween80, propylene glycol and distilled water as oil phase, surfactant, cosurfactant and aqueous phase, respectively. The selected formulations were subjected to physical stability studies and consequently to various physicochemical characterization. The optimized formulation (F2) consisting of 6.06% v/v of oleic acid 36.36% v/v of Tween80 and 18.18% v/v of propylene glycol has shown a globule size of 124 nm, refractive index 1.421, zeta potential -34.35 ±0.051 mV, pH 5.9 and conductivity value of 104 μS cm-1. The permeability of drug from microemulsion after 24 h was observed to be 69.52%. Carbopol 940 was used to convert microemulsion into microemulsion based hydrogel to improve its viscosity for topical administration and was characterized. The histopathological studies performed on mice skin revealed that the treated skin showed complete clearance of hyperkeratotic plaques and a significant reduction in the area of perakeratosis. The results indicated that the formulated gel may be a promising vehicle for topical delivery of mycophenolate mofetil for the treatment of psoriasis. [ABSTRACT FROM AUTHOR]
- Published
- 2014
20. Cytarabine-AOT catanionic vesicle-loaded biodegradable thermosensitive hydrogel as an efficient cytarabine delivery system.
- Author
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Liu, Jing, Jiang, Yue, Cui, Yuting, Xu, Chuanshan, Ji, Xiaoqing, and Luan, Yuxia
- Subjects
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CYTARABINE , *BIODEGRADATION , *COLLOIDS in medicine , *DRUG delivery systems , *DRUG carriers , *ANTINEOPLASTIC agents , *ANIONIC surfactants - Abstract
Carrier with high drug loading content is one of the most important issues in drug delivery system. In the present work, an ion-pair amphiphilic molecule composed of anticancer drug cation and surfactant anion is used for straightforward fabricating vesicles for cancer therapy. Anticancer drug (cytarabine hydrochloride) and anionic surfactant (AOT) are selected for the fabrication of ion-pair amphiphilic molecule. One amphiphilic molecule contains one drug cation, thus the drug loading content is 50% (mol/mol) in theory. The in vitro drug release study shows that the release time of cytarabine is about 3 times of the pure cytarabine solution and the permeability of cytarabine has been improved about 160 times tested by parallel artificial membrane permeability assay model. However, the hemolytic toxicity is largely decreased in the studied concentration range. The in vitro cytotoxicity results show that cytarabine-AOT amphiphiles have a much lower IC 50 (drug concentration resulting in 50% cell death) value and a higher cell inhibition rate comparing with their respective components, indicating its effective therapy for leukemic cells. To obtain a longer and a convenient drug release system, the prepared vesicles are further incorporated into the thermosensitive PLGA–PEG–PLGA hydrogel to prepare a subcutaneous administration. The in vivo drug release results indicate that cytarabine-AOT vesicle-loaded hydrogel is a good injectable delivery system forcontrolled release of cytarabine for cancer therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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21. Stimuli sensitive polymers and self regulated drug delivery systems: A very partial review.
- Author
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Siegel, Ronald A.
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MEDICAL polymers , *DRUG delivery systems , *CONTROLLED release drugs , *STIMULUS & response (Biology) , *COLLOIDS in medicine , *BIOSENSORS , *CLOSED loop systems - Abstract
Since the early days of the Journal of Controlled Release , there has been considerable interest in materials that can release drug on an “on-demand” basis. So called “stimuli-responsive” and “intelligent” systems have been designed to deliver drug at various times or at various sites in the body, according to a stimulus that is either endogenous or externally applied. In the past three decades, research along these lines has taken numerous directions, and each new generation of investigators has discovered new physicochemical principles and chemical schemes by which the release properties of materials can be altered. No single review could possibly do justice to all of these approaches. In this article, some general observations are made, and a partial history of the field is presented. Both open loop and closed loop systems are discussed. Special emphasis is placed on stimuli-responsive hydrogels, and on systems that can respond repeatedly. It is argued that the most success at present and in the foreseeable future is with systems in which biosensing and actuation (i.e. drug delivery) are separated, with a human and/or cybernetic operator linking the two. [ABSTRACT FROM AUTHOR]
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- 2014
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22. Statistically designed nonionic surfactant vesicles for dermal delivery of itraconazole: Characterization and invivo evaluation using a standardized Tinea pedis infection model.
- Author
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Kumar, Neeraj and Goindi, Shishu
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NONIONIC surfactants , *DRUG delivery systems , *ATHLETE'S foot treatment , *CYCLODEXTRINS , *COLLOIDS in medicine , *CHOLESTEROL , *THERAPEUTICS - Abstract
The study aims to statistically develop a hydrogel of itraconazole loaded nonionic surfactant vesicles (NSVs) for circumventing the shortcomings and adverse effects of currently used therapies. Influential factors were screened using first-order Taguchi design, thereafter, optimization was performed via D-optimal design involving screened factors (surfactant type, content and molar ratio of cholesterol: surfactant). Response variables investigated were percent drug entrapment, vesicle size, drug skin retention and permeation in 6 h. Suspensions of NSVs were gelled to improve topical applicability. Characterization of formulations was performed using vesicle shape, size, surface charge, texture analysis and rheology behavior. Ex vivo studies in rat skin depicted that optimized formulation augmented drug skin retention and permeation in 6 h than conventional cream and oily solution of itraconazole. Standardized Tinea pedis model in Wistar rats exhibited in vivo antifungal efficacy of optimized formulation, observed in terms of physical manifestations, fungal-burden score and histopathological profiles. Also, a unique investigation involving studying local oxidative stress of infected paw skins as an indicator of fungal infection was performed. Rapid alleviation of infection in animals treated with optimized hydrogel was observed in comparison to commonly prescribed therapies. Therefore, the optimized NSVs may be a promising and efficient alternative to available antifungal therapies. [ABSTRACT FROM AUTHOR]
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- 2014
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23. Colloidal particles containing labeling agents and cyclodextrins for theranostic applications.
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Zafar, Nadiah, Fessi, Hatem, and Elaissari, Abdelhamid
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COLLOIDS in medicine , *DRUG labeling , *CYCLODEXTRINS in pharmaceutical technology , *COMPANION diagnostics , *DRUG delivery systems , *MACROCYCLIC compounds , *BIOCOMPATIBILITY - Abstract
This review aims to give to the reader some new light on cyclodextrin (CD)-based theranostic agents in order to complete our recently published review dedicated to CD-particles conjugates in drug delivery systems (Zafar et al., 2014). CDs are biocompatible sugar-based macrocycles used in a wide range of biomedical applications. Here, we mainly focus on fundamental theranostic approaches combining the use of cyclodextrin molecules and colloidal particles as theranostic agents. The system's key features are discussed and a few recent pertinent applications are presented. CDs are used in order to enhance theranostic properties by providing apolar cavities for the encapsulation of hydrophobic moieties. Thus, CD molecules are used to enhance the loading capacity of particles by hosting active molecules. The relevance of CDs in enhancing the labeling properties of particles and the preparation of controlled drug release particles is also highlighted. [ABSTRACT FROM AUTHOR]
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- 2014
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24. Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells.
