1. Impact of DNA sequence and oligonucleotide length on a polythiophene-based fluorescent DNA biosensor.
- Author
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Charlebois I, Gravel C, Arrad N, Boissinot M, Bergeron MG, and Leclerc M
- Subjects
- Base Sequence, DNA Probes metabolism, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Biosensing Techniques methods, DNA analysis, Oligonucleotides chemistry, Polymers chemistry, Thiophenes chemistry
- Abstract
DNA hybridization is a universal and specific mechanism for the recognition of biological targets. Some cationic polythiophene transducers sensitive to DNA structure have been previously utilized to detect such biomolecules. Further characterization of these systems indicates that both DNA sequence composition and length modulate the biosensor performance. It appears that different repeated sequence patterns cause different conformational changes of the polythiophene, from a more relaxed form to an extremely rigid one. A length difference between the DNA oligonucleotide probe and target has a detrimental effect on the fluorescent signal, but it can be attenuated by changing the sequence composition of the protruding target sequence. This demonstrates that the nature of DNA can be critical for hybridization-based detection systems., (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2013
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