1. Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA.
- Author
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Dezhampanah, Hamid, Darvishzad, Termeh, and Aghazadeh, Mehrnaz
- Subjects
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THERMODYNAMICS , *PHTHALOCYANINES , *BINDING sites , *ANIONS , *CALVES , *FLUORESCENCE spectroscopy , *DNA - Abstract
The interaction between anionic form of copper (II) N,N′,N″,N‴-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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