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Maia, F. Raquel, Barbosa, Mariana, Gomes, David B., Vale, Nuno, Gomes, Paula, Granja, Pedro L., and Barrias, Cristina C.
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COLLOIDS in medicine , *DRUG delivery systems , *OSTEOINDUCTION , *MESENCHYMAL stem cells , *PEPTIDE analysis , *DRUG carriers , *HEALTH outcome assessment , *GROWTH factors - Abstract
The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration. [ABSTRACT FROM AUTHOR]
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- 2014
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25. Controlled drug delivery attributes of co-polymer micelles and xanthan-O-carboxymethyl hydrogel particles.
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Maiti, Sabyasachi and Mukherjee, Susweta
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DRUG delivery systems , *CONTROLLED release drugs , *COPOLYMERS , *MICELLES , *XANTHAN gum , *CARBOXYMETHYL compounds , *COLLOIDS in medicine - Abstract
Herein, C 16 alkyl chain- grafted -xanthan copolymer was synthesized and characterized. The copolymer self-assembled into nanometer-size spherical micellar structures in water and incorporated ∼100% glibenclamide into its deeper lipophilic confines. The micellar dispersion exhibited negative zeta potential value (−27.6 mV). The copolymer micelles controlled the drug release rate in phosphate buffer solution (pH 6.8) for an extended period. Further incorporation of drug-loaded copolymer micelles into O -carboxymethyl xanthan hydrogel particles slowed the drug release rate in HCl solution (pH 1.2) as well as in phosphate-buffered solution (pH 6.8) (releasing only ∼8% drug in 2 h). The drug release data correlated well with the degree of swelling of the hydrogel particles in different drug release media. Scanning electron microscopy revealed spherical shape of the hydrogel particles (600 μm). X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy analyses suggested amorphous encapsulation of the drug and its chemical compatibility with the polymers, respectively. Pharmacodynamic evaluation suggested that the formulations had an immense potential in controlling blood glucose level in animal model over a longer duration. In summary, it was pointed out that the copolymer micelles of glibenclamide, a poor water-soluble anti-diabetic, and their subsequent entrapment into hydrogel particles could be a promising approach in the controlled and effective management of diabetes. [ABSTRACT FROM AUTHOR]
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- 2014
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26. Synthesis, characterization, biodegradability and biocompatibility of a temperature-sensitive PBLA-PEG-PBLA hydrogel as protein delivery system with low critical gelation concentration.
- Author
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Xu, Yourui, Shen, Yan, Xiong, Yerong, Li, Chang, Sun, Chunmeng, Ouahab, Ammar, and Tu, Jiasheng
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MEDICAL polymers ,COLLOIDS in medicine ,BLOCK copolymers ,CHEMICAL synthesis ,BIODEGRADABLE plastics ,PROTEIN drugs ,BIOCOMPATIBILITY ,DRUG delivery systems - Abstract
Temperature-sensitive hydrogels were designed using a series of A-B-A triblock copolymers consisting of poly (ethylene glycol) (PEG) with different molecular weights as the hydrophilic block B and poly (β-butyrolactone-co-lactic acid)(PBLA) with varying block lengths and composition as the hydrophobic block A. The triblock copolymers were synthesized by ring-opening polymerization (ROP) of β-BL and LA in bulk using PEG as an initiator and Sn(Oct)
2 as the catalyst. Their chemical structure and molecular characteristics were determined by NMR, GPC and DSC, and the relationship between structure and phase behaviors in aqueous solutions was investigated as well. It was found that the phase behaviors in aqueous solutions including critical micelle concentration (CMC), sol-gel-sedimentation phase transition temperature, gel window width and critical gelation concentration (CGC) are largely dependent on the molecular weight and block length ratio of PEG/PBLA. Most importantly, they show a very low CGC ranging from 4 to 8 wt% because of the introduction of β-BL. Furthermore, the biodegradability and biocompatibility of the hydrogels were evaluated. Finally, lysozyme as a model protein was used to evaluate the ability to deliver protein drugs in a sustained release manner and biologically active form. All results demonstrated that the temperature-sensitive in situ forming hydrogel has a promising potential as sustained delivery system for protein drugs. [ABSTRACT FROM AUTHOR]- Published
- 2014
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27. Chasing bacteria within the cells using levofloxacin-loaded hyaluronic acid nanohydrogels.
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Montanari, E., D'Arrigo, G., Di Meo, C., Virga, A., Coviello, T., Passariello, C., and Matricardi, P.
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FLUOROQUINOLONES , *THERAPEUTIC use of hyaluronic acid , *COLLOIDS in medicine , *NANOMEDICINE , *MEDICAL innovations , *DRUG delivery systems , *THERAPEUTICS - Abstract
In the present work, an innovative approach based on the delivery of levofloxacin (LVF) from polysaccharide nanohydrogels for the treatment of bacterial intracellular infections is described. The nanohydrogels (NHs) were obtained by self-assembling of the hyaluronic acid-cholesterol amphiphilic chains in aqueous environment. LVF, a fluoroquinolone antibiotic scarcely efficient in intracellular infections, was entrapped within such NHs by nanoprecipitation, thus forming a drug delivery system (LVF-NHs) that was tested for its activity on different bacteria strains. The MIC values of levofloxacin-loaded nanohydrogels were determined for Staphylococcus aureus and Pseudomonas aeruginosa strains and compared to those obtained using free LVF. The intracellular antimicrobial activity of LVF-NHs and free LVF was compared on HeLa epithelial cell line infected by the above mentioned bacteria, and the increase in antibacterial efficacy of LVF-NHs with respect to that of free LVF was evidenced. The obtained results allow to conclude that this new approach can be considered as really promising method for intracellular infection treatments. [ABSTRACT FROM AUTHOR]
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- 2014
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28. In Vitro and In Vivo Evaluation of a Hydrogel Reservoir as a Continuous Drug Delivery System for Inner Ear Treatment.
