1. Visualization of DNA and protein-DNA complexes with atomic force microscopy.
- Author
-
Lyubchenko YL, Gall AA, and Shlyakhtenko LS
- Subjects
- Aluminum Silicates chemistry, Bridged Bicyclo Compounds, Heterocyclic chemistry, Macromolecular Substances chemistry, Organosilicon Compounds chemistry, DNA chemistry, DNA-Binding Proteins chemistry, Microscopy, Atomic Force methods
- Abstract
This article describes sample preparation techniques for AFM imaging of DNA and protein-DNA complexes. The approach is based on chemical functionalization of the mica surface with aminopropyl silatrane (APS) to yield an APS-mica surface. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in the absence of divalent cations, and in a broad range of pH. The chapter describes the methodologies for the preparation of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein-DNA complexes.
- Published
- 2014
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