1. URINE PROTEOME IN DOGS AFFECTED BY LEISHMANIASIS AND CUSHING’S SYNDROME
- Author
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FERLIZZA, ENEA, FRACASSI, FEDERICO, DONDI, FRANCESCO, CARPENE', EMILIO, ISANI, GLORIA, A. Buono, A. Cuoghi, E. Bellei, R. Barrera, E.Ferlizza, A.Buono, F.Fracassi, F.Dondi, A. Cuoghi, E. Bellei, R.Barrera, F. Borsetti, E. Carpene, and G.Isani
- Subjects
CUSHING ,lesihmania ,proteome ,dog ,urine - Abstract
Background: Urinary proteome is a topic not deeply investigated in dogs. Leishmaniasis (L) and Cushing’s Syndrome (CS) are frequently associated with persistent proteinuria. Evaluation of urine proteome with sensitive methods is considered a non invasive procedure to monitor the therapy and select novel biomarkers. Objectives: Comparison of urinary electrophoretic profiles between healthy (H) and diseased dogs (L, CS) at the time of diagnosis and during the treatment monitoring. Methods: Urine samples were collected from 49 dogs (12 H, 26 L, 11 CS) and Urine Protein and Albumin to Creatinine ratios (UPC, UAC), HRE and SDS-PAGE were performed. Electrophoretic profiles were analyzed by ImageJ©; proteins were identified by ESI-Q-TOF. Results: UPC and UAC (mean±SD) were significantly higher (p
- Published
- 2013