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Hütten, Mareike, Dhanasingh, Anandhan, Hessler, Roland, Stöver, Timo, Esser, Karl-Heinz, Möller, Martin, Lenarz, Thomas, Jolly, Claude, Groll, Jürgen, and Scheper, Verena
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COLLOIDS in medicine , *DRUG delivery systems , *FIBROSIS , *INNER ear diseases , *DEAFNESS , *GLUCOCORTICOIDS , *THERAPEUTICS - Abstract
Fibrous tissue growth and loss of residual hearing after cochlear implantation can be reduced by application of the glucocorticoid dexamethasone-21-phosphate-disodium-salt (DEX). To date, sustained delivery of this agent to the cochlea using a number of pharmaceutical technologies has not been entirely successful. In this study we examine a novel way of continuous local drug application into the inner ear using a refillable hydrogel functionalized silicone reservoir. A PEG-based hydrogel made of reactive NCO-sP(EO-stat-PO) prepolymers was evaluated as a drug conveying and delivery system in vitro and in vivo. Encapsulating the free form hydrogel into a silicone tube with a small opening for the drug diffusion resulted in delayed drug release but unaffected diffusion of DEX through the gel compared to the free form hydrogel. Additionally, controlled DEX release over several weeks could be demonstrated using the hydrogel filled reservoir. Using a guinea-pig cochlear trauma model the reservoir delivery of DEX significantly protected residual hearing and reduced fibrosis. As well as being used as a device in its own right or in combination with cochlear implants, the hydrogel-filled reservoir represents a new drug delivery system that feasibly could be replenished with therapeutic agents to provide sustained treatment of the inner ear. [ABSTRACT FROM AUTHOR]
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- 2014
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29. Mechanical properties of alginate hydrogels manufactured using external gelation.
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Kaklamani, Georgia, Cheneler, David, Grover, Liam M., Adams, Michael J., and Bowen, James
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ALGINATES ,COLLOIDS in medicine ,GELATION ,TISSUE engineering ,DRUG delivery systems ,POLYSACCHARIDES - Abstract
Abstract: Alginate hydrogels are commonly used in biomedical applications such as scaffolds for tissue engineering, drug delivery, and as a medium for cell immobilisation. Multivalent cations are often employed to create physical crosslinks between carboxyl and hydroxyl moieties on neighbouring polysaccharide chains, creating hydrogels with a range of mechanical properties. This work describes the manufacture and characterisation of sodium alginate hydrogels using the divalent cations Mg
2+ , Ca2+ and Sr2+ to promote gelation via non-covalent crosslinks. Gelation time and Young׳s modulus are characterised as a function of cation and alginate concentrations. The implications of this work towards the use of environmental elasticity to control stem cell differentiation are discussed. [Copyright &y& Elsevier]- Published
- 2014
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30. Remote-Controlled Hydrogel Depots for Time-Scheduled Vaccination.
- Author
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Gübeli, Raphael J., Hövermann, Désirée, Seitz, Hanna, Rebmann, Balder, Schoenmakers, Ronald G., Ehrbar, Martin, Charpin‐El Hamri, Ghislaine, Daoud‐El Baba, Marie, Werner, Martin, Müller, Martin, and Weber, Wilfried
- Subjects
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COLLOIDS in medicine , *DRUG administration , *DRUG delivery systems , *PHARMACOLOGY , *LABORATORY mice , *HUMAN papillomavirus vaccines - Abstract
Remote-controlled drug depots represent a highly valuable tool for the timely controlled administration of pharmaceuticals in a patient compliant manner. Here, the first pharmacologically controlled material that allows for the scheduled induction of a medical response in mice is described. To this aim, a novel, humanized biohybrid material that releases its cargo in response to a small-molecule stimulus licensed for human use is developed. The functionality of the material in mice is demonstrated by the remote-controlled delivery of a vaccine against the oncogenic human papillomavirus type 16. It is shown that the biohybrid depot-mediated immunoprotection is equivalent to the classical multi-injection-based vaccination. These results indicate that this material can be used as a universal remote-controlled vehicle for the patient-compliant delivery of vaccines and pharmaceuticals. [ABSTRACT FROM AUTHOR]
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- 2014
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31. Therapeutic Effect of Nanogel-Based Delivery of Soluble FGFR2 with S252W Mutation on Craniosynostosis.
- Author
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Yokota, Masako, Kobayashi, Yukiho, Morita, Jumpei, Suzuki, Hiroyuki, Hashimoto, Yoshihide, Sasaki, Yoshihiro, Akiyoshi, Kazunari, and Moriyama, Keiji
- Subjects
- *
NANOGELS , *COLLOIDS in medicine , *DRUG delivery systems , *FIBROBLAST growth factor receptors , *GENETIC mutation , *CRANIOSYNOSTOSES - Abstract
Apert syndrome is an autosomal dominantly inherited disorder caused by missense mutations in fibroblast growth factor receptor 2 (FGFR2). Surgical procedures are frequently required to reduce morphological and functional defects in patients with Apert syndrome; therefore, the development of noninvasive procedures to treat Apert syndrome is critical. Here we aimed to clarify the etiological mechanisms of craniosynostosis in mouse models of Apert syndrome and verify the effects of purified soluble FGFR2 harboring the S252W mutation (sFGFR2IIIcS252W) on calvarial sutures in Apert syndrome mice in vitro. We observed increased expression of Fgf10, Esrp1, and Fgfr2IIIb, which are indispensable for epidermal development, in coronal sutures in Apert syndrome mice. Purified sFGFR2IIIcS252W exhibited binding affinity for fibroblast growth factor (Fgf) 2 but also formed heterodimers with FGFR2IIIc, FGFR2IIIcS252W, and FGFR2IIIbS252W. Administration of sFGFR2IIIcS252W also inhibited Fgf2-dependent proliferation, phosphorylation of intracellular signaling molecules, and mineralization of FGFR2S252W-overexpressing MC3T3-E1 osteoblasts. sFGFR2IIIcS252W complexed with nanogels maintained the patency of coronal sutures, whereas synostosis was observed where the nanogel without sFGFR2S252W was applied. Thus, based on our current data, we suggest that increased Fgf10 and Fgfr2IIIb expression may induce the onset of craniosynostosis in patients with Apert syndrome and that the appropriate delivery of purified sFGFR2IIIcS252W could be effective for treating this disorder. [ABSTRACT FROM AUTHOR]
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- 2014
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32. Click hydrogels, microgels and nanogels: Emerging platforms for drug delivery and tissue engineering.
