1. Genetic tool development in marine protists: Emerging model organisms for experimental cell biology
- Author
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Deepak Nanjappa, Iñaki Ruiz-Trillo, Christopher J. Howe, Chris Bowler, Mariusz Nowacki, Anastasios D. Tsaousis, Noelia Lander, Christopher L. Dupont, Shinichiro Maruyama, Fulei Luan, Andrew E. Allen, Anna M. G. Novák Vanclová, Pamela A. Silver, Nastasia J. Freyria, Peter von Dassow, Elisabeth Hehenberger, Konomi Fujimura-Kamada, Julius Lukeš, Roberto Docampo, Deborah L. Robertson, Thomas E. Clemente, Sebastián R. Najle, Kathryn J. Coyne, Cristina Aresté, Brittany N. Sprecher, Lu Wang, Eleanna Kazana, Verónica Freire-Benéitez, Vladimír Hampl, Cecilia Balestreri, Isabel C. Nimmo, Mariana Rius, Yoshihisa Hirakawa, Arnab Pain, Jorge Ibañez, Elin Einarsson, Lev Tsypin, Heriberto Cerutti, Adrian C. Barbrook, G. Jason Smith, Alexandra Z. Worden, Jian Guo, Jean Claude Lozano, Virginia P. Edgcomb, Huan Zhang, Andrea Highfield, Isaac Núñez, Fatma Gomaa, Jan Pyrih, Natalia Ewa Janowicz, Sara J. Bender, Ross F. Waller, Tobias von der Haar, François-Yves Bouget, Matus Valach, Amanda Hopes, Luke M. Noble, Paulo A. Garcia, Nicholas A.T. Irwin, Tamara Matute, Jernej Turnšek, Ian Hu, Sandra Pucciarelli, Fernán Federici, Valérie Vergé, R. Ellen R. Nisbet, Miguel Angel Chiurillo, Mark Moosburner, Monika Abedin Sigg, Aaron P. Turkewitz, Albane Ruaud, Angela Piersanti, Sebastian G. Gornik, Peter R. Girguis, Adam C. Jones, Lawrence A. Klobutcher, Claudio H. Slamovits, Elena Casacuberta, Imen Lassadi, Elizabeth C. Cooney, Kodai Fukuda, Nicole King, Manuel Ares, Jonathan Z. Kaye, Lisa Sudek, Patrick Beardslee, Cristina Miceli, Xiaoxue Wen, Estienne C. Swart, Yuu Ishii, Ambar Kachale, Pia A. Elustondo, Esteban R. Haro-Contreras, Patrick J. Keeling, José A. Fernández Robledo, Glen L. Wheeler, Colin Brownlee, Rowena Stern, Joshua S. Rest, Susana A. Breglia, Senjie Lin, Duncan B. Coles, Jackie L. Collier, Drahomíra Faktorová, David S. Booth, April Woods, Binnypreet Kaur, Thomas Mock, Gertraud Burger, Jun Minagawa, Yutaka Hanawa, Zhu-Hong Li, Rachele Cesaroni, Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Faktorová, Drahomíra [0000-0001-9623-2233], Nisbet, R. Ellen R. [0000-0003-4487-196X], Allen, Andrew E. [0000-0001-5911-6081], Ares, Manuel, Jr [0000-0002-2552-9168], Bowler, Chris [0000-0003-3835-6187], Burger, Gertraud [0000-0002-8679-8812], Collier, Jackie L. [0000-0001-8774-5715], Cooney, Elizabeth C. [0000-0001-7435-7609], Docampo, Roberto [0000-0003-4229-8784], Dupont, Christopher L. [0000-0002-0896-6542], Edgcomb, Virginia [0000-0001-6805-381X], Freyria, Nastasia J. [0000-0002-4972-9383], Gornik, Sebastian G. [0000-0002-8026-1336], Howe, Christopher J. [0000-0002-6975-8640], Hu, Ian [0000-0002-2287-031X], Ishii, Yuu [0000-0003-1735-9557], Jones, Adam C. [0000-0001-7521-1863], Lin, Senjie [0000-0001-8831-6111], Maruyama, Shinichiro [0000-0002-1128-5916], Minagawa, Jun [0000-0002-3028-3203], Najle, Sebastián R. [0000-0002-0654-9147], Nowacki, Mariusz [0000-0003-4894-2905], Pain, Arnab [0000-0002-1755-2819], Silver, Pamela A. [0000-0002-7856-4071], Jason Smith, G. [0000-0003-1258-4800], Sprecher, Brittany