17 results on '"Guo, Qiuping"'
Search Results
2. Design of a Modular DNA Triangular-Prism Sensor Enabling Ratiometric and Multiplexed Biomolecule Detection on a Single Microbead.
- Author
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Liu Y, Chen Q, Liu J, Yang X, Guo Q, Li L, Liu W, and Wang K
- Subjects
- Microscopy, Atomic Force, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Optical Imaging, Particle Size, Biosensing Techniques, DNA analysis, Nanostructures analysis
- Abstract
DNA nanostructures have emerged as powerful and versatile building blocks for the construction of programmable nanoscale structures and functional sensors for biomarker detection, disease diagnostics, and therapy. Here we integrated multiple sensing modules into a single DNA three-dimensional (3D) nanoarchitecture with a triangular-prism (TP) structure for ratiometric and multiplexed biomolecule detection on a single microbead. In our design, the complementary hybridization of three clip sequences formed TP nanoassemblies in which the six single-strand regions in the top and bottom faces act as binding sites for different sensing modules, including an anchor module, reference sequence module, and capture sequence module. The multifunctional modular TP nanostructures were thus exploited for ratiometric and multiplexed biomolecule detection on microbeads. Microbead imaging demonstrated that, after ratiometric self-calibration analysis, the imaging deviations resulting from uneven fluorescence intensity distribution and differing probe concentrations were greatly reduced. The rigid nanostructure also conferred the TP as a framework for geometric positioning of different capture sequences. The inclusion of multiple targets led to the formation of sandwich hybridization structures that gave a readily detectable optical response at different fluorescence channels and distinct fingerprint-like pattern arrays. This approach allowed us to discriminate multiplexed biomolecule targets in a simple and efficient fashion. In this module-designed strategy, the diversity of the controlled DNA assembly coupled with the geometrically well-defined rigid nanostructures of the TP assembly provides a flexible and reliable biosensing approach that shows great promise for biomedical applications.
- Published
- 2017
- Full Text
- View/download PDF
3. Amplified fluorescence detection of DNA based on catalyzed dynamic assembly and host-guest interaction between β-cyclodextrin polymer and pyrene.
- Author
-
Huang H, Yang X, Wang K, Wang Q, Guo Q, Huang J, Liu J, Guo X, Li W, and He L
- Subjects
- Catalysis, DNA chemistry, Fluorescence, Nucleic Acid Amplification Techniques, Polymers chemistry, DNA analysis, DNA Probes, Pyrenes chemistry, beta-Cyclodextrins chemistry
- Abstract
The detection of nucleic acids is fundamental for studying their functions and for the development of biological studies and medical diagnostics. Herein, we report a new strategy for nucleic acid amplified detection by combining target-catalyzed dynamic assembly with host-guest interaction between β-cyclodextrin polymer (β-CDP) and pyrene. In this strategy, a metastable pyrene-labeled hairpin DNA probe (probe H1) and a metastable unlabeled hairpin DNA probe (probe H2) were elaborately designed as the assembly components, which were kinetically handicapped from cross-opening in the absence of target DNA. In this state, pyrene labled at the 5'-termini of single-stranded stem of probe H1 would be easily trapped into the hydrophobic cavity of β-CDP because of weak steric hindrance, leading to significant fluorescence enhancement. Once the dynamic assembly was catalyzed by target DNA, a hybridized DNA duplex H1-H2 would be created continuously. In this state, it is difficult for pyrene to enter the cavity of β-CDP due to steric hindrance and weak-binding interaction, leading to a weak fluorescent signal. Thus, target DNA could be detected by this simple mix-and-detect amplification method without the need of expensive and perishable protein enzymes. As low as 10 pM of the target DNA was detected by this assay, which was comparable to that of some reported enzyme-dependent amplification methods. Meanwhile, the proposed method was further successfully applied to detect DNA in cell lysate samples, showing great potential for target detection from complex fluids. In addition, as a novel transformation of dynamic DNA assembly technology into enzyme-free signal-amplification analytical application, the proposed strategy has shown great potential for applications in a wide range of fields, such as aptamer-based non-nucleic acid target sensing, biomedicine and bioimaging., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
4. A multiple amplification strategy for nucleic acid detection based on host-guest interaction between the β-cyclodextrin polymer and pyrene.
