1. Scanning the mitochondrial genome for mutations by cycling temperature capillary electrophoresis
- Author
-
Per Olaf Ekstrøm, Karl Erik Giercksky, Christian Arstad, Paulo Refinetti, and David J. Warren
- Subjects
Male ,0301 basic medicine ,Mitochondrial DNA ,DNA Mutational Analysis ,Mutant ,Mitochondrion ,Biology ,medicine.disease_cause ,DNA, Mitochondrial ,03 medical and health sciences ,symbols.namesake ,chemistry.chemical_compound ,Capillary electrophoresis ,Neoplasms ,Genetics ,medicine ,Humans ,Molecular Biology ,Sanger sequencing ,Mutation ,Electrophoresis, Capillary ,DNA, Neoplasm ,Molecular biology ,030104 developmental biology ,chemistry ,Genome, Mitochondrial ,symbols ,Female ,DNA - Abstract
To bypass possible nuclear contamination and to exclusively amplify DNA from the mitochondrion, a set of 23 primers was selected. On the mitochondrial DNA selection fragments, a second set of fragments was used to amplify and identify mutant fractions with a detection limit of 1% . This mutation scanning method analyzed 76% of the mitochondrial genome and was used to examine 94 tumours from different tissues of origin. In all, 87 tumours had one or more mutations, leaving seven samples without observed mutations. Sanger sequencing verified samples carrying mutations with a mutant fraction exceeding 30%. The generated data validate that several regions of the mitochondrial DNA have more mutations than others.
- Published
- 2016