Search

Your search keyword '"Vianna EN"' showing total 1 results
Start Over Author "Vianna EN" Topic dna, protozoan

Refine your results

1 results on '"Vianna EN"'

1. An alternative method for DNA extraction and PCR identification of Entamoeba histolytica and E. dispar in fecal samples.

Author

Vianna EN, Costa JO, Santos CK, Cury MC, Silva EF, Costa AO, and Gomes MA

Subjects
Animals, DNA, Protozoan analysis, Entamoeba genetics, Entamoeba histolytica genetics, Humans, Reagent Kits, Diagnostic, Species Specificity, DNA, Protozoan isolation & purification, Entamoeba classification, Entamoeba histolytica classification, Entamoebiasis diagnosis, Entamoebiasis parasitology, Feces parasitology, Polymerase Chain Reaction methods
Abstract

Since it is known that Entamoeba dispar is non-pathogenic and morphologically similar to E. histolytica, there are many targets used in PCR for differentiating these species. However, obtaining high quality DNA from fecal samples is fundamental for PCR. Most methods are laborious or use kits that make diagnosis expensive. In the present work, a new simple, fast and cheap technique of DNA extraction from fecal samples was combined with a PCR for an episomal target in order to identify E. histolytica and E. dispar in feces.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results