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Your search keyword '"hydrogel nanoparticle"' showing total 4 results
Start Over Descriptor "hydrogel nanoparticle" Topic drug delivery
4 results on '"hydrogel nanoparticle"'

1. Stable formulations of peptide-based nanogels

Author

Elisabetta Rosa, Antonella Accardo, Giancarlo Morelli, Carlo Diaferia, Enrico Gallo, Rosa, E., Diaferia, C., Gallo, E., Morelli, G., and Accardo, A.

Subjects
Drug, media_common.quotation_subject, Pharmaceutical Science, Nanogels, Peptide, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Article, Theranostic Nanomedicine, Analytical Chemistry, lcsh:QD241-441, Peptide-based nanogels, lcsh:Organic chemistry, Drug Discovery, Nanogel formulation, Physical and Theoretical Chemistry, peptide aggregates, media_common, chemistry.chemical_classification, Liposome, Drug Carriers, Molecular Structure, Inverse emulsion, Organic Chemistry, hydrogel nanoparticles, 021001 nanoscience & nanotechnology, Anticancer drug, Peptide aggregate, Oil emulsion, 0104 chemical sciences, Hydrogel nanoparticle, Drug Liberation, chemistry, Chemical engineering, Chemistry (miscellaneous), Doxorubicin, Drug delivery, Self-healing hydrogels, Molecular Medicine, Diagnostic imaging, Emulsions, 0210 nano-technology, Peptides, Hydrophobic and Hydrophilic Interactions
Abstract

Recently, nanogels have been identified as innovative formulations for enlarging the application of hydrogels (HGs) in the area of drug delivery or in diagnostic imaging. Nanogels are HGs-based aggregates with sizes in the range of nanometers and formulated in order to obtain injectable preparations. Regardless of the advantages offered by peptides in a hydrogel preparation, until now, only a few examples of peptide-based nanogels (PBNs) have been developed. Here, we describe the preparation of stable PBNs based on Fmoc-Phe-Phe-OH using three different methods, namely water/oil emulsion (W/O), top-down, and nanogelling in water. The effect of the hydrophilic&ndash, lipophilic balance (HLB) in the formulation was also evaluated in terms of size and stability. The resulting nanogels were found to encapsulate the anticancer drug doxorubicin, chosen as the model drug, with a drug loading comparable with those of the liposomes.

Published
2020

2. Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes

Author

Jana, Siddhartha S., Bharali, Dhruba J., Mani, Prashant, Maitra, Amarnath, Gupta, Chhitar M., and Sarkar, Debi P.

Subjects
*HYDROGELS, *NANOPARTICLES, *DEXTRAN, *PROTEINS
Abstract

Hydrogel nanoparticles of cross-linked polyvinylpyrrolidone (PVP-NP) (35–50 nm in diameter) containing fluoresceinated dextran (FITC-Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes11Process for Producing a Targeted Gene (1997) US Patent 5, 683, 866).). Incubation of these loaded F-virosomes with human hepatoblastoma cells (HepG2) in culture resulted in membrane-fusion-mediated delivery of NPs to the cell cytoplasm, as inferred from the ability of cells to internalize FITC-Dx loaded PVP-NP (PVPf-NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVPf-NP into the HepG2 cells was assured by selective accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalized PVPf-NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP-NP-mediated cytosolic release of water soluble drugs both in vitro and in vivo has been established for the first time. [Copyright &y& Elsevier]

Published
2002
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3. Targeted cytosolic delivery of hydrogel nanoparticles into HepG2 cells through engineered Sendai viral envelopes

Author

Chhitar M. Gupta, Prashant Mani, Dhruba J. Bharali, Siddhartha S. Jana, Amarnath Maitra, and Debi P. Sarkar

Subjects
Hepatoblastoma, Biophysics, CHO Cells, Membrane Fusion, Sendai virus, Biochemistry, Mice, chemistry.chemical_compound, Virosome, Cytosol, Drug Delivery Systems, Viral envelope, Structural Biology, Cricetinae, Tumor Cells, Cultured, Genetics, Fluorescence microscope, Controlled release, Animals, Humans, Particle Size, Endocytotic Process, Molecular Biology, Drug Carriers, Dose-Response Relationship, Drug, biology, Targeted cytosolic delivery, technology, industry, and agriculture, Povidone, Dextrans, Hydrogels, 3T3 Cells, Cell Biology, biology.organism_classification, In vitro, Hydrogel nanoparticle, Dextran, Microscopy, Fluorescence, chemistry, Cytoplasm, Delayed-Action Preparations, Drug delivery, Viral Fusion Proteins, Fluorescein-5-isothiocyanate
Abstract