- Author
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Guo X, Liu P, Yang X, Wang K, Wang Q, Guo Q, Huang J, Liu J, Song C, and Li W
- Subjects
- Humans, Limit of Detection, MicroRNAs blood, beta-Cyclodextrins chemistry, Cellulose chemistry, Cyclodextrins chemistry, DNA analysis, MicroRNAs analysis, Nucleic Acid Amplification Techniques methods, Pyrenes chemistry
- Abstract
A multiple amplification strategy has been developed for nucleic acid detection based on host-guest interaction between the β-cyclodextrin polymer (β-CDP) and pyrene. Briefly, the detection system consists of three parts: the polymerase and nicking enzyme-assisted isothermal strand displacement amplification (SDA) activated by a target DNA or microRNA; the exonuclease III-aided cyclic enzymatic amplification (CEA); and the fluorescence enhancement effect based on host-guest interaction between β-CDP and pyrene. This strategy showed a good positive linear correlation with target DNA concentrations in the range from 75 fM to 1 pM with a detection limit of 41 fM. Significantly, our amplification platform was further validated and evaluated successfully by assaying miRNA-21 in human serum. The proposed assay has great potential as a nucleic acid quantification method for use in biomedical research, clinical analysis and disease diagnostics.
- Published
- 2015
- Full Text
- View/download PDF
5. Concatemeric dsDNA-templated copper nanoparticles strategy with improved sensitivity and stability based on rolling circle replication and its application in microRNA detection.
- Author
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Xu F, Shi H, He X, Wang K, He D, Guo Q, Qing Z, Yan L, Ye X, Li D, and Tang J
- Subjects
- DNA Primers, DNA Replication, DNA, Circular chemistry, DNA, Concatenated chemistry, Fluorescent Dyes, Sensitivity and Specificity, Copper chemistry, DNA chemistry, Metal Nanoparticles chemistry, MicroRNAs analysis
- Abstract
DNA-templated copper nanoparticles (CuNPs) have emerged as promising fluorescent probes for biochemical assays, but the reported monomeric CuNPs remain problematic because of weak fluorescence and poor stability. To solve this problem, a novel concatemeric dsDNA-templated CuNPs (dsDNA-CuNPs) strategy was proposed by introducing the rolling circle replication (RCR) technique into CuNPs synthesis. In this strategy, a short oligonucleotide primer could trigger RCR and be further converted to a long concatemeric dsDNA scaffold through hybridization. After the addition of copper ions and ascorbate, concatemeric dsDNA-CuNPs could effectively form and emit intense fluorescence in the range of 500-650 nm under a 340 nm excitation. In comparison with monomeric dsDNA-CuNPs, the sensitivity of concatemeric dsDNA-CuNPs was greatly improved with ~10,000 folds amplification. And their fluorescence signal was detected to reserve ~60% at 2.5 h after formation, revealing ~2 times enhanced stability. On the basis of these advantages, microRNA let-7d was selected as the model target to testify this strategy as a versatile assay platform. By directly using let-7d as the primer in RCR, the simple, low-cost, and selective microRNA detection was successfully achieved with a good linearity between 10 and 400 pM and a detection limit of 10 pM. The concatemeric dsDNA-CuNPs strategy might be widely adapted to various analytes that can directly or indirectly induce RCR.
- Published
- 2014
- Full Text
- View/download PDF
6. Label-free DNAsensor with PCR-like sensitivity based on background reduction and target-triggered polymerization amplification.
- Author
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Su H, Meng X, Guo Q, Tan Y, Cai Q, Qin H, and Meng X
- Subjects
- Exodeoxyribonucleases chemistry, Fluorescent Dyes, Humans, Polymerase Chain Reaction, Polymerization, Biosensing Techniques methods, DNA chemistry, Hepacivirus isolation & purification, Hepatitis C diagnosis, RNA, Viral isolation & purification
- Abstract
In this work, we have developed a highly sensitive and label-free DNAsensor for nucleic acid detection based on background noise reduction by exonuclease I (Exo I) and target-triggered cycled polymerization amplification. This DNAsensor consists of a long-tail probe, a short primer and polymerase. In the absence of the target, the long-tail probe and short primer are digested by Exo I, which minimizes the intercalation of fluorescence dye and reduces the background noise. In the presence of the target, the specific binding between the long-tail probe and the target triggers cycled polymerization reactions to form long dsDNA products. These long dsDNA products prevent effectively them from degrading by Exo I and amplify the fluorescence signals. In our sensing approach, the combination of the ExoI-assisted background reduction with the cycled polymerization amplification allows us to achieve a PCR-like sensitivity without labeling, washing separation and temperature cycles in a homogenous solution. Using total RNA samples extracted from hepatitis C virus (HCV) as targets, we further demonstrate the detection capability of the DNAsensor for complex nucleic acid samples, indicating its potential applicability for clinic molecular diagnostic assays., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
7. Long-tail probe-mediated cycled strand displacement amplification: label-free, isothermal and sensitive detection of nucleic acids.
