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Your search keyword '"Takeshita, Leo"' showing total 1 results
Start Over Author "Takeshita, Leo" Topic dna-binding proteins
1 results on '"Takeshita, Leo"'

1. Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine.

Author

Seio, Kohji, Ohno, Yurie, Ohno, Kentaro, Takeshita, Leo, Kanamori, Takashi, Masaki, Yoshiaki, and Sekine, Mitsuo

Subjects
*CARRIER proteins, *RNA polymerases, *ULTRAVIOLET radiation, *DNA-binding proteins, *DOUBLE-strand polymers
Abstract

Mismatch binding protein MutS binding to bulge structure in DNA duplexes was controlled by UV irradiation. 4- O -(2-Nitrobenzyl)thymidine or 4- O -[2-(2-nitrophenyl)propyl]thymidine was incorporated into DNA duplexes a bulged position. The MutS did not bind to the caged DNA duplexes but bound after removing the 2-nitrobenzyl or 2-(2-nitrophenyl)propyl group by photo-irradiation. By using photo-caged DNA duplex, we revealed that binding of MutS to the uncaged DNA downstream of the T7 RNA promoter weakly inhibited transcription by T7 RNA polymerase. [ABSTRACT FROM AUTHOR]

Published
2016
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