- Author
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Jiang, Yanjiao, Chen, Jing, Deng, Chao, Suuronen, Erik J., and Zhong, Zhiyuan
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COLLOIDS in medicine , *NANOGELS , *MICROGELS , *DRUG delivery systems , *TISSUE engineering , *TARGETED drug delivery - Abstract
Abstract: Hydrogels, microgels and nanogels have emerged as versatile and viable platforms for sustained protein release, targeted drug delivery, and tissue engineering due to excellent biocompatibility, a microporous structure with tunable porosity and pore size, and dimensions spanning from human organs, cells to viruses. In the past decade, remarkable advances in hydrogels, microgels and nanogels have been achieved with click chemistry. It is a most promising strategy to prepare gels with varying dimensions owing to its high reactivity, superb selectivity, and mild reaction conditions. In particular, the recent development of copper-free click chemistry such as strain-promoted azide-alkyne cycloaddition, radical mediated thiol-ene chemistry, Diels–Alder reaction, tetrazole-alkene photo-click chemistry, and oxime reaction renders it possible to form hydrogels, microgels and nanogels without the use of potentially toxic catalysts or immunogenic enzymes that are commonly required. Notably, unlike other chemical approaches, click chemistry owing to its unique bioorthogonal feature does not interfere with encapsulated bioactives such as living cells, proteins and drugs and furthermore allows versatile preparation of micropatterned biomimetic hydrogels, functional microgels and nanogels. In this review, recent exciting developments in click hydrogels, microgels and nanogels, as well as their biomedical applications such as controlled protein and drug release, tissue engineering, and regenerative medicine are presented and discussed. [Copyright &y& Elsevier]
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- 2014
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33. Cleavable carbamate linkers for controlled protein delivery from hydrogels.
- Author
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Hammer, Nadine, Brandl, Ferdinand P., Kirchhof, Susanne, and Goepferich, Achim M.
- Subjects
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CARBAMATES , *COLLOIDS in medicine , *PROTEIN transport , *DRUG delivery systems , *MEDICAL polymers , *SODIUM dodecyl sulfate , *LYSOZYMES - Abstract
The reversible attachment of proteins to polymers is one potential strategy to control protein release from hydrogels. In this study, we report the reversible attachment of lysozyme to poly(ethylene glycol) (PEG) by degradable carbamate linkers. Phenyl groups with different substituents were used to control the rate of carbamate hydrolysis and the resulting protein release. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed modification with 1–3 PEG chains per lysozyme molecule. Protein PEGylation and PEG chain elimination occurred without changes in secondary protein structure, as demonstrated by circular dichroism spectroscopy. The lytic activity of lysozyme was restored to 73.4±1.7%–92.5±1.2% during PEG chain elimination. Attached PEG chains were eliminated within 24h to 28days, depending on the used linker molecule. When formulated into hydrogels, a maximum of about 60% of the initial dose was released within 7days to 21days. Linker elimination occurs ‘traceless’, so that the protein is released in its native, unmodified form. Altogether, we believe that tethering proteins by degradable carbamate linkers is a promising strategy to control their release from hydrogels. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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34. PEG–PLGA copolymers: Their structure and structure-influenced drug delivery applications.
- Author
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Zhang, Keru, Tang, Xing, Zhang, Juan, Lu, Wei, Lin, Xia, Zhang, Yu, Tian, Bin, Yang, Hua, and He, Haibing
- Subjects
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DRUG delivery systems , *POLYETHYLENE glycol , *LACTIC acid , *GLYCOLIC acid , *COPOLYMERS , *NANOMEDICINE , *MICELLES , *COLLOIDS in medicine - Abstract
Abstract: In the paper, we begin by describing polyethylene glycol–poly lactic acid-co-glycolic acid (PEG–PLGA) which was chosen as a typical model copolymer for the construction of nano-sized drug delivery systems and also the types of PEG–PLGA copolymers that were eluted. Following this we examine the structure-influenced drug delivery applications including nanoparticles, micelles and hydrogels. After that, the preparation methods for nano-sized delivery systems are presented. In addition, the drug loading mode of PEG–PLGA micelles is divided into three aspects. Finally, the drug release profiles of PEG–PLGA micelles, both in terms of their in vitro and in vivo characteristics, are represented. PEG–PLGA copolymers are very suitable for the construction of micelles as carriers for insoluble drugs. This article reviews the structure and the different structure-influenced applications of PEG–PLGA copolymers, concentrating on the application of PEG–PLGA micelles. [Copyright &y& Elsevier]
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- 2014
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- View/download PDF
35. Influence of additives on a thermosensitive hydrogel for buccal delivery of salbutamol: Relation between micellization, gelation, mechanic and release properties.
- Author
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Zeng, Ni, Dumortier, Gilles, Maury, Marc, Mignet, Nathalie, and Boudy, Vincent
- Subjects
- *
COLLOIDS in medicine , *ADDITIVES , *BUCCAL administration , *DRUG delivery systems , *ALBUTEROL , *GELATION , *CONTROLLED release drugs - Abstract
Thermosensitive hydrogels developed for buccal delivery of salbutamol were prepared using poloxamer analogs (Kolliphor® P407/P188), xanthan gum (Satiaxane® UCX930) and NaCl. P188 increased gelation temperature (T sol–gel) by 2.5–5°C, micellization temperature (<1°C) and gelation time by >3s. To obtain a suitable T sol–gel at 28–34°C, P407 and P188 concentrations were set to 18–19% and 1%. NaCl reduced T sol–gel (>2°C) out of the optimal range. Six formulations containing 0.05–0.1% Satiaxane® fulfilled the temperature criteria. Concerning the gel strength, 1% P188 had no significant effect, NaCl increased it at 20°C, and Satiaxane® enhanced it at 20°C and 37°C. The release study using membrane-less (to mimic oral cavity) and membrane (to mimic buccal mucosa side) methods allowed a complete investigation showing that erosion and diffusion both contributed to the drug release but differed according to the formulation. In the membraneless method, simple P407 formulations had weak ability to retain salbutamol (T 80 =35min). P188 accelerated drug release. NaCl accelerated release in the membraneless method by 5–11min but slightly reduced it in the membrane method. The hydrogels containing Satiaxane® exhibited the slowest release. In the membrane method, combination of P407/P188/Satiaxane® provided a sustained diffusion with a burst effect (T 25 =9.6min, T 80 =97.8min), which provides potential clinical interests. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
