1. Cross-species chromosome painting.
- Author
-
Rens W, Fu B, O'Brien PC, and Ferguson-Smith M
- Subjects
- Animals, Cells, Cultured, Genomics methods, Species Specificity, Chromosome Painting methods, DNA analysis
- Abstract
Comparative genomics is an important and expanding field of research, and the genome-wide comparison of the chromosome constitution of different species makes a major contribution to this field. Cross-species chromosome painting is a powerful technique for establishing chromosome homology maps, defining the sites of chromosome fusions and fissions, investigating chromosome rearrangements during evolution and constructing ancestral karyotypes. Here the protocol for cross-species chromosome painting is presented. It includes sections on cell culture and metaphase preparation, labeling of chromosome-specific DNA, fluorescent in situ hybridization (chromosome painting) and image analysis. Cell culture and metaphase preparation can take between 1 and 2 wk depending on the cell culture. Labeling of chromosome-specific DNA is completed in 1 d. Fluorescent in situ hybridization can be completed in a maximum of 4 d.
- Published
- 2006
- Full Text
- View/download PDF