1. Withangulatin I, a new cytotoxic withanolide from Physalis angulata
- Author
-
Zong-Tsi Chen, Shwu-Woan Lee, Min-Hsiung Pan, and Chiu-Ming Chen
- Subjects
Circular dichroism ,Spectrometry, Mass, Electrospray Ionization ,Magnetic Resonance Spectroscopy ,Physalis ,Spectrophotometry, Infrared ,Stereochemistry ,Physalis angulata ,chemistry.chemical_compound ,Withangulatin I ,Cell Line, Tumor ,Drug Discovery ,Cytotoxic T cell ,Humans ,Withangulatin A ,Withanolides ,Antibiotics, Antineoplastic ,biology ,Chemistry ,Plant Extracts ,General Chemistry ,General Medicine ,biology.organism_classification ,Molecular biology ,Antineoplastic Agents, Phytogenic ,In vitro ,Withanolide ,Cell culture ,Doxorubicin ,Camptothecin ,Spectrophotometry, Ultraviolet ,Drug Screening Assays, Antitumor ,Oxidation-Reduction - Abstract
A new withanolide, withangulatin I (2), was isolated from the whole plant of Physalis angulata. Its structure was established as (20S,22R)-15alpha-acetoxy-5beta,6beta-epoxy-14alpha-hydroxy-1,4-dioxo-witha-2,16,24-trienolide on the basis of chemical and spectroscopic methods including 2D-NMR and circular dichroism (CD) experiments. Withangulatin A (1) and withangulatin I (2) were tested for their cytotoxic activities against two human cancer cell lines, colorectal carcinoma COLO 205 and gastric carcinoma AGS, in vitro. Compounds 1 and 2 exhibited inhibitory activities against these two human cancer cells with IC(50) values of 16.6 and 1.8 and 53.6 and 65.4 muM, respectively.
- Published
- 2008