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Respiratory syncytial virus (RSV) is the most prevalent cause of acute lower respiratory infection in young children. Currently, the first RSV vaccines are approved by the FDA. Recently, N6-methyladenosine (m 6 A) RNA methylation has been implicated in the regulation of the viral life cycle and replication of many viruses, including RSV. m 6 A methylation of RSV RNA has been demonstrated to promote replication and prevent anti-viral immune responses by the host. Whether m 6 A is also involved in viral entry and whether m 6 A can also affect RSV infection via different mechanisms than methylation of viral RNA is poorly understood. Here, we identify m 6 A reader YTH domain-containing protein 1 (YTHDC1) as a novel negative regulator of RSV infection. We demonstrate that YTHDC1 abrogates RSV infection by reducing the expression of RSV entry receptor CX3C motif chemokine receptor 1 (CX3CR1) on the cell surface of lung epithelial cells. Altogether, these data reveal a novel role for m 6 A methylation and YTHDC1 in the viral entry of RSV. These findings may contribute to the development of novel treatment options to control RSV infection.