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Submitted by (edna.saturno@ufrpe.br) on 2016-10-19T17:24:07Z No. of bitstreams: 1 Marcilia Maria Alves de Souza.pdf: 1643173 bytes, checksum: de92227ec0b9b02a73e95528ab1282d2 (MD5) Made available in DSpace on 2016-10-19T17:24:07Z (GMT). No. of bitstreams: 1 Marcilia Maria Alves de Souza.pdf: 1643173 bytes, checksum: de92227ec0b9b02a73e95528ab1282d2 (MD5) Previous issue date: 2012-02-01 Glanders is a disease that affects equids, produced by Burkholderia mallei, a coccobacillus, gram-negative, immobile, and not sporulated anaerobic (Yabuuchi et al., 1992), which can be transmitted to humans, and his agent used as a biological weapon (Ulrich, et al, 2006). Reemerging disease in Brazil since 1999, has been the subject of discussion regarding the serological diagnosis. Complement Fixation Test (CFT) is recommended in the examination program for control and eradication of glanders by the Ministry of Agriculture, Livestock and Supply (BRAZIL, 2004) and the World Organization for Animal Health (OIE) for international trade (OIE 2008) The Objective of this study was to evaluate the results of the Complement Fixation Test using two antigens commercially available and two methods of incubation in sera of horses from a property in Pernambuco. O mormo é uma doença que acomete equídeos, produzida pela Burkholderia mallei, um cocobacilo, Gram-negativo, imóvel, anaeróbio e não esporulado (YABUUCHI et al., 1992), que pode ser transmitida para o homem, e seu agente utilizado como arma biológica (ULRICH, et al, 2006). Doença reemergente no Brasil desde 1999 tem sido motivo de discussão, no que concerne ao diagnóstico sorológico. Fixação do Complemento (FC) é o exame preconizado no programa de controle e erradicação do mormo, pelo Ministério da Agricultura, Pecuária e Abastecimento (BRASIL, 2004) e pela Organização Mundial de Saúde Animal (OIE) para trânsito internacional (OIE 2008). Objetiva-se com este trabalho, avaliar os resultados do Teste de Fixação do Complemento frente a dois antígenos comercialmente disponíveis utilizando dois métodos de incubação, em soros de equinos de uma propriedade em Pernambuco.

Submitted by (edna.saturno@ufrpe.br) on 2016-11-08T16:25:01Z No. of bitstreams: 1 Veronica Arns Silva.pdf: 798716 bytes, checksum: 9cb78ed7b4e51b894bc4cd0afcfd8bce (MD5) Made available in DSpace on 2016-11-08T16:25:01Z (GMT). No. of bitstreams: 1 Veronica Arns Silva.pdf: 798716 bytes, checksum: 9cb78ed7b4e51b894bc4cd0afcfd8bce (MD5) Previous issue date: 2011-02-28 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Shrimp farm can be affected both by non-infectious diseases caused by abiotic agents, as for infectious diseases, and among these viral diseases were responsible for the largest losses in shrimp farming. Small and medium shrimp were collected from four commercial farms in the coastal state of Pernambuco, in the periods of summer and rainy, with the aim to investigate the frequency of diseases in these animals, with the aim to investigate the frequency of diseases in these animals. The investigation of viruses Infectious myonecrosis (IMNV), and Infectious Hipodermal and Hematopoietic Necrosis (IHHNV) and White Spot Syndrome (WSSV) was performed by macroscopic analysis, using wet mount, microscopically for histopathology, and analysis molecular (Polymerase Chain Reaction - PCR and nested PCR), identifying the presence or absence of etiological agents. Viruses causing IHHNV IMNV and were detected as lesions suggestive of these infections were more frequent during the dry season. WSS virus was not detected by nested PCR in the specimens, as well as injuries that just allowed the suspected virus circulation during the study period. Lesions suggestive of bacterial diseases, enteritis hemocitical, as well as the presence of protozoa and epicomensais gregarines were observed in the shrimp examined. Infectious diseases affecting the shrimp are complex events and the continuous and accurate records of the farms are essential for epidemiological studies. These, identifying risk factors and involving them in the analysis are essential to maintain and monitor the health of animals and for the good productivity of crops. Camarões cultivados podem ser acometidos tanto por enfermidades não infecciosas, causadas por agentes abióticos, quanto por enfermidades infecciosas, sendo dentre essas as de etiologia viral as responsáveis pelas maiores perdas na carcinicultura. Camarões pequenos e médios foram coletados de quatro fazendas comerciais no litoral do estado de Pernambuco, nos períodos chuvoso e de estio, com o objetivo de investigar a frequência de enfermidades nesses animais. A investigação das viroses Mionecrose Infecciosa Viral (IMNV), Necrose Hematopoiética e Hipodermal Infecciosa (IHHNV) e a Síndrome da Mancha Branca (WSSV) foi realizada pela análise macroscópica, por meio de exame a fresco, microscopicamente, por meio da histopatologia, e por análises moleculares (Reação em Cadeia da Polimerase – PCR e nested PCR), identificando a presença ou não dos agentes etiológicos. HHNV e IMNV foram detectados, assim como lesões sugestivas dessas viroses foram mais frequentes no período de estio. O WSSV não foi detectado por nested PCR nos espécimes. Também não foram verificadas lesões que permitissem a suspeita da circulação deste vírus no período do estudo.Lesões sugestivas de bacterioses, enterite hemocítica, assim como presença de protozoários epicomensais e gregarinas foram constatados nos camarões analisados. Enfermidades infecciosas que acometem camarões cultivados são eventos complexos e registros exatos e contínuos das fazendas são indispensáveis para os estudos epidemiológicos. Esses, identificando fatores de risco e associando-os nas análises são fundamentais para manter e monitorar a saúde dos animais e para a boa produtividade dos cultivos.