- Author
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Su H, Long J, Guo Q, Meng X, Tan Y, Cai Q, Chen Z, and Meng X
- Subjects
- Binding Sites, DNA Probes chemical synthesis, DNA-Directed DNA Polymerase chemistry, Fluorescent Dyes, Hepacivirus chemistry, Inverted Repeat Sequences, Nucleic Acid Hybridization, Sensitivity and Specificity, Spectrometry, Fluorescence, Temperature, DNA chemistry, DNA Probes chemistry, Nucleic Acid Amplification Techniques methods, RNA, Viral analysis
- Abstract
We design a long-tail shaped DNA probe for the label-free, isothermal and sensitive detection of nucleic acids based on cycled strand displacement amplification (Ltail-CSDA). The long-tail probe, a stem-loop structure with a long poly(T) tail at 5'-termini, integrates target-binding and amplification and signaling within one multifunctional design. The specific binding between the long-tail probe and the target triggers a polymerization reaction, during which a long dsDNA product is synthesized and the hybridized target is displaced by the strand displacement activity of polymerase. The displaced target forms another specific probe-target binding and prompts cycled polymerization reactions. The proposed Ltail-CSDA has the distinct advantages of its isothermal nature, free-label, simplicity and attomolar sensitivity compared with other existing technologies. More significantly, the dynamic range of the method is extremely large, covering nine orders of magnitude. Using total RNA samples extracted from hepatitis C virus (HCV) as targets, we further demonstrate the detection capability of the method for complex nucleic acid samples, indicating its potential applicability for clinic molecular diagnostic assays., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
8. Amplified electrochemical DNA sensor using peroxidase-like DNAzyme.
- Author
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Guo Q, Bao Y, Yang X, Wang K, Wang Q, and Tan Y
- Subjects
- Catalysis, DNA genetics, G-Quadruplexes, Hydrogen Peroxide chemistry, Hydroquinones chemistry, Models, Genetic, Nucleic Acid Hybridization, Nucleic Acids chemistry, Oligonucleotides genetics, Time Factors, Biosensing Techniques methods, DNA chemistry, DNA, Catalytic chemistry, Electrochemistry methods
- Abstract
A novel electrochemical DNA sensor was developed here by using peroxidase-like G-quadruplex-based DNAzyme as a biocatalytic label. A hairpin structure including the G-quadruplex-based DNAzyme in a caged configuration and the target DNA probe were immobilized on Au-electrode surface. Upon hybridization with the target, the hairpin structure was opened, and the G-quadruplex-based DNAzyme was generated on the electrode surface, triggering the electrochemical oxidization of hydroquinone by H(2)O(2), which provide a quantitative measure for the detection of the target DNA. The DNA target was analyzed with a detection limit of 0.6 nM. This method is simple and easy to design without direct conjugation of redox-active element., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
9. Direct fluorescence detection of point mutations in human genomic DNA using microbead-based ligase chain reaction.
- Author
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Meng X, Yang X, Wang K, Guo Q, Tan Y, Mo Q, and Xu X
- Subjects
- DNA isolation & purification, Humans, Sensitivity and Specificity, beta-Thalassemia genetics, DNA genetics, Ligase Chain Reaction methods, Point Mutation, beta-Globins genetics
- Abstract
This report has described a convenient genotyping method capable of detecting point mutations directly in human genomic DNA based on the combination of ligase chain reaction (LCR) and microbead-enrichment technique. LCR primers, including a biotin-labeled common primer and two fluorescence-labeled allele-specific primers, are designed for two alleles of a mutated site. When genomic DNA carries the mutated site, the common primer and allele-specific primer are ligated to form exponential amplified biotin-labeled fluorescence ligation products. These ligated products are enriched by streptavidin-coated microbeads, and genotypes are identified conveniently according to the fluorescence color of microbeads using fluorescent microscopy. Due to amplification of LCR process and enrichment of microbeads, the detection limit of the proposed method is as low as 10(-15)mol/L templates. The method provides a convenient and simple strategy to detect point mutation directly in human genome. We have confirmed the efficiency of this approach with the identification of beta-globin gene point mutation, which results in the reduced production of globin in an inherited hemoglobin disorder thalassemia disease., (Copyright (c) 2009 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
