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Start Over Author "Villela EV" Topic dna, helminth

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1. Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies.

Author

Martinez EM, Correia JA, Villela EV, Duarte AN, Ferreira LF, and Bello AR

Subjects
Animals, Base Sequence, DNA, Helminth genetics, Electrophoresis, Agar Gel, Genetic Markers, Humans, Molecular Sequence Data, Ovum, Random Amplified Polymorphic DNA Technique, DNA, Helminth isolation & purification, Trichuris genetics
Abstract

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing. T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.

Published
2003
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