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Start Over Author "Shamas T" Topic dna damage
2 results on '"Shamas T"'

1. Octyl methoxycinnamate modulates gene expression and prevents cyclobutane pyrimidine dimer formation but not oxidative DNA damage in UV-exposed human cell lines.

Author

Duale N, Olsen AK, Christensen T, Butt ST, and Brunborg G

Subjects
Breast Neoplasms drug therapy, Breast Neoplasms genetics, Breast Neoplasms radiotherapy, Cell Line, Tumor, Cell Survival drug effects, Cell Survival radiation effects, DNA, Neoplasm drug effects, DNA, Neoplasm radiation effects, Female, Fibroblasts drug effects, Fibroblasts metabolism, Fibroblasts radiation effects, Gene Expression Regulation radiation effects, Humans, Oxidative Stress drug effects, Oxidative Stress radiation effects, Pyrimidine Dimers radiation effects, Cinnamates pharmacology, DNA Damage drug effects, Gene Expression Regulation drug effects, Pyrimidine Dimers metabolism, Sunscreening Agents pharmacology, Ultraviolet Rays
Abstract

Octyl methoxycinnamate (OMC) is one of the most widely used sunscreen ingredients. To analyze biological effects of OMC, an in vitro approach was used implying ultraviolet (UV) exposure of two human cell lines, a primary skin fibroblast (GM00498) and a breast cancer (MCF-7) cell lines. End points include cell viability assessment, assay of cyclobutane pyrimidine dimers (CPDs) and oxidated DNA lesions using alkaline elution and lesion-specific enzymes, and gene expression analysis of a panel of 17 DNA damage-responsive genes. We observed that OMC provided protection against CPDs, and the degree of protection correlated with the OMC-mediated reduction in UV dose. No such protection was found with respect to oxidative DNA lesions. Upon UV exposure in the presence of OMC, the gene expression studies showed significant differential changes in some of the genes studied and the expression of p53 protein was also changed. For some genes, the change in expression seemed to be delayed in time by OMC. The experimental approach applied in this study, using a panel of 17 genes in an in vitro cellular system together with genotoxicity assays, may be useful in the initial screening of active ingredients in sunscreens.

Published
2010
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2. Octyl Methoxycinnamate Modulates Gene Expression and Prevents Cyclobutane Pyrimidine Dimer Formation but not Oxidative DNA Damage in UV-Exposed Human Cell Lines

Author

Shamas T. Butt, Nur Duale, Gunnar Brunborg, Ann-Karin Olsen, and Terje Christensen

Subjects
IN VITRO Methods and Alternatives to Animals, octyl methoxycinnamate, Cell Survival, Ultraviolet Rays, DNA damage, Breast Neoplasms, Pyrimidine dimer, Biology, Toxicology, medicine.disease_cause, chemistry.chemical_compound, cyclobutane pyrimidine dimers, Cell Line, Tumor, Gene expression, sunscreens, medicine, Humans, Viability assay, Regulation of gene expression, Octyl methoxycinnamate, DNA, Neoplasm, Fibroblasts, UV, Oxidative Stress, Gene Expression Regulation, chemistry, Biochemistry, Cinnamates, Pyrimidine Dimers, gene expression, Female, oxidative DNA lesions, Sunscreening Agents, DNA, Genotoxicity, DNA Damage
Abstract

Octyl methoxycinnamate (OMC) is one of the most widely used sunscreen ingredients. To analyze biological effects of OMC, an in vitro approach was used implying ultraviolet (UV) exposure of two human cell lines, a primary skin fibroblast (GM00498) and a breast cancer (MCF-7) cell lines. End points include cell viability assessment, assay of cyclobutane pyrimidine dimers (CPDs) and oxidated DNA lesions using alkaline elution and lesion-specific enzymes, and gene expression analysis of a panel of 17 DNA damage-responsive genes. We observed that OMC provided protection against CPDs, and the degree of protection correlated with the OMC-mediated reduction in UV dose. No such protection was found with respect to oxidative DNA lesions. Upon UV exposure in the presence of OMC, the gene expression studies showed significant differential changes in some of the genes studied and the expression of p53 protein was also changed. For some genes, the change in expression seemed to be delayed in time by OMC. The experimental approach applied in this study, using a panel of 17 genes in an in vitro cellular system together with genotoxicity assays, may be useful in the initial screening of active ingredients in sunscreens.

Published
2010

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