10. Monitoring molecular beacon/DNA interactions using atomic force microscopy.
- Author
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Jin Y, Wang K, Tan W, Wu P, Wang Q, Huang H, Huang S, Tang Z, and Guo Q
- Subjects
- DNA Probes metabolism, Fluorescent Dyes, DNA metabolism, DNA ultrastructure, DNA Probes analysis, DNA Probes ultrastructure, Microscopy, Atomic Force
- Abstract
The molecular beacon (MB) is a new fluorescence probe containing a single-stranded oligonucleotide with a probe sequence embedded in complementary sequences that form a hairpin stem. Due to the inherent fluorescent signal transduction mechanism, an MB functions as a sensitive probe with a high signal-to-background ratio for real-time monitoring and provides a variety of exciting opportunities in DNA, RNA, and protein studies. To better understand the properties of MBs, the specific interactions between MB and target DNA (complementary and one-base mismatch) have been directly investigated by atomic force microscopy. The interaction force between a linear DNA probe and the target DNA was also detected and compared to that between MB and target DNA. The results demonstrate the high specificity of the MB/target DNA compared to the linear DNA/target DNA interaction.
- Published
- 2004
- Full Text
- View/download PDF
11. A DNA Aptamer Targeting Cellular Fibronectin Rather Than Plasma Fibronectin for Bioimaging and Targeted Chemotherapy of Tumors.
- Author
-
Zou, Yujian, Wang, Yufei, Wen, Xiaohong, Li, Chihao, Lei, Lei, Guo, Qiuping, Sun, Guoming, Yu, Li, and Nie, Hemin
- Subjects
FIBRONECTINS ,APTAMERS ,EPITHELIAL-mesenchymal transition ,DNA ,STROMAL cells ,STERIC hindrance ,CANCER chemotherapy - Abstract
Fibronectin (FN) is a well‐established hallmark of epithelial‐to‐mesenchymal transition, and may serve as an omnipresent cancer biomarker regardless of the origins of tumor cells. An ssDNA aptamer (ZY‐1) with highly selective binding affinity to mesenchymal stromal cells is previously developed, but the binding target of ZY‐1 on the cells and the underlying mechanism is yet to be understood. Here, the identification of FN as the target protein of aptamer ZY‐1 is reported for the first time and the mechanism of ZY‐1 binding to cFN is explored. The data indicate that ZY‐1 solely recognizes cellular fibronectin (cFN) rather than plasma fibronectin (pFN). The ZY‐1 binding to cFN is explored through computational modeling and the competition of heparin in binding cFN owing to steric hindrance is confirmed. The in vitro assay and noninvasive in vivo fluorescence imaging results validate the specificity of ZY‐1 in targeting cFN and sensitivity in detecting tumors. The ZY‐1‐mediated targeted cancer therapy using a proof‐of‐concept study with a ZY‐1‐based complex loaded with doxorubicin (Dox) is further proved. This study would facilitate more comprehensive studies of anti‐FN aptamers in the imaging and treatment of tumors and other FN‐associated diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
12. Cell-SELEX based selection and optimization of DNA aptamers for specific recognition of human cholangiocarcinoma QBC-939 cells.
- Author
-
Wan, Jun, Ye, Ling, Yang, Xiaohai, Guo, Qiuping, Wang, Kemin, Huang, Zhixiang, Tan, Yuyu, Yuan, Baoyin, and Xie, Qin
- Subjects
AMPLIFIED fragment length polymorphism ,DNA ,APTAMERS ,SYNTHETIC antibodies ,CHOLANGIOCARCINOMA ,BILE duct diseases ,CYTOLOGY - Abstract
Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy with no efficient early diagnosis and therapeutics available, so there is a call for effective molecular probes. Herein, we performed cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) to obtain aptamers for the specific recognition of human cholangiocarcinoma QBC-939 cells. By coordinating sequence homology analysis and secondary structure analysis, we successfully obtained two aptamers with dissociation constants (K
d ) in the low nanomolar range. A 23 nt truncated sequence was identified after further analysis on the secondary structure. More importantly, because hepatocellular carcinoma SMMC-7721 cells were employed as the control in the counter selection, the obtained aptamers demonstrated excellent specificity to the target cells, and no binding to several other hepatocellular carcinoma cell lines was observed. Moreover, the aptamers were initially found to recognize membrane proteins, giving them great potential in the field of biomarker discovery. These newly generated aptamers may play a key role in the early diagnosis and clinical treatment of CCA. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