36. Evaluation of an Injectable Thermosensitive Hydrogel As Drug Delivery Implant for Ocular Glaucoma Surgery.
- Author
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Xi, Lei, Wang, Tao, Zhao, Feng, Zheng, Qiongjuan, Li, Xiaoning, Luo, Jing, Liu, Ji, Quan, Daping, and Ge, Jian
- Subjects
- *
GEL permeation chromatography , *DRUG delivery systems , *COLLOIDS in medicine , *CARBONATES , *LABORATORY rabbits , *BIODEGRADABLE plastics ,GLAUCOMA surgery - Abstract
In this study, a biodegradable thermo-sensitive hydrogel from poly(trimethylene carbonate)15-F127-poly(trimethylene carbonate)15 (PTMC15-F127-PTMC15) was designed and evaluated as an injectable implant during ocular glaucoma filtration surgery in vivo and in vitro. Mitomycin C (MMC) was loaded into this hydrogel for controlled released to prolong the efficacy and to reduce the long-term toxicity. The properties of the hydrogel were confirmed using 1H NMR and gel permeation chromatography (GPC). Compared to the Pluronic F127 hydrogel, the PTMC15-F127-PTMC15 hydrogel showed a good solution-gel transition temperature at 37°C, a lower work concentration of 5% w/v and a longer mass loss time of more than 2 weeks. The in vitro study showed that the drug could be released from PTMC15-F127-PTMC15 (5% w/v) hydrogel for up to 16 days with only 57% of drug released in the first day. Moreover, the cell toxicity, which was tested via LDH and ANNEXIN V/PI, decreased within 72 h in human tenon's fibroblast cells (HTFs). The in vivo behavior in a rabbit glaucoma filtration surgery model indicated that this hydrogel loaded with 0.1 mg/ml MMC led to a better functional bleb with a prolonged mean bleb survival time (25.5±2.9 days). The scar tissue formation, new collagen deposition and myofibroblast generation appeared to be reduced upon histological and immunohistochemistry examinations, with no obvious side effects and inflammatory reactions. The in vitro and in vivo results demonstrated that this novel hydrogel is a safe and effective drug delivery candidate in ocular glaucoma surgery. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
37. Development of biodegradable antibacterial cellulose based hydrogel membranes for wound healing.
- Author
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Laçin, Nelisa Türkoğlu
- Subjects
- *
BIODEGRADABLE products , *ANTIBACTERIAL agents , *CELLULOSE synthase , *COLLOIDS in medicine , *WOUND healing , *TISSUE engineering , *DRUG delivery systems - Abstract
Cellulose-based hydrogels have wide applications in tissue engineering and controlled delivery systems. In this study, chloramphenicol (CAP) loaded 2,3 dialdehyde cellulose (DABC) hydrogel membranes were prepared, characterized and their antibacterial efficacy was evaluated. Bacterial cellulose (BC) secreted by Acetobacter xylinum was modified to become DABC by oxidation via the sodium metaperiodate method. CAP–BC and CAP–DABC interactions were illustrated via ATR–FTIR analysis. Water retention capacity of BC and DABC membranes were determined as 65.6±1.6% and 5.3±0.3%, respectively. CAP release profiles were determined via HPLC analysis. The drug-loading capacities of BC and DABC membranes were 5mg/cm2 and 0.1mg/cm2, respectively. Membranes released 99–99.5% of the contained CAP within 24h and an initial burst release effect was not observed. In vitro antibacterial tests of BC and DABC, both CAP-loaded, demonstrated their ability to inhibit bacterial growth for a prolonged duration. Antimicrobial effect against bacteria was still prevalent after 3 days of incubation period with disc diffusion tests. The MTT test results reveal that fibroblast adhesion and proliferation on CAP-loaded DABC membranes were noticeably higher than CAP-loaded BC membrane. This newly developed drug containing DABC membranes seem to be highly suitable for wound healing due to its unique properties of biodegradability, biocompatibility, and antimicrobial effectiveness. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
38. A cisplatin slow-release hydrogel drug delivery system based on a formulation of the macrocycle cucurbit[7]uril, gelatin and polyvinyl alcohol.
- Author
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Oun, Rabbab, Plumb, Jane A., and Wheate, Nial J.
- Subjects
- *
CISPLATIN , *CONTROLLED release drugs , *COLLOIDS in medicine , *DRUG delivery systems , *MACROCYCLIC compounds , *TUMOR growth , *CUCURBITACEAE , *GELATIN , *POLYVINYL alcohol , *THERAPEUTICS - Abstract
Abstract: The anticancer drug cisplatin was encapsulated within the cucurbit[7]uril macrocycle to form the host-guest complex: cisplatin@CB[7]. This was then incorporated into gelatin and 0–4% w/v polyvinyl alcohol (PVA)-based hydrogels as slow release drug delivery vehicles. The hydrogels demonstrated predicable swelling and disintegration dependent on the PVA concentration. The hydrogel with the highest PVA content was slower to swell and release drug compared with lower concentrations of PVA. The effect of the hydrogel PVA concentration on in vitro cytotoxicity was examined using A2780/CP70 ovarian cancer cells. Over the 24h drug exposure time used, hydrogels containing 4% PVA showed a 20% decrease in viable cells compared to the control, whereas hydrogels containing 0% and 2% PVA induced an 80% and 45% inhibition of cell growth, respectively. There was no measurable difference in the in vitro cytotoxicity of free cisplatin and cisplatin@CB[7] containing hydrogels. Finally, the in vivo effectiveness of a 2%-PVA hydrogel implanted under the skin of nude mice bearing A2780/CP70 xenografts showed that low dose hydrogels containing cisplatin@CB[7] (30μg equivalent of drug) was just as effective as an intraperitoneal high dose administration of free cisplatin (150μg) at inhibiting tumour growth. [Copyright &y& Elsevier]
- Published
- 2014
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39. Controlled nail delivery of a novel lipophilic antifungal agent using various modern drug carrier systems as well as in vitro and ex vivo model systems.
- Author
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Naumann, Sandy, Meyer, Jean-Philippe, Kiesow, Andreas, Mrestani, Yahya, Wohlrab, Johannes, and Neubert, Reinhard H.H.
