1. Bricks and blueprints: methods and standards for DNA assembly.
- Author
-
Casini, Arturo, Storch, Marko, Baldwin, Geoffrey S., and Ellis, Tom
- Subjects
- *
DNA analysis , *GENE expression , *CHROMOSOMES , *PLETHORA (Pathology) , *LIGASES - Abstract
DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF