1. Platform for in situ real-time measurement of protein-induced conformational changes of DNA.
- Author
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Spuhier, Philipp S., Kneževuc, Jelena, Yalçin, Ayca, Bao, Qiuye, Pringsheim, Erika, Dröge, Peter, Rant, Ulrich, and ÜnIü, M. Selim
- Subjects
CONFORMATIONAL analysis ,ESCHERICHIA coli ,PROTEIN binding ,DNA microarrays ,LITHOGRAPHY ,BIOSENSORS - Abstract
A platform for in situ and real-time measurement of proteininduced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia co/i integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay. [ABSTRACT FROM AUTHOR]
- Published
- 2010