13. Whole Cell-SELEX Aptamers for Highly Specific Fluorescence Molecular Imaging of Carcinomas In Vivo.
- Author
-
Shi, Hui, Cui, Wensi, He, Xiaoxiao, Guo, Qiuping, Wang, Kemin, Ye, Xiaosheng, and Tang, Jinlu
- Subjects
LUNG cancer diagnosis ,FLUORESCENCE ,CARCINOMA ,MOLECULAR probes ,CANCER cell culture ,CANCER cell differentiation ,FLOW cytometry - Abstract
Background: Carcinomas make up the majority of cancers. Their accurate and specific diagnoses are of great significance for the improvement of patients' curability. Methodology/Principal Findings: In this paper, we report an effectual example of the in vivo fluorescence molecular imaging of carcinomas with extremely high specificity based on whole cell-SELEX aptamers. Firstly, S6, an aptamer against A549 lung carcinoma cells, was adopted and labeled with Cy5 to serve as a molecular imaging probe. Flow cytometry assays revealed that Cy5-S6 could not only specifically label in vitro cultured A549 cells in buffer, but also successfully achieve the detection of ex vivo cultured target cells in serum. When applied to in vivo imaging, Cy5-S6 was demonstrated to possess high specificity in identifying A549 carcinoma through a systematic comparison investigation. Particularly, after Cy5-S6 was intravenously injected into nude mice which were simultaneously grafted with A549 lung carcinoma and Tca8113 tongue carcinoma, a much longer retention time of Cy5-S6 in A549 tumor was observed and a clear targeted cancer imaging result was presented. On this basis, to further promote the application to imaging other carcinomas, LS2 and ZY8, which are two aptamers selected by our group against Bel-7404 and SMMC-7721 liver carcinoma cells respectively, were tested in a similar way, both in vitro and in vivo. Results showed that these aptamers were even effective in differentiating liver carcinomas of different subtypes in the same body. Conclusions/Significance: This work might greatly advance the application of whole cell-SELEX aptamers to carcinomas-related in vivo researches. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
14. Novel protein detection method based on proximity-dependent polymerase reaction and aptamers.
- Author
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Yang Xiaohai, Wang Lei, Wang Kemin, Tan Weihong, Tang Hongxing, Meng Xiangxian, and Guo Qiuping
- Subjects
PROTEINS ,PROTEOMICS ,DNA ,THROMBIN ,FLUORESCENCE ,POLYMERASE chain reaction - Abstract
In recent years, specific detection of proteins is one of the hot issues about aptamers in proteomics. Here we reported a simple, sensitive and specific proximity-dependent protein assay with dual DNA aptamers. Thrombin was used as the model protein, and two aptamer probes with complementary Sequence at 3'-end were designed for the two distinct epitopes of the protein. Association of the two aptamers with thrombin resulted in stable hybrids due to the proximity of 3'-end, then polymerase reaction was induced. The amount of obtained dsDNA was indicated using the fluorescence dye Sybr Green I. The results showed that the initial velocity of polymerase reaction had a positive correlation with concentration of thrombin. The advantages of this dual-aptamer-based approach included simple and flexible design of aptamer probes, high selectivity and high sensitivity. The detection limit was 6.9 pmol/L. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
15. A DNA molecular diagnostic technology with LAMP-like sensitivity based on one pair of hairpin primers-mediated isothermal polymerization amplification.
