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Morus nigra, black mulberry, is the most attractive among mulberry species. The study aimed to determine human health-promoting content and antifungal activity in fruits of seven black mulberry genotypes grown in the Tortum district of Turkey. The genotypes exhibited significant differences in most of the human health-promoting content. Among individual sugars and organic acids, glucose (6.98–8.03 g/100 g fresh weight base) and malic acid (6.33–10.45 g/100 g fresh weight base) were predominant. The ascorbic acid content of genotypes was found between 18.13 and 26.77 mg/100 g fresh weight base, indicating that black mulberry fruits had moderate ascorbic acid content. Total phenolic content, total antioxidant capacity, and total anthocyanin contents in fruit extracts of seven black mulberry genotypes were also investigated. The results showed that the levels of the aforementioned parameters changed depending on genotypes. The total phenolic and total anthocyanin contents were in the range of 1,656–2,348 μg gallic acid equivalent (GAE)/g and 623–804 μg C3GE (cyanidin-3-glucoside equivalent)/g, respectively. The genotypes had antioxidant capacity between 17.41 and 3.86 μg/mL and between 10.08 and 14.11 μmol TE/g measured by the 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assay, respectively. Some of the genotypes (TOR-1 and TOR-2) had high antifungal activity particularly against C. albicans. It was determined that the people living in the region traditionally use mulberries as blood enhancer, immune system booster, and mouth lesion treatments to protect themselves against different types of cancers and against inflammation. The present results confirm that attractive fruits of black mulberry are a rich natural source of phenolic antioxidants and can contribute to the dietary intake of antioxidants, depending on genotypes.

The cornelian cherry (Cornus mas L.) is considered to be one of those medicinal plants with important nutritional and therapeutic properties. The plant shows resistance against abiotic and biotic stressors in natural growing conditions and could be suitable to use in sustainable fruit production. This study was carried out on 22 local cornelian cherry (Cornus mas L.) genotypes, which were grown northwest of Bosnia and Herzegovina. Fruits of these 22 genotypes were harvested and analyzed during the ripening period in 2018/2019. Fruit weight, length, and width ranged from 1.38 to 3.01 g, 13.84 to 19.43 mm, and 10.92 to 14.79 mm, respectively. Dry matter content was determined to be the lowest at 11.67% and the highest at 21.89%. The genotypes had vitamin C content between 25.85 and 58.75 mg/100 g. Total phenolic and anthocyanin content were found to be quite variable among genotypes and ranged from 1240 to 6958 mg gallic acid equivalents (GAE) per 100 g fresh weight (FW) and 55.57 to 205.6 mg cyaniding-3-glucoside equivalents (CGE) per 100 g FW, respectively. The content of phosphorus and iron were between 155.52 to 263.06 mg per 100 g and 0.25 to 0.93 mg per 100 g, respectively. Principal Component Analysis (PCA) showed that the first and second components accounted for 44.05% and 60.50% of the total variance, and the major proportion of the first and second components were the morphometric properties and chemical traits of the cornelian cherry fruits. The results revealed that the characterized genotypes could be important for cornelian cherry breeders as ready crossing materials to obtain new cornelian cherry varieties and shows the potential of certain genotypes as a valuable source of natural antioxidants. The results may have served as a guide towards the development of sustainable production programs for cornelian cherries as well. [ABSTRACT FROM AUTHOR]

Progress in grape breeding requires the exploitation of genetic variation among market classes, races and gene pools. Wild grapevines (Vitis vinifera ssp. sylvestris) are being endangered in their natural habitats and high priorities should be given to the wild germplasm. Turkey is one of the richest sources of wild grapevine and they mostly grown on forest trees on river basin. The present study was carried out to determine the amount of genetic variation and the degree of relatedness among 23 wild grape genotypes using 17 simple-sequence-repeat markers (SSR). Two international grape cultivars, Cabernet Sauvignon and Merlot are also included study. Number of alleles per locus of the 17 Simple Sequence Repeat (SSR) markers ranged from 3.0 to 14.0 and a total of 162 alleles with an average of 9.53 alleles per locus. The average expected and observed heterozygosity values were 0.773 and 0.781, respectively, which exhibited high level of genetic diversity in the wild grape germplasm. The unweighted pair group method with arithmetic mean analysis revealed three main genetic clusters that partially separated wild grape genotypes each other and. The international cultivars formed a out group. The high genetic diversity among native wild grapes from Coruh valley is suggesting that this area could be one of the centre of diversity of the specie. The results indicate a substantial genetic diversity in V. vinifera ssp. sylvestris and the need of exploring a wider area to increase the chance of finding a particular genotype. [ABSTRACT FROM AUTHOR]