N. [0000-0001-5032-3834], Tsaousis, Anastasios D. [0000-0002-5424-1905], Tsypin, Lev [0000-0002-0642-8468], Turkewitz, Aaron [0000-0003-3531-5806], Turnšek, Jernej [0000-0002-9056-3565], Valach, Matus [0000-0001-8689-0080], von der Haar, Tobias [0000-0002-6031-9254], Mock, Thomas [0000-0001-9604-0362], Worden, Alexandra Z. [0000-0002-9888-9324], Lukeš, Julius [0000-0002-0578-6618], Apollo - University of Cambridge Repository, Nisbet, R Ellen R [0000-0003-4487-196X], Allen, Andrew E [0000-0001-5911-6081], Ares, Manuel [0000-0002-2552-9168], Collier, Jackie L [0000-0001-8774-5715], Cooney, Elizabeth C [0000-0001-7435-7609], Dupont, Christopher L [0000-0002-0896-6542], Freyria, Nastasia J [0000-0002-4972-9383], Gornik, Sebastian G [0000-0002-8026-1336], Howe, Christopher J [0000-0002-6975-8640], Jones, Adam C [0000-0001-7521-1863], Najle, Sebastián R [0000-0002-0654-9147], Silver, Pamela A [0000-0002-7856-4071], Jason Smith, G [0000-0003-1258-4800], Sprecher, Brittany N [0000-0001-5032-3834], Tsaousis, Anastasios D [0000-0002-5424-1905], Worden, Alexandra Z [0000-0002-9888-9324], Gordon and Betty Moore Foundation, Leverhulme Trust, Ministry of Education, Youth and Sports (Czech Republic), and European Commission
- Subjects
Resource ,Molecular biology ,Range (biology) ,Tree of life (biology) ,[SDV]Life Sciences [q-bio] ,Green Fluorescent Proteins ,ved/biology.organism_classification_rank.species ,Marine Biology ,Environment ,Diversification (marketing strategy) ,Biology ,Models, Biological ,Biochemistry ,Genome ,03 medical and health sciences ,Transformation, Genetic ,0302 clinical medicine ,Species Specificity ,Genome editing ,631/1647/767/722 ,Ecosystem ,14. Life underwater ,Model organism ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0303 health sciences ,Dna delivery ,030306 microbiology ,ved/biology ,Cellular pathways ,Eukaryota ,Genetic systems ,Biodiversity ,DNA ,Cell Biology ,Genetic models ,biology.organism_classification ,Cell biology ,QR ,Transformation (genetics) ,Eukaryote ,030217 neurology & neurosurgery ,631/337 ,Biotechnology - Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways., This collaborative effort was supported by the Gordon and Betty Moore Foundation EMS Program of the Marine Microbiology Initiative (grant nos. GBMF4972 and 4972.01 to F.-Y.B.; GBMF4970 and 4970.01 to M.A. and A.Z.W.; GBMF3788 to A.Z.W.; GBMF 4968 and 4968.01 to H.C.; GBMF4984 to V.H.; GBMF4974 and 4974.01 to C. Brownlee; GBMF4964 to Y. Hirakawa; GBMF4961 to T. Mock; GBMF4958 to P.S.; GBMF4957 to A.T.; GBMF4960 to G.J.S.; GBMF4979 to K.C.; GBMF4982 and 4982.01 to J.L.C.; GBMF4964 to P.J.K.; GBMF4981 to P.v.D.; GBMF5006 to A.E.A.; GBMF4986 to C.M.; GBMF4962 to J.A.F.R.; GBMF4980 and 4980.01 to S.L.; GBMF 4977 and 4977.01 to R.F.W.; GBMF4962.01 to C.H.S.; GBMF4985 to J.M.; GBMF4976 and 4976.01 to C.H.; GBMF4963 and 4963.01 to V.E.; GBMF5007 to C.L.D.; GBMF4983 and 4983.01 to J.L.; GBMF4975 and 4975.01 to A.D.T.; GBMF4973 and 4973.01 to I.R.-T. and GBMF4965 to N.K.), by The Leverhulme Trust (RPG-2017-364) to T. Mock and A. Hopes, and by ERD funds (16_019/0000759) from the Czech Ministry of Education to J.L.
- Published
- 2020