- Subjects
- *
CONTROLLED release drugs , *DRUG delivery systems , *DRUG lipophilicity , *ANTIFUNGAL agents , *DRUG carriers , *IN vitro studies , *ONYCHOMYCOSIS , *COLLOIDS in medicine - Abstract
Abstract: The penetration behavior into human nails and animal hoof membranes of a novel antifungal agent (EV-086K) for the treatment of onychomycosis was investigated in this study. The new drug provides a high lipophilicity which is adverse for penetration into nails. Therefore, four different formulations were developed, with particular focus on a colloidal carrier system (CCS) due to its penetration enhancing properties. On the one hand, ex vivo penetration experiments on human nails were performed. Afterwards the human nail plates were cut by cryomicrotome in order to quantify the drug concentration in the dorsal, intermediate and ventral nail layer using high-performance liquid chromatography (HPLC) with UV detection. On the other hand, equine and bovine hoof membranes were used to determine the in vitro penetration of the drug into the acceptor compartment of an online diffusion cell coupled with Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy. In combination, both results should exhibit a correlation between the EV-086K penetration behavior in human nail plates and animal hoof membranes. The investigations showed that the developed CCS could increase drug delivery through the human nail most compared to other formulations (nail lacquer, solution and hydrogel). Using animal hooves in the online diffusion cell, we were able to calculate pharmacokinetic data of the penetration process, especially diffusion and permeability coefficients. Finally, a qualitative correlation between the penetration results of human nails and equine hooves was established. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
40. Peptide hydrogels as mucoadhesives for local drug delivery.
- Author
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Tang, Claire, Miller, Aline F., and Saiani, Alberto
- Subjects
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DRUG delivery systems , *COLLOIDS in medicine , *PEPTIDES , *MOLECULAR self-assembly , *DRUG stability , *IN vitro studies , *THERAPEUTICS - Abstract
Abstract: We have investigated the possibility of using self-assembling peptide-based viscous solutions and hydrogels as mucoadhesives for the improved delivery of drugs to local mucosal surfaces. The stability of the samples under flow after deposition on a mucosal surface mimic was studied using a simplified in vitro model. Subsequently lidocaine and flurbiprofen, two commercial drugs, were incorporated into the viscous solutions and hydrogels and their release properties investigated using the same model. Peptide-based hydrogels showed a good resistance to erosion under flow conditions. Addition of the soluble drug (lidocaine at low pH) resulted in a stiffening of the samples but did not affect the overall peptide release. Although for this drug the conditions were not favourable, improved retention of the drug was observed for the stiffest samples tested. In the case of the insoluble drug (flurbiprofen) the samples mechanical properties were not altered when the drug was incorporated, however the sample stability and peptide release were. For mechanically weaker samples the presence of the drug as insoluble small particles resulted in an increase in their susceptibility to physically erode when a flow of medium was applied over its surface. On the other hand mechanically stronger samples showed an improved resistance to erosion, which resulted in enhanced drug retention. [Copyright &y& Elsevier]
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- 2014
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41. Nanoparticle-Mediated Pulmonary Drug Delivery: A Review.
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Paranjpe, Mukta and Müller-Goymann, Christel C.
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NANOMEDICINE , *DRUG delivery systems , *LUNG physiology , *COLLOIDS in medicine , *DRUG carriers , *DRUG administration - Abstract
Colloidal drug delivery systems have been extensively investigated as drug carriers for the application of different drugs via different routes of administration. Systems, such as solid lipid nanoparticles, polymeric nanoparticles and liposomes, have been investigated for a long time for the treatment of various lung diseases. The pulmonary route, owing to a noninvasive method of drug administration, for both local and systemic delivery of an active pharmaceutical ingredient (API) forms an ideal environment for APIs acting on pulmonary diseases and disorders. Additionally, this route offers many advantages, such as a high surface area with rapid absorption due to high vascularization and circumvention of the first pass effect. Aerosolization or inhalation of colloidal systems is currently being extensively studied and has huge potential for targeted drug delivery in the treatment of various diseases. Furthermore, the surfactant-associated proteins present at the interface enhance the effect of these formulations by decreasing the surface tension and allowing the maximum effect. The most challenging part of developing a colloidal system for nebulization is to maintain the critical physicochemical parameters for successful inhalation. The following review focuses on the current status of different colloidal systems available for the treatment of various lung disorders along with their characterization. Additionally, different in vitro, ex vivo and in vivo cell models developed for the testing of these systems with studies involving cell culture analysis are also discussed. [ABSTRACT FROM AUTHOR]
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- 2014
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42. Injectable Graphene Oxide/Graphene Composite Supramolecular Hydrogel for Delivery of Anti-Cancer Drugs.
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Hu, Xiaohong, Li, Dan, Tan, Huaping, Pan, Chengbin, and Chen, Xiuxia
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GRAPHENE oxide , *COMPOSITE materials , *SUPRAMOLECULAR chemistry , *COLLOIDS in medicine , *ANTINEOPLASTIC agents , *DRUG delivery systems , *CONTROLLED release drugs - Abstract
Injectable hydrogels have attracted a lot of attention in drug delivery, however, their capacity to deliver water-insoluble or hydrophobic anti-cancer drugs is limited. Here, we developed injectable graphene oxide/graphene composite supramolecular hydrogels to deliver anti-cancer drugs. Pluronic F-127 was used to stabilize graphene oxide (GO) and reduced graphene oxide (RGO) in solution, which was mixed with α-cyclodextrin (α-CD) solution to form hydrogels. Native hydrogel was used as control. GO or RGO slightly shortened gelation time. The storage and loss moduli of the hydrogels were tracked by dynamic force measurement. The storage modulus of GO or RGO composite hydrogels was larger than that of the native hydrogel. Hydrogels were unstable in solution and eroded gradually. GO or RGO in Pluronic F-127 solution could potentially improve the solubility of the water-insoluble anti-cancer drug camptothecin (CPT), especially with large drug-loaded CPT amount. Drug release behaviors from solutions and hydrogels were characterized. The nanocomponents (GO or RGO) were able to bind more drug molecules either for CPT or for doxorubicin hydrochloride (DXR) in solution. Therefore, GO or RGO composite hydrogel could potentially enable better controlled and gentler drug release (for both CPT and DXR) than native hydrogel. [ABSTRACT FROM PUBLISHER]
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- 2014
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43. Heparin-functionalized polymeric biomaterials in tissue engineering and drug delivery applications.
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Liang, Yingkai and Kiick, Kristi L.
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HEPARIN ,BIOMATERIALS ,TISSUE engineering ,DRUG delivery systems ,PROTEIN-protein interactions ,NANOMEDICINE ,COLLOIDS in medicine ,ANTICOAGULANTS - Abstract
Abstract: Heparin plays an important role in many biological processes via its interaction with various proteins, and hydrogels and nanoparticles comprising heparin exhibit attractive properties, such as anticoagulant activity, growth factor binding, and antiangiogenic and apoptotic effects, making them great candidates for emerging applications. Accordingly, this review summarizes recent efforts in the preparation of heparin-based hydrogels and formation of nanoparticles, as well as the characterization of their properties and applications. The challenges and future perspectives for heparin-based materials are also discussed. Prospects are promising for heparin-containing polymeric biomaterials in diverse applications ranging from cell carriers for promoting cell differentiation to nanoparticle therapeutics for cancer treatment. [Copyright &y& Elsevier]
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- 2014
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44. An interfacially plasticized electro-responsive hydrogel for transdermal electro-activated and modulated (TEAM) drug delivery.