Disease and pest free chestnut (Castanea sativa Mill.) fruits were collected from 13 different seedling origin genotypes during the 2016 harvest season from the Mediterranean region of Turkey. The tree growth habit, nut weight, kernel ratio, kernel color, moisture, crude protein, crude fat, dietary fiber, total polyphenols, antioxidant activity, and fatty acid content of chestnuts were determined. The results showed that most of the genotypes had a semi-upright tree growth habit. The nut weight and kernel ratio were 5.87 g (A-9), 11.13 g (A-1), 73.38% (A-1), and 82.84% (A-12) among genotypes. The total crude fat content ranged from 0.80% (A-11) to 2.14% (A-12) while the crude protein content was between 4.78% (A-10) and 7.96% (A-9). Total polyphenols varied from 78 (A-12) to 124 (A-3) μg GAE/g and antioxidant activity was found to vary between 5.33 (A-12) and 9.83 (A-3) μmoles Trolox equivalent/g dry weight basis. Oleic and linoleic acid were the major fatty acids in all chestnut fruits, followed by palmitic and linolenic acids. The introduction of these genotypes as new cultivars by vegetative propagation may result in an increase in the quality of the chestnuts from the Mediterranean region of Turkey. [ABSTRACT FROM AUTHOR]

Some important morphological such as fruit weight, fruit external color, fruit shape, the number of berry per cluster, cluster color, plant crown habit, harvest date and biochemical characteristics including sugars, organic acids, total phenolics, total anthocyanins, and antioxidant capacity of thirteen wild grown Caucasian whortleberry sampled from Coruh valley, in northeastern Turkey, were determined. Antioxidant capacity was measured as FRAP assay (ferric reducing antioxidant power). Significantly differences were found among genotypes for most of the selected morphological and biochemical features. The genotypes had in general black fruit color and round shape. Harvest date varied from 2 August to 12 August. Fruit weight ranged from 0.37 to 1.08 g. Genotypes had only fructose and glucose in their fruits as sugars. Citric acid was dominant organic acids and was found between 8.87 and 11.20 g per kg of fresh fruits and followed by tartaric acid (2.85-3.30 g/kg). Fruits of genotypes exhibited very high total phenolic content, which ranged from 3740 to 5541 μg per g on a fresh weight basis. Total anthocyanin contents were between 81 and 172 μg of delphinidin-3-glucoside equivalent in per g fresh fruit indicating great diversity. [ABSTRACT FROM AUTHOR]

Some important physicochemical and bioactive characteristics of disease free 10 wild grown bilberry (Vaccinium myrtillus) accessions were evaluated. External and internal fruit quality was assessed by standard parameters (fruit weight, fruit color, fruit firmness, soluble solids, pH and total acidity) and bioactive contents (total phenolics, total anthocyanins, total antioxidant capacity and, vitamin C) in fruit were also determined. The commercial grown northern higbush blueberry, Vaccinium corymbosum cv. Bluecrop also included in the study to make comparision with bilberry samples. The highbush blueberry cv. Bluecrop had distinctive external fruit characteristics, such as bigger and more attractive fruits. However, the wild grown bilberry accessions showed interesting characters in mesocarp, such as high total phenolic content, total anthocyanin and total antioxidant capacity. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. Bluecrop while it was between 576–624 mg gallic acid equaivalent and 296–324 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of bilberry accessions. Moreover, wild accessions approximately had 2 folds higher antioxidant capacity than cv. Bluecrop. Results suggested that bilberry accessions may serve as a source of desirable genes to develop improved varieties that respond to the new needs of the market. [ABSTRACT FROM AUTHOR]

