ABSTRACT The COVID-19 pandemic has ushered in unprecedented changes in the lives of humans and their companion animals, impacting their daily routines, social interactions, and stress levels. This study investigates the effect of pandemic-related restrictions, including limited time outside and changes in owner habits, on the stress indicators of 34 dogs and 29 cats. Cortisol levels, total leukocyte counts, eosinophil counts, and behavioral changes have been analyzed to assess how these species are differentially affected by the altered environment and human interactions during the pandemic. The study revealed important behavioral changes in cats while the biochemical and hematological parameters were considerably affected in dogs. Our findings shed light on the unique stress experiences of dogs and cats and underscore the importance of considering individualized strategies for managing their well-being during extraordinary circumstances.