1. The effect of c-Fos demethylation on sodium fluoride-induced apoptosis in L-02 cells.
- Author
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Niu Q, Liu H, Guan Z, Zeng Q, Guo S, He P, Guo L, Gao P, Xu B, Xu Z, Xia T, and Wang A
- Subjects
- Blotting, Western, Cell Line, Cell Survival drug effects, CpG Islands, DNA (Cytosine-5-)-Methyltransferase 1, DNA (Cytosine-5-)-Methyltransferases genetics, DNA (Cytosine-5-)-Methyltransferases metabolism, DNA Methyltransferase 3A, Dose-Response Relationship, Drug, Gene Expression Regulation, Hepatocytes enzymology, Hepatocytes metabolism, Humans, Promoter Regions, Genetic, Protein Biosynthesis, Proto-Oncogene Proteins c-fos genetics, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction methods, Sequence Analysis, DNA methods, DNA Methyltransferase 3B, Apoptosis, DNA Methylation, Hepatocytes drug effects, Proto-Oncogene Proteins c-fos metabolism, Sodium Fluoride adverse effects
- Abstract
To investigate the effects of sodium fluoride (NaF) on apoptosis, c-Fos mRNA and protein expression levels, and methylation status as well as Dnmt1, Dnmt3a, and Dnmt3b mRNA expression levels in human embryo hepatocyte (L-02) which were exposed to different concentrations of NaF (0, 20, 40, and 80 mg/l) for 24 h in vitro. Results showed that the percentage of apoptosis and c-Fos mRNA and protein expression levels in 40 and 80 mg/l NaF-treated groups were higher than those in the control group (P<0.05). Further, Dnmt1 mRNA expression level was significantly decreased in the 80 mg/l NaF-treated groups compared to the control group (P<0.05); Dnmt3a and Dnmt3b mRNA expression levels were significantly decreased in 40 and 80 mg/l NaF-treated groups compared to the control group (P<0.05). c-Fos methylation levels, according to the bisulfite sequencing results, were decreased in 20, 40, and 80 mg/l NaF-treated groups against the control group. These results suggest that NaF could induce apoptosis and upregulate mRNA and protein expression level of c-Fos as well as decrease mRNA expression levels of Dnmt1, Dnmt3a, and Dnmt3b in L-02 cells. The decrease in c-Fos methylation levels might be involved in the early phase of apoptosis induced by NaF in L-02 cells.
- Published
- 2012
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