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Synthetic biologists use engineering principles to design and construct genetic circuits for programming cells with novel functions. A bottom-up approach is commonly used to design and construct genetic circuits by piecing together functional modules that are capable of reprogramming cells with novel behavior. While genetic circuits control cell operations through the tight regulation of gene expression, a diverse array of environmental factors within the extracellular space also has a significant impact on cell behavior. This extracellular space offers an addition route for synthetic biologists to apply their engineering principles to program cell-responsive modules within the extracellular space using biomaterials. In this review, we discuss how taking a bottom-up approach to build genetic circuits using DNA modules can be applied to biomaterials for controlling cell behavior from the extracellular milieu. We suggest that, by collectively controlling intrinsic and extrinsic signals in synthetic biology and biomaterials, tissue engineering outcomes can be improved. [ABSTRACT FROM AUTHOR]

Synthetic biology has emerged as an important technology for engineering cells to behave in controllable and predictable ways. The promise of this modern technology is dependent on our understanding of cellular complexity to allow us to engineer cells with novel function. In this regard, the fields of computer science and synthetic biology are critical for accelerating both our understanding of biological systems, and our ability to quantitatively engineer cells. Thus, advances in biology and biotechnology are arising at the intersection of computer science and synthetic biology approaches. This review seeks to introduce the field of synthetic biology to the computer science community, and to ignite a curiosity and interest in fostering a unique synergy for possible collaborations between synthetic biologists and computer scientists. [ABSTRACT FROM AUTHOR]

Summary: Here, we introduce an engineered, tunable genetic switch that couples repressor proteins and an RNAi target design to effectively turn any gene off. We used the switch to regulate the expression of EGFP in mouse and human cells and found that it offers >99% repression as well as the ability to tune gene expression. To demonstrate the system''s modularity and level of gene silencing, we used the switch to tightly regulate the expression of diphtheria toxin and Cre recombinase, respectively. We also used the switch to tune the expression of a proapoptotic gene and show that a threshold expression level is required to induce apoptosis. This work establishes a system for tight, tunable control of mammalian gene expression that can be used to explore the functional role of various genes as well as to determine whether a phenotype is the result of a threshold response to changes in gene expression. [Copyright &y& Elsevier]