1. Improving antiproliferative effect of the nevirapine on Hela cells by loading onto chitosan coated magnetic nanoparticles as a fully biocompatible nano drug carrier.
- Author
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Moghadam NH, Salehzadeh S, Rakhtshah J, Tanzadehpanah H, Moghadam AH, Hajibabaei F, Sharifinia S, Asl SS, and Saidijam M
- Subjects
- HeLa Cells, Humans, Neoplasms metabolism, Neoplasms pathology, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacokinetics, Antineoplastic Agents pharmacology, Cell Proliferation drug effects, Chitosan chemistry, Chitosan pharmacokinetics, Chitosan pharmacology, Coated Materials, Biocompatible chemistry, Coated Materials, Biocompatible pharmacokinetics, Coated Materials, Biocompatible pharmacology, Drug Carriers chemistry, Drug Carriers pharmacokinetics, Drug Carriers pharmacology, Magnetite Nanoparticles chemistry, Magnetite Nanoparticles therapeutic use, Neoplasms drug therapy, Nevirapine chemistry, Nevirapine pharmacokinetics, Nevirapine pharmacology
- Abstract
The freshly prepared magnetic iron oxide nanoparticles (MIONPs) were coated with SiO
2 and then modified with a Si-based linker (SiL) having chlorine atom at the end of its chain. The resulting chlorine functionalized MIONPs were bonded to chitosan (CT) in trimethylamine solution. Then nevirapine (Nev) drug was loaded into above CT-SiL-MIONPs system and resulting Nev-loaded magnetic nanoparticles, Nev@CT-SiL-MIONPs, studied using different techniques. Furthermore, the value of Nev loading efficiency and also controlled delivery effect of Nev@CT-SiL-MIONP particles was determined by UV-vis spectrometer. Interestingly, the above nanomaterial showed a superparamagnetic property with a saturation magnetization value of 35.66 emu/g, indicating that it has an excellent potential application in the treatment of cancer using magnetic targeting drug delivery technology. Furthermore, the in-vitro antiproliferative activity of Nev@CT-SiL-MIONPs against cancer cell line (Hela) was compared with nevirapine using MTT colourimetric assay. The Nev-loaded magnetic nanoparticles were shown to be more effective antiproliferative on Hela cancer cell lines than nevirapine itself. Moreover, in vitro ct-DNA binding studies were investigated by UV-Vis and competitive fluorescence spectroscopies. The results showed that DNA aggregated on Nev-loaded nanoparticles via groove binding mode., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
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