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The Rosa is one of the most diverse genera in the plant kingdom and, in particular, its fruits have been used for multiple purposes in different parts of the world for centuries. Within the genus, Rosa canina and Rosa dumalis are, economically, the most important species and dominate Rosa fruit production. In this study, some important fruit and shrub traits of ten Rosa canina and ten Rosa dumalis ecotypes collected from rural areas of Kars province, located in the east Anatolia region of Turkey were investigated. We found significant differences among ecotypes in most of the morphological and biochemical traits. The ecotypes were found between 1446–2210 m altitude. Fruit weight and fruit flesh ratio ranged from 2.95 g to 4.72 g and 62.55% to 74.42%, respectively. SSC (Soluble Solid Content), Vitamin C, total phenolic, total flavonoid, total carotenoid, and total anthocyanin content of the ecotypes ranged from 16.9–22.7%, 430–690 mg per 100 g FW (fresh weight), 390–532 mg gallic acid equivalent per 100 g FW, 0.88–2.04 mg per g FW, 6.83–15.17 mg per g FW and 3.62–7.81 mg cyanidin-3-glucoside equivalent per kg, respectively. Antioxidant activity was determined to be between 19.7–34.7 mg ascorbic acid equivalent per g fresh weight. Rosa ecotypes contained chlorogenic acid and rutin the most as phenolic compound. Our results indicated great diversity within both R. canina and R. dumalis fruits. [ABSTRACT FROM AUTHOR]

Wild edible fruits have been used centuries by local people mainly for nutrition purposes and they have attracted the attention of researchers in the last decade throughout the world. Among wild edible fruits, the strawberry tree (Arbutus unedo) is widely distributed mainly in Mediterranean countries. However, the lack of information about strawberry tree genetic resources in Mediterranean countries is a major cause of its low exploitation by breeders. For a practical use and exploitation of the strawberry tree native germplasm, phenological, morphological, and biochemical characterization of the plant is essential. In this study, some important phenological, morphological, and biochemical characteristics of sixteen local strawberry tree genotypes were determined. Significant variations (p < 0.05) were observed in most of the investigated phenological, morphological, and biochemical traits of the genotypes. The genotypes were harvested between 2–21 November. The fruit weight and the number of fruits per cluster of the genotypes varied from 3.89 to 6.86 g and 3.61 to 7.94, respectively. Vitamin C and total dietary fiber contents of genotypes were found quite variable, ranging from 58 to 93 mg/100 g and 7.04 to 12.36 g/100 g, respectively. The fruits of the strawberry tree include mainly fructose and glucose rather than sucrose. Local strawberry tree genotypes are found to be promising for further valorization through horticultural characteristics for breeding. Some genotypes had significantly higher fruit weight, vitamin C concentrations, total dietary fiber, and total phenolic concentration and may be good selections for producing health products. [ABSTRACT FROM AUTHOR]

In the present study, fruit weight, fruit firmness, external fruit color, soluble solid content, pH, titratable acidity, vitamin C, total phenolics, total anthocyanins and antioxidant capacity of eight wild bilberry grown in Coruh valley in northeastern Turkey were determined. The blueberry cv. 'Bluecrop' was also studied to make comparison with bilberry genotypes. Antioxidant activity of bilberry and blueberry fruits were comparatively assessed by FRAP (Ferric reducing antioxidant power) assay. Significantly higher contents of phenolics and anthocyanins were found in bilberry fruits comparted with blueberry fruits. However, blueberry cv. 'Bluecrop' exhibited higher fruit weight and vitamin C content than bilberry fruits. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. 'Bluecrop', while it was between 492-563 mg gallic acid equaivalent and 307-342 mg of cyanidin-3- glucoside equivalent in 100 g fresh fruits of bilberry accessions. Moreover, wild accessions had approximately 2-3 folds higher antioxidant capacity than cv. 'Bluecrop'. The obtained results suggest the possibility of improving the bioactive and antioxidant properties of bilberry cultivars by using wild ones in cross breedings. It was also concluded that genotypes significantly affect the bioactive content of fruits and consequently the possibility of using wild bilberry fruits as a potential source of natural antioxidants in food industry is worth to be considered. [ABSTRACT FROM AUTHOR]