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Indermun, Sunaina, Choonara, Yahya E., Kumar, Pradeep, du Toit, Lisa C., Modi, Girish, Luttge, Regina, and Pillay, Viness
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COLLOIDS in medicine , *DRUG delivery systems , *POLYACRYLIC acid , *RESPONSE surfaces (Statistics) , *INDOMETHACIN , *GRAVIMETRIC analysis , *THERAPEUTICS - Abstract
Abstract: This paper highlights the use of hydrogels in controlled drug delivery, and their application in stimuli responsive, especially electro-responsive, drug release. electro-conductive hydrogels (ECHs) displaying electro-responsive drug release were synthesized from semi-interpenetrating networks (semi-IPNs) containing a poly(ethyleneimine) (PEI) and 1-vinylimidazole (VI) polymer blend as the novel electro-active species. The semi-IPNs are systems comprised of polyacrylic acid (PAA) and poly(vinyl alcohol) (PVA). This paper attempts to investigate the various attributes of the electro-responsive ECHs, through institution of a statistical experimental design. The construction of a Box–Behnken design model was employed for the systematic optimization of the ECH composition. The design model comprised of three variables, viz. poly(ethyleneimine) volume; 1-vinylimidazole volume; and applied voltage, critical to the success of the formulation. Electro-responsive drug release was determined on formulations exposed to varying environments to ascertain the optimal environment for the said desired release. A comparison method of formulation water content and swelling through gravimetric analysis was also conducted. Matrix resilience profiles were obtained as an insight to the ability of the ECH to revert to its original structure following applied stress. Response surface and contour plots were constructed for various response variables, namely electro-responsive drug release, matrix resilience and degree of swelling. The outcomes of the study demonstrated the success of electro-responsive drug release. The findings of the study can be utilized for the development of electro-responsive delivery systems of other drugs for the safer and effective drug delivery. Volumes of poly(ethyleneimine) (>2.6mL) and 1-vinylimidazole (>0.7mL), resulted in ideal therapeutic electro-responsive drug release (0.8mg) for indomethacin. Lower amounts of poly(ethyleneimine) and amounts of 1-vinylimidazole ranging from 0.2 to 0.74mL are consistent with greater than 1.6mg release per stimulation. Swelling of <25–45% was seen. [Copyright &y& Elsevier]
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- 2014
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45. Role of N-vinyl-2-pyrrolidinone on the thermoresponsive behavior of PNIPAm hydrogel and its release kinetics using dye and vitamin-B12 as model drug.
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Maheswari, B., Jagadeesh Babu, P.E., and Agarwal, Mayank
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PYRROLIDINONES , *COLLOIDS in medicine , *DYES & dyeing , *VITAMIN B12 , *COPOLYMERIZATION , *DRUG delivery systems , *DIFFERENTIAL scanning calorimetry , *DRUG carriers - Abstract
Temperature-sensitive hydrogels hold great promise in biological applications as they can respond to changes in physiological temperature to produce a desired effect like controlled drug delivery. In this study, a series of poly(N-isopropylacrylamide-co-N-vinyl-2-pyrrolidinone) thermosensitive hydrogels were synthesized by radical copolymerization of NIPAm with 1-vinyl-2-pyrrolidinone (NVP). By altering the initial NIPAm/NVP mole ratios, copolymers were synthesized to have their own distinctive lower critical solution temperature which was established using differential scanning calorimetry. The swelling behavior of the hydrogel was analyzed gravimetrically and it was observed that reswelling rate increases with increasing NVP mole ratio. Further characterizations of the hydrogels were per-formed using Fourier transform infrared spectroscopy and scanning electron microscopy. Release kinetics with respect to temperature was studied using methy-lene blue dye solution and vitamin B12. Kinetic modeling of the release profile revealed that the release mechanism is a non-Fickian diffusion mechanism. These results suggested that this material has potential application as intelligent drug carri-ers. The quantities of residual monomers in the PIV4 hydrogel were determined by HPLC method, and the results show almost complete conversion. [ABSTRACT FROM AUTHOR]
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- 2014
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46. Novel controlled drug delivery system for multiple drugs based on electrospun nanofibers containing nanomicelles.
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Hu, Jun, Zeng, Fangfa, Wei, Junchao, Chen, Yong, and Chen, Yiwang
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DRUG delivery systems , *POLYPHARMACY , *ELECTROSPINNING , *COMPOSITE materials , *MICELLES , *COLLOIDS in medicine , *CONTROLLED release drugs , *CANCER chemotherapy - Abstract
This research described a novel composite electrospun nanofibers, which were consisted of MPEG-b-PLA micelles, chitosan, and PEO, realizing controlled release of both hydrophobic and hydrophilic drugs. 5-FU and Cefradine used as model drugs were successfully loaded in the nanofibers. The in vitro studies showed there was a low initial burst release of 5-FU from micelles-loaded nanofibers, and the final release proportion was about 91.4% after continually releasing for 109h. In vitro cytotoxicity studies revealed that 5-FU-loaded nanofibers restrained HepG-2 cells efficiently, and the cell viability was 45.9% after three days of cultivation in solutions containing micelles-loaded nanofibers with 21.6 μg 5-FU. All results suggested that micelles-loaded nanofibers with two kinds of drugs can be used as an effective controlled drug delivery vehicle and may have a bright future in cancer chemotherapy or clinical treatments. [ABSTRACT FROM AUTHOR]
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- 2014
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47. PEG-g-chitosan thermosensitive hydrogel fo r implant drug delivery: cytotoxicity, in vivo degradation and drug release.
