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Your search keyword '"Kalnina, L."' showing total 3 results
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3 results on '"Kalnina, L."'

1. [Activity of "Ferrovir" preparation towards RNA and DNA viruses].

Author

Nosik DN, Nosik NN, Kaplina EN, Kalnina LB, Kiseleva IA, Kondrashina NG, Sato Sh, and L'vov DK

Subjects
Animals, Cells, Cultured, Cytomegalovirus Infections virology, DNA Viruses drug effects, Drug Therapy, Combination, Fibroblasts virology, Fishes, HIV Infections virology, Herpes Simplex virology, Humans, Mice, RNA Viruses drug effects, Zidovudine pharmacology, Antiviral Agents pharmacology, Cytomegalovirus drug effects, Cytomegalovirus Infections drug therapy, DNA pharmacology, Ferric Compounds pharmacology, HIV Infections drug therapy, HIV-1 drug effects, Herpes Simplex drug therapy, Herpesvirus 1, Human drug effects
Abstract

Ferrovir (trivalent iron in complex with native sturgeon milt DNA) is nontoxic, its 50% inhibiting concentration (IC50) is at least 4000 micrograms/ml, 90% effective concentration (EC90) towards HIV-1 is 800 micrograms/ml. These effects do not depend on the cell culture or individual biological characteristics and subtypes of 7 strains of HIV-1 used in our study. The chemotherapeutic index of the drug is more than 20. Combined therapy with ferrovir and retrovir had an additive antiviral effect. Ferrovir reduced the titer of human CMV in fibroblast culture by 1-2 Ig TCD50. Ferrovir protected mice after intracerebral inoculation with lethal herpes simplex virus (type 1) (survival 33.7%, protection 27.1%, which is close to the reference group treated with zovirax). These facts evidence antiviral activity of ferrovir towards RNA and DNA viruses and prompt further study of this drug with the aim of its clinical application.

Published
2002

2. A Fe(3+)/DNA complex induces an anti-human immunodeficiency virus factor(s) in CD4+ lymphocyte cell lines.

Author

Nossik D, Kaplina E, Nossik N, Kalnina L, Tsutsumi R, Miura Y, Sera K, Itoh C, Sato S, and Lvov D

Subjects
Animals, Biological Factors pharmacology, CD4-Positive T-Lymphocytes drug effects, Cell Line, Cytokines metabolism, Giant Cells drug effects, Giant Cells virology, Humans, RNA, Messenger analysis, Reverse Transcriptase Polymerase Chain Reaction, Salmon, Anti-HIV Agents pharmacology, Biological Factors biosynthesis, CD4-Positive T-Lymphocytes metabolism, DNA pharmacology, HIV-1 drug effects, Iron pharmacology
Abstract

Numerous cytokines and chemokines are involved in inflammatory and immune response. Whereas some of them inhibit virus replication in vitro directly or increase the patients' T4-lymphocyte level, others effects are not so clear. Using human immunodeficiency virus (HIV) and cell cultures we have studied the antiviral effect of complexes of salmon DNA with metals and of a new factor(s) (antiviral factor, AVF) induced in cells by the complexes. The Fe3+/DNA complex possessed the highest antiviral activity. It was found that MT-2, MT-4, CEM and Jurkat cells treated with the complexes secreted AVF which inhibited the replication of nine HIV-1 isolates, was noncytotoxic and stimulated cell proliferation. AVF did not inactivate HIV. The molecular mass analysis of AVF showed that its antiviral activity is associated with its fraction of M(r) of 3 K. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA from MT-4 cells treated with the complexes showed an increase in the the expression of genes for interleukin-1 alpha (IL-1 alpha), tumour necrosis factor alpha (TNF-alpha) and TNF-beta while expression of genes for IL-1-beta, IL-2, IL-4, IL-6, IL-8. IL-10, IL-12; 35p, 40p, IL-13, GMCSF, GSF and RANTES was not detected at all. However, the anti-HIV activity of the cell culture supernatant in vitro cannot be explained by mere presence of the inflammatory substances mentioned above, because they do not possess such activity and their M(r) is higher than that of AVF. Our findings raise the possibility that AVF(s) may be involved in the mechanism of cell resistance against HIV.

Published
1999

3. A Fe(3+)/DNA complex induces an anti-human immunodeficiency virus factor(s) in CD4+ lymphocyte cell lines

Author

Nossik D, Kaplina E, Nossik N, Kalnina L, Tsutsumi R, Miura Y, Sera K, Itoh C, Sato S, and D.K. Lvov

Subjects
CD4-Positive T-Lymphocytes, Anti-HIV Agents, Reverse Transcriptase Polymerase Chain Reaction, Iron, DNA, Giant Cells, Cell Line, Biological Factors, Salmon, HIV-1, Animals, Cytokines, Humans, RNA, Messenger
Abstract

Numerous cytokines and chemokines are involved in inflammatory and immune response. Whereas some of them inhibit virus replication in vitro directly or increase the patients' T4-lymphocyte level, others effects are not so clear. Using human immunodeficiency virus (HIV) and cell cultures we have studied the antiviral effect of complexes of salmon DNA with metals and of a new factor(s) (antiviral factor, AVF) induced in cells by the complexes. The Fe3+/DNA complex possessed the highest antiviral activity. It was found that MT-2, MT-4, CEM and Jurkat cells treated with the complexes secreted AVF which inhibited the replication of nine HIV-1 isolates, was noncytotoxic and stimulated cell proliferation. AVF did not inactivate HIV. The molecular mass analysis of AVF showed that its antiviral activity is associated with its fraction of M(r) of 3 K. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA from MT-4 cells treated with the complexes showed an increase in the the expression of genes for interleukin-1 alpha (IL-1 alpha), tumour necrosis factor alpha (TNF-alpha) and TNF-beta while expression of genes for IL-1-beta, IL-2, IL-4, IL-6, IL-8. IL-10, IL-12; 35p, 40p, IL-13, GMCSF, GSF and RANTES was not detected at all. However, the anti-HIV activity of the cell culture supernatant in vitro cannot be explained by mere presence of the inflammatory substances mentioned above, because they do not possess such activity and their M(r) is higher than that of AVF. Our findings raise the possibility that AVF(s) may be involved in the mechanism of cell resistance against HIV.

Published
2000

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