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:20:42Z No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Made available in DSpace on 2016-10-13T12:20:42Z (GMT). No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Previous issue date: 2009-02-13 Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively. A carcinicultura mundial tem enfrentado nos últimos anos vários impactos causados por enfermidades, que contribuíram para a queda dos índices de desenvolvimento na produção de camarão marinho cultivado. Além das perdas causadas devido às enfermidades, ressalta-se que algumas espécies de víbrio são agentes zoonóticos, ou seja, podem causar doenças também em humanos. Dada a importância das bactérias do gênero Vibrio para a carcinicultura e saúde pública, objetivou-se avaliar camarões marinhos, associar os achados clínicos com a presença de bactérias do gênero Vibrio e identificar as espécies isoladas da água dos viveiros e de camarões marinhos (Litopenaeus vannamei) cultivados no litoral do estado de Pernambuco. As amostras foram coletadas em quatro carciniculturas do litoral e as análises laboratoriais compreenderam pesquisa e identificação de bactérias do gênero Víbrio no hepatopâncreas e na água dos viveiros, e identificação de alterações através do exame a fresco. Para o isolamento das espécies foi utilizado o meio de cultivo Ágar Tiossulfato Citrato Sais de Bile Sacarose (TCBS) e as colônias selecionadas foram posteriormente submetidas a provas bioquímicas. Foram analisados no total 480 camarões, destes 424 (88,33%) apresentaram um ou mais tipos de alteração. Foi identificado um total de 903 alterações, sendo a mais freqüente a da musculatura (329; 36,43%) e a menos freqüente a alteração dos túbulos do hepatopâncreas (121; 13,40%). Com relação ao ciclo de cultivo, foi observado o maior número de alterações (503; 55,7%) no período de estio. Levando-se em consideração a média de peso dos camarões verificou-se 540 (59,8%) alterações quando os mesmos estavam com peso médio de 8 g. Do total de animais analisados 314 (65,42%) apresentavam vibrio e uma ou mais alteração no exame a fresco. Foi obtido um total de 643 isolados de Vibrio, sendo 38 de amostras de água dos viveiros e 605 isolados de camarão. A espécie mais abundante foi V. mediterranei (21,31%), considerada espécie ambiental e não patogênica para o camarão, e as espécies de menor predominância foram V. campbellii, V. rotiferianus, V. shiloni, V. splendidus, V. tapetis e V. wodanis (0,16%). Foi verificada uma dependência entre as alterações encontradas no exame a fresco e a presença de vibrio, concluindo-se que o referido exame, associado à análise microbiológica é uma importante ferramenta que deve ser utilizada no monitoramento da saúde dos animais. Verificou-se também uma ampla variedade de espécies patogênicas e não patogênicas de víbrio nas amostras estudadas, que podem eventualmente estar relacionados a infecções nos camarões e nos humanos, representando um risco para a carcinicultura e para a saúde pública respectivamente.

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T16:51:11Z No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Made available in DSpace on 2016-08-11T16:51:11Z (GMT). No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Previous issue date: 2007-03-22 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Diseases means any adverse alteration in the health or acting in the productivity of the animals. In shrimps, the diseases can be unchained when it occur an unbalance potentially among the environmental conditions of nurseries, the health condition of the cultivated shrimps and the pathogenic agents, associated with the employed handling. This way, it was aimed at to evaluate the sanitary conditions and to determine which the relevant factors in the attack of the vibrioses in shrimp cultivated Litopenaeus vannamei. In the period of December of 2005 to November of 2006, were collected and analyzed shrimp samples, water and nursery soil, as well as technical information on the handling adopted in a commercial shrimp culture located in the north of Pernambuco/ Brazil. The handling parameters were correlated through mathematical models (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-21T16:11:44Z No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Made available in DSpace on 2017-02-21T16:11:44Z (GMT). No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Previous issue date: 2007-02-22 Considering the illnesses are barriers for the development of the carcinicultura, the vibriose has the possibility to occur for having as etiologic agent bacteria autochtonous of marine environment, it was objectified identification of the interferentes factors in the frequency of this disease. Four situated farms in the south coast of Pernambuco had been selected of which samples of water and shrimp during all had been collected monthly the phases of culture in two different climatic cycles (wet and rainy). Esteem the load of Vibrio spp., samples of water, post larvae had been analyzed, hemolymph and hepatopancreas and the countings had been correlated, through the use of mathematical models (P