Hydrogel nanoparticles of cross-linked polyvinylpyrrolidone (PVP-NP) (35–50 nm in diameter) containing fluoresceinated dextran (FITC-Dx) were encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes11Process for Producing a Targeted Gene (1997) US Patent 5, 683, 866).). Incubation of these loaded F-virosomes with human hepatoblastoma cells (HepG2) in culture resulted in membrane-fusion-mediated delivery of NPs to the cell cytoplasm, as inferred from the ability of cells to internalize FITC-Dx loaded PVP-NP (PVPf-NP) in the presence of azide (an inhibitor of the endocytotic process). Introduction of PVPf-NP into the HepG2 cells was assured by selective accumulation of FITC fluorescence in the cytosolic compartment. The structural integrity of the internalized PVPf-NP was also confirmed by fluorescence microscopy and ultracentrifugation analysis. The potential usefulness of PVP-NP-mediated cytosolic release of water soluble drugs both in vitro and in vivo has been established for the first time.

Published
2002
Full Text
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4. Development of Targeted Hydrogel Nanoparticles as Delivery Vehicles for Cancer Therapy and Imaging.

Author

Qin, Ming

Subjects
Hydrogel Nanoparticle, Drug Delivery, Photodynamic Therapy, Methylene Blue, Doxorubicin, Multidrug Resistance
Abstract

Nanotechnology can provide powerful delivery carriers for cancer therapy. This dissertation addressed some key challenges for the development of nano-drug carriers: targeted therapy, multidrug resistance (MDR) and codelivery of multiple kinds of drugs. A variety of novel hydrogel nanoplatforms were developed for cancer therapy and imaging. Two kinds of methylene blue-conjugated polyacrylamide (PAA) nanoparticles (NPs) were developed for targeted photodynamic therapy, where methylene blue (MB) was the photosensitizer. The conjugation of MB onto NPs improved the MB loading, prevented any leaching of MB and avoided MB degradation due to the effects of enzymes. After decoration with a tumor-targeting F3 peptide, the MB-PAA NPs showed a selective cell killing ability towards MDA-MB-435 melanoma cells in vitro, under illumination. A highly engineerable hydrogel NP was developed for the optimal codelivery of a chemodrug, doxorubicin (Dox) and a chemosensitizer, verapamil (Vera), aiming at alleviating tumor MDR. The NP was based on the copolymer of acrylamide (AA) and 2-carboxyethyl acrylate (CEA). Dox and Vera were post-loaded into the respective co(CEA-AA) NPs. We delivered both Dox-NPs and Vera-NPs into the Dox-resistant NCI/ADR-RES cells. This codelivery increased the intracellular accumulation of Dox, and significantly enhanced the cell killing ability of Dox with respect to NCI/ADR-RES cells in vitro. For the targeted delivery of Dox, the co(CEA-AA) NP was further conjugated with F3 peptides via a “click reaction”. The F3 peptide targeting moieties dramatically improved the uptake of the NPs by the nucleolin-overexpressing glioma cell line 9L as well as by the drug-resistant cell line NCI/ADR-RES, correlating with nucleolin-mediated endocytosis. In vitro cytotoxicity results show that the Dox-F3-NPs have a stronger cell killing ability, towards both 9L and NCI/ADR-RES cells, than the nontargeted Dox-NPs. Finally, a tumor-targeted, intensely blue colored, tumor contrast agent was introduced, based on targeted, Coomassie Blue (CB)-linked, PAA NPs. With high loading of CB (7 wt %), the NPs can clearly stain the tumor area, with a tolerable NP concentration. Again, the F3-targeted CB-PAA NPs demonstrated a better contrast delineation effect, as well as a longer retention time in the tumor area, in 9L-glioma bearing rats, compared to nontargeted CB-PAA NPs.

Published
2014

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