1. Extraction and electrochemical detection for quantification of trace-level DNA.
- Author
-
Cotchim S, Thavarungkul P, Kanatharana P, Thanakiatkrai P, Kitpipit T, and Limbut W
- Subjects
- Acrylic Resins chemistry, Adsorption, DNA chemistry, DNA isolation & purification, Electrochemical Techniques instrumentation, Electrodes, Humans, Limit of Detection, Magnetic Iron Oxide Nanoparticles chemistry, Methylene Blue chemistry, Mouth Mucosa chemistry, Propylamines chemistry, Reproducibility of Results, Silanes chemistry, Chemical Fractionation methods, DNA analysis, Electrochemical Techniques methods
- Abstract
A novel strategy was developed to extract, detect, and quantify trace-level DNA. For the extraction step, a composite of methylene blue (MB), poly(acrylic acid) (PAA), and modified iron oxide magnetic nanoparticles (IOMNPs) (PAA/IOMNPs) was used to adsorb DNA from the sample. MB-PAA/IOMNPs with adsorbed DNA were then separated from the solution with an external magnet and MB-DNA was eluted from PAA/IOMNPs with acetic acid. In the detection step, MB-DNA was adsorbed on the surface of 3-aminopropyltriethoxysilane (APTES)-modified glassy carbon electrode via electrostatic force. DNA was quantified by measuring the oxidation peak of MB at a potential -0.13 V vs. Ag/AgCl using differential pulse voltammetry. Under the optimal experimental conditions, the DNA sensor showed linear ranges from 0.001 to 0.005 pg μL
-1 , 0.005 to 0.070 pg μL-1 , and 0.070 to 0.400 pg μL-1 and a limit of detection of 0.87 fg μL-1 . The proposed sensor detected trace DNA in real samples with recoveries that ranged from 80.4 to 90.4%.- Published
- 2021
- Full Text
- View/download PDF