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Your search keyword '"Favetta, M"' showing total 2 results
Start Over Author "Favetta, M" Topic dna damage
2 results on '"Favetta, M"'

1. Double-face activity of resveratrol in voluntary runners: assessment of DNA damage by comet assay.

Author

Tomasello B, Grasso S, Malfa G, Stella S, Favetta M, and Renis M

Subjects
Case-Control Studies, Comet Assay methods, Deoxyguanosine analogs & derivatives, Deoxyguanosine metabolism, Humans, Hydrogen Peroxide, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear metabolism, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress genetics, Resveratrol, Sedentary Behavior, Antioxidants pharmacology, DNA Damage drug effects, Oxidants pharmacology, Oxidative Stress drug effects, Plant Extracts pharmacology, Running physiology, Stilbenes pharmacology
Abstract

Voluntary runners are subjected to a massive increase in reactive oxygen/nitrogen species production, which can promote different oxidative stress-related diseases such as premature aging, neurodegenerative disorders, and cancer. The aims of this work were to evaluate the following in peripheral blood cells of voluntary runners: (i) DNA status; (ii) susceptibility to the in vitro insult induced by hydrogen peroxide (H(2)O(2)) as a breaking agent; (iii) capabilities of 3,5,4'-trihydroxystilbene (RESV) in counteracting DNA damage. Twenty-five male voluntary runners were compared with 20 sedentary men, as age-matched controls, and DNA status was evaluated with different versions of comet assay: alkaline, neutral, and Fpg enzyme-modified version to measure 8-OH-deoxyguanosine (8-oxo-dG) levels. The H(2)O(2) and/or RESV treatments were performed directly on agarose-embedded cells (atypical comet assay). The results evidenced DNA damage and levels of 8-oxo-dG higher in runners than in sedentary control subjects. The runners' DNA was more prone to the in vitro-induced oxidative insult (200 μM H(2)O(2)) than that of the control group. Resveratrol (100 μM), depending on the individual basal DNA status, was able to switch from antioxidant to pro-oxidant. Our results, on the one hand, validated the proposed in vitro experimental protocol in order to measure individual DNA status. On the other hand, our data point out the importance of monitoring the athletes' redox status before subjecting them to dietary supplementation treatment.

Published
2012
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2. Investigations of DNA damage induction and repair resulting from cellular exposure to high dose-rate pulsed proton beams.

Author

Renis, M., Borghesi, M., Favetta, M., Malfa, G., Manti, L., Romano, F., Schettino, G., Tomasello, B., and Cirrone, G. A. P.

Subjects
DNA damage, DNA repair, PROTON beams, RADIOBIOLOGY, IRRADIATION, CANCER radiotherapy, CELL proliferation
Abstract

Studies regarding the radiobiological effects of low dose radiation, microbeam irradiation services have been developed in the world and today laser acceleration of protons and heavy ions may be used in radiation therapy. The application of different facilities is essential for studying bystander effects and relating signalling phenomena in different cells or tissues. In particular the use of ion beams results advantageous in cancer radiotherapy compared to more commonly used X-rays, since the ability of ions in delivering lethal amount of doses into the target tumour avoiding or limiting damage to the contiguous healthy tissues. At the INFN-LNS in Catania, a multidisciplinary radiobiology group is strategically structured aimed to develop radiobiological research, finalised to therapeutic applications, compatible with the use of high dose laser-driven ion beams. The characteristic non-continuous dose rates with several orders of magnitude of laser-driven ion beams makes this facility very interesting in the cellular systems' response to ultra-high dose rates with non-conventional pulse time intervals cellular studies. Our group have projected to examine the effect of high dose laser-driven ion beams on two cellular types: foetal fibroblasts (normal control cells) and DU145 (prostate cancer cells), studying the modulation of some different bio-molecular parameters, in particular cell proliferation and viability, DNA damage, redox cellular status, morphological alterations of both the cytoskeleton components and some cell organelles and the possible presence of apoptotic or necrotic cell death. Our group performed preliminary experiments with high energy (60 MeV), dose rate of 10 Gy/min, doses of 1, 2, 3 Gy and LET 1 keV/μm on human foetal fibroblasts (control cells). We observed that cell viability was not influenced by the characteristics of the beam, the irradiation conditions or the analysis time. Conversely, DNA damage was present at time 0, immediately following irradiation in a dose-dependent manner. The analysis of repair capability showed that the cells irradiated with 1 and 2 Gy almost completely recovered from the damage, but not, however, 3 Gy treated cells in which DNA damage was not recovered. In addition, the results indicate the importance of the use of an appropriate control in radiobiological in vitro analysis. [ABSTRACT FROM AUTHOR]

Published
2013
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