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Jiang, Guoqiang, Sun, Jiali, and Ding, Fuxin
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CHITOSAN , *COLLOIDS in medicine , *DRUG delivery systems , *CELL-mediated cytotoxicity , *BIODEGRADATION , *CONTROLLED release drugs , *FIBROSARCOMA , *CELL lines - Abstract
Thermosensitive hydrogels based on chitosan are of great interests for injectable implant drug delivery. The poly(ethylene glycol)-grafted-chitosan (PEG-g-CS) hydrogel was reported as a potential thermosensitive system. The objective of the present study is to evaluate the cytotoxicity, in vivo degradation and drug release of PEG-g-CS hydrogel. Cytotoxicity was evaluated using L929 murine fibrosarcoma cell line. Degradation and drug release in vivo were investigated by subcutaneous injection of the hydrogel into Sprague-Dawley rats. PEG-g-CS polymer exhibits no significant cytotoxicity when its concentration is less than 3 mg mL"1. After being implanted, PEG-g-CS hydrogel maintains its integrity for two weeks and collapses, merging into the tissue, in the third week. It causes moderate inflammatory response but no fibrous encapsulation around the hydrogel is found. The hydrogel presents a three-week sustained release of cyclosporine A with no significant burst release in vitro and produces the effective drug concentration in blood for more than five weeks in vivo, performing almost the same bioavailability to chitosan/glycerophosphate hydrogel. Further modifications of PEG-g-CS hydrogel might be necessary to modulate the degradation and to mitigate the fluctuations in blood drug concentration. [ABSTRACT FROM AUTHOR]
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- 2014
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48. Hybrid scaffold composed of hydrogel/3D-framework and its application as a dopamine delivery system.
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Kang, Kyung Shin, Lee, Soo-In, Hong, Jung Min, Lee, Jin Woo, Cho, Hwa Yeon, Son, Jin H., Paek, Sun Ha, and Cho, Dong-Woo
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DRUG delivery systems , *DOPAMINE agents , *COLLOIDS in medicine , *DRUG carriers , *CELLULAR mechanics , *PARKINSON'S disease treatment - Abstract
Abstract: Cell-based drug delivery systems (DDSs) have been increasingly exploited because cells can be utilized as a continuous drug delivering system to produce therapeutic molecules over a more extended period compared to the simple drug carriers. Although hydrogels have many advantages for this application, their mechanical properties are generally not desirable to structurally protect implanted cells. Here, we present a three-dimensional (3D) hybrid scaffold with a combination of a 3D framework and a hydrogel to enhance the mechanical properties without chemically altering the transport properties of the hydrogel. Based on the 3D Ormocomp scaffold (framework) fabricated by projection-based microstereolithography with defined parameters, we developed a 3D hybrid scaffold by injection of the mixture of cells and the alginate gel into the internal space of the framework. This hybrid scaffold showed the improved mechanical strength and the framework in the scaffold played the role of an adhesion site for the encapsulated cells during the culture period. Additionally, we confirmed its protection of exogenous human cells from acute immune rejection in a mouse model. Eventually, we demonstrated the feasibility of applying this hybrid scaffold to the treatment of Parkinson's disease as a cell-based DDS. Dopamine released from the 3D hybrid scaffolds encapsulating dopamine-secreting cells for 8weeks suggested its clinical applicability. Further study on its long-term efficacy is necessary for the clinical applicability of this 3D hybrid scaffold for the treatment of Parkinson's disease. [Copyright &y& Elsevier]
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- 2014
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49. Fabrication of a composite system combining solid lipid nanoparticles and thermosensitive hydrogel for challenging ophthalmic drug delivery.
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Hao, Jifu, Wang, Xiaodan, Bi, Yanping, Teng, Yufang, Wang, Jianzhu, Li, Fei, Li, Qiankui, Zhang, Jimei, Guo, Fengguang, and Liu, Jiyong
- Subjects
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NANOCOMPOSITE materials , *COLLOIDS in medicine , *DRUG delivery systems , *OPHTHALMIC drugs , *MEDICAL polymers , *THERMAL analysis - Abstract
Abstract: The purpose of this study was to explore a composite thermosensitive in situ gelling formulation using the distribution of solid lipid nanoparticles (SLNs) among poloxamer-based hydrogels as a potential carrier for novel ocular drug delivery. SLNs containing the model drug Resina Draconis were prepared using a melt-emulsion ultrasonication method. A central composite design (CCD) was adopted to screen the thermosensitive hydrogel (THG) formulation. After aqueous SLNs were dispersed into the THG matrices, the physicochemical properties of the SLNs were characterized before and after their incorporation into hydrogels. The in vitro corneal penetration experiment, ocular irritant test and transcorneal mechanism across the cornea have been previously described to predict the feasibility for the proposed ophthalmic application. Finally, the optimal THGs consisted of 27.8% (w/v) poloxamer 407 and 3.55% (w/v) poloxamer 188. The particle size of the SLNs remained within the colloidal range. In vitro corneal penetration studies revealed a nearly steady sustained drug release. The hen's egg test-chorioallantoic membrane (HET-CAM) test indicated that all of the tested polymer systems were non-irritant. Coumarin-6 labeled SLNs formulated into THGs displayed a more homogeneous fluorescence with a deeper penetration intensity into the cornea at various times. Taken together, these results suggest that the SLN-based THG system can be used as a potential vehicle for ocular application. [Copyright &y& Elsevier]
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- 2014
- Full Text
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50. Versatile UCST-based thermoresponsive hydrogels for loco-regional sustained drug delivery.
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Boustta, Mahfoud, Colombo, Pierre-Emmanuel, Lenglet, Sébastien, Poujol, Sylvain, and Vert, Michel
- Subjects
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COLLOIDS in medicine , *DRUG delivery systems , *GLYCINAMIDE , *MEDICAL polymers , *SOL-gel processes , *TEMPERATURE effect - Abstract
Abstract: Poly(N-acryloyl glycinamide) is a neutral polymer that can form gel–sol thermoresponsive systems with upper critical solution temperature in aqueous media. The temperature of the reversible gel–sol transition depends on the molar mass and the concentration of macromolecules. These parameters were combined to adjust the transition temperature slightly above body temperature for the sake of respecting living tissues during the sol form injection using a classical syringe. On contact with local tissues, the injected sol turned rapidly to a gel. The simplicity of the process makes it exploitable to administrate and deliver neutral or ionic drug and especially those that are soluble in aqueous media. The versatility was exemplified from formulations with cobalt acetate, small polymers (MW~2000g/mol), tartrazine and methylene blue dyes and albumin. The model compounds were allowed to diffuse in an isotonic pH=7.4 buffered medium at 37°C. All the release profiles were typical of diffusion control with 100% release within 2 to 3weeks and no obvious burst. The in vitro release of methylene blue from a gel formulation was checked prior to injection in the peritoneal cavity of mice where the release of the dye was monitored visually through tissue and organ colorations. A comparable polymer-free dye solution was used as control. Coloration appeared rapidly in tissues and organs and it was still detectable 52h post injection of the gel whereas it was no longer present at 24h in control mice. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
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