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Invited for the cover of this issue are Takashi Kyotani, Tetsuji Itoh and co-workers at Tohoku University, Gunma University and AIST. The image depicts the synthesis of water-dispersible carbon nano-test tubes by using a template technique and the selective adsorption of DNA into the inner space of these test tubes. Read the full text of the article at 10.1002/chem.202301422., (© 2023 Wiley-VCH GmbH.)

We studied the stabilities of short (4- and 3-bp) DNA duplexes within silica mesopores modified with a positively charged trimethyl aminopropyl (TMAP) monolayer (BJH pore diameter 1.6-7.4 nm). The DNA fragments with fluorescent dye were introduced into the pores, and their fluorescence resonance energy transfer (FRET) response was measured to estimate the structuring energies of the short DNA duplexes under cryogenic conditions (temperature 233-323 K). The results confirmed the enthalpic stability gain of the duplex within size-matched pores (1.6 and 2.3 nm). The hybridization equilibrium constants found for the size-matched pores were 2 orders of magnitude larger than those for large pores (≥3.5 nm), and this size-matching effect for the enhanced duplex stability was explained by a tight electrostatic interaction between the duplex and the surface TMAP groups. These results indicate the requirement of the precise regulation of mesopore size to ensure the stabilization of hydrogen-bonded supramolecular assemblies.

Invited for the cover of this issue are Takashi Kyotani, Tetsuji Itoh and co‐workers at Tohoku University, Gunma University and AIST. The image depicts the synthesis of water‐dispersible carbon nano‐test tubes by using a template technique and the selective adsorption of DNA into the inner space of these test tubes. Read the full text of the article at 10.1002/chem.202301422. [ABSTRACT FROM AUTHOR]

Keywords: Ca2+-mediated electrostatic interaction; carbon nano-test tube; DNA adsorption; DNA enrichment; nano-container EN Ca2+-mediated electrostatic interaction carbon nano-test tube DNA adsorption DNA enrichment nano-container 1 1 1 09/25/23 20230921 NES 230921 B Carbon nano-test tubes (CNTTs) can be prepared by a CVD technique b using the straight and uniform nanochannels of an anodic aluminium oxide film as a template (top left). Front Cover: Water-Dispersible Carbon Nano-Test Tubes as a Container for Concentrated DNA Molecules (Chem. Eur. J. 53/2023) Ca2+-mediated electrostatic interaction, carbon nano-test tube, DNA adsorption, DNA enrichment, nano-container. [Extracted from the article]

We examined 24 microsatellite markers to select those most suitable for individual identification and parentage tests of brown bears ( Ursus arctos) based on statistical parameters and experimental error using liver samples (38 issues) from nuisance bears killed in the Akan–Shiranuka Region, eastern Hokkaido, Japan from 1996 to 2006. We found seven microsatellite markers suitable for identifying individuals (G1A, G10B, G10L, UarMU5, UarMU23, UarMU50, and UarMU51; probability of identity Pid = 3.17 × 10−7; probability of identity of sibs Pid-sib = 2.23 × 10−3), 12 microsatellite markers suitable for parentage testing when one parent is known [G1A, G1D, G10B, G10L, G10P, UarMU5, UarMU23, UarMU50, UarMU51, UarMU59, UarMU61, and UarMU64; probability of excluding one parent (PE I = 0.9991)], and 15 microsatellite markers for parentage testing when neither parents are known [three markers added to the above: G10M, G10X, and UarMU9; probability of excluding both parents (PE II = 0.9869)]. [ABSTRACT FROM AUTHOR]