- Author
-
Yin, Yao, Wu, Zuoci, Li, Guangping, Huang, Jin, Guo, Qiuping, and Meng, Xiangxian
- Subjects
- *
DNA primers , *NANOTECHNOLOGY , *BASE pairs , *DNA structure , *HAIRPIN (Genetics) , *DNA - Abstract
With the rapid development of isothermal amplification technology, DNA molecular diagnosis has become an important reference for clinical treatment. In this work, we have designed a DNA molecular diagnostic technology with LAMP-like sensitivity for nucleic acid analysis and detection based on only one pair of hairpin primers. This DNA molecular diagnostic technology consists of Bst DNA polymerase and one pair of hairpin primers, which are designed easily by adding a stem-loop structure to a target binding domain. When the target is present, the polymerization reaction between the hairpin primers and the target generates a specific dumbbell DNA similar to LAMP, which triggers cyclic amplification reactions to extend a series of long dsDNA products with repeated sequences by inserting fluorescent dye Eva Green observed the increase in fluorescence signal. In our method, using the hairpin primers-mediated isothermal polymerization amplification, we can specifically monitor 3–5 copies of the target nucleic acid in the system without labeling and temperature cycling in the reaction. In addition, serum samples from 13 patients with suspected schistosomiasis were targeted; we further demonstrated the ability of the technology to detect complex clinic samples, and its potentially inestimable applicability in clinic early molecular diagnostic research. In this work, we designed a simple DNA molecular diagnostic technology with LAMP-like sensitivity based on one pair of hairpin primers-mediated isothermal polymerization amplification. The hairpin primers are designed easily by adding a stem-loop structure to a target binding domain. By quickly forming a dumbbell DNA structure, which not only trigger product extension and polymerization, but also can achieve 3–5 copies of sensitivity, without the need to label and temperature cycling in the reaction. By inserting Eva Green to emit fluorescence, it can be used as a DNA molecular diagnostic sensor. Image 1 • The method can more easily form a dumbbell DNA structure. • Developed method can achieve a 3-5 copies sensitivity. • This design can form a DNA sensor by EVG to emit fluorescence. • The method can detect serum samples. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
16. Design and bioanalytical applications of DNA hairpin-based fluorescent probes.
- Author
-
Huang, Jin, Yang, Xiaohai, He, Xiaoxiao, Wang, Kemin, Liu, Jianbo, Shi, Hui, Wang, Qing, Guo, Qiuping, and He, Dinggeng
- Subjects
- *
FLUORESCENT probes , *HAIRPIN (Genetics) , *DNA , *CELLULAR signal transduction , *MOLECULAR probes , *FLUOROPHORES - Abstract
Highlights: [•] The advantages of DNA hairpin-based fluorescent probes (DHFPs) are discussed. [•] The various signal-labeled DHFP types and properties are listed. [•] The signal-transduction mechanisms are demonstrated by representative examples. [•] Some recent major bioanalytical applications of DHFPs are introduced. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
17. Whole Cell-SELEX Aptamers for Highly Specific Fluorescence Molecular Imaging of Carcinomas In Vivo.
- Author
-
Shi, Hui, Cui, Wensi, He, Xiaoxiao, Guo, Qiuping, Wang, Kemin, Ye, Xiaosheng, and Tang, Jinlu
- Subjects
- *
LUNG cancer diagnosis , *FLUORESCENCE , *CARCINOMA , *MOLECULAR probes , *CANCER cell culture , *CANCER cell differentiation , *FLOW cytometry - Abstract
Background: Carcinomas make up the majority of cancers. Their accurate and specific diagnoses are of great significance for the improvement of patients' curability. Methodology/Principal Findings: In this paper, we report an effectual example of the in vivo fluorescence molecular imaging of carcinomas with extremely high specificity based on whole cell-SELEX aptamers. Firstly, S6, an aptamer against A549 lung carcinoma cells, was adopted and labeled with Cy5 to serve as a molecular imaging probe. Flow cytometry assays revealed that Cy5-S6 could not only specifically label in vitro cultured A549 cells in buffer, but also successfully achieve the detection of ex vivo cultured target cells in serum. When applied to in vivo imaging, Cy5-S6 was demonstrated to possess high specificity in identifying A549 carcinoma through a systematic comparison investigation. Particularly, after Cy5-S6 was intravenously injected into nude mice which were simultaneously grafted with A549 lung carcinoma and Tca8113 tongue carcinoma, a much longer retention time of Cy5-S6 in A549 tumor was observed and a clear targeted cancer imaging result was presented. On this basis, to further promote the application to imaging other carcinomas, LS2 and ZY8, which are two aptamers selected by our group against Bel-7404 and SMMC-7721 liver carcinoma cells respectively, were tested in a similar way, both in vitro and in vivo. Results showed that these aptamers were even effective in differentiating liver carcinomas of different subtypes in the same body. Conclusions/Significance: This work might greatly advance the application of whole cell-SELEX aptamers to carcinomas-related in vivo researches. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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