Turkey ranked third place in the world for chestnut production after China and Bolivia and the country has unique chestnut populations including valuable and diverse seed propagated chestnut genotypes. In this study, chestnuts (Castanea sativa) were collected during the 2016 harvest season from Northeastern part of Turkey from promising 12 different seedling origin genotypes. Tree growth habit, nut weight, kernel ratio, kernel color, moisture, crude protein, crude fat, dietary fiber, total polyphenols, antioxidant activity and fatty acid content of fruits belongs to 12 genotypes were determined. The results showed that, the majority of genotypes had semi upright tree growth habit. The nut weight and kernel ratio were between 5.05 g (K-10) and 10.10 g (K-5) and 71.10% (K-1) and 82.44% (K-3) among genotypes. The total crude fat content ranged from 0.87% (K-7) to 2.61% (K-1) while the crude protein ranged from 4.80% (K-7) to 7.65% (K-1). The dietary fiber content made up no more than 3.61% of the remaining portion of the kernel. It was found that total polyphenols was between 1.66 and 2.70 g GAE/kg and antioxidant activity was between 5.80 and 9.07 µmol Trolox equivalent/g dry weight basis. Oleic and linoleic acid were the major fatty acids in all chestnut fruits and followed by palmitic and linolenic acid. The results showed that there was enough variations among seed propagated chestnut genotypes for most of the searched parameters and this highlights the importance of conserving the genotypes, as their high levels of heterogeneity allow them to respond to abiotic and biotic stresses and adapt to low-input farming systems. [ABSTRACT FROM AUTHOR]

Wild edible fruits have been gaining much interest more recently because of their better biochemical content and widely use in ethno medicine treating common disease such as cold, fever and other medicinal claims are now supported with sound scientific evidences. In this study, diversity of some important physicochemical characteristics (plant growth habit, fruit shape, fruit color, fruit weight, pulp ratio, soluble solid content, total phenolics, total anthocyanin, antioxidant activity, sugars, organic acids) of fruits from fourteen promising barberry (Berberis vulgaris L.) selections grown in Erzurum province in Turkey were investigated. Significant differences were observed between the studied genotypes for most of the physicochemical parameters. Fruit weight and pulp ratio were found between 0.102 (25ERZ5) and 0.342 g (25ERZ7) and 60.81% (25ERZ2) and 75.41% (25ERZ11). Total phenolic and anthocyanin content ranged from 2281 (25ERZ5) to 3462 (25ERZ7) as mg GAE per liter fruit juice and 360 to 874 mg as cyanidin-3-glucoside per liter fruit juice. Glucose and fructose were found to be dominant sugars in all barberry accessions analyzed. [ABSTRACT FROM AUTHOR]

Abstract: Carob (Ceratonia siliqua L.) is natively grown in the coastal areas of the Aegean and Mediterranean region in Turkey. The main carob growing areas (Mediterranean and Aegean) were surveyed and a total 70 promising wild and grafted carob genotypes were selected based on physical and chemical pod properties. Preliminary results showed that Turkey has very rich carob genetic resources containing considerable variations for most of the pod traits. In general, wild carob had smaller pods than the grafted ones. Pod mass ranged from 29.16g to 120.28g in the wild genotypes and 71.71g to 147.91g in the grafted genotypes with an average 107.61g for the grafted and 63.72g for the wild genotypes. The average pod dimensions (width, length and thickness) were 18.16mm, 16.18cm and 6.42mm for the wild genotypes and 21.79mm, 16.91cm and 8.79mm for the grafted genotypes. The average soluble solid content ranged from 59.42% in the wild and 64.06% in the grafted genotypes. The average total acidity of the wild and grafted genotypes was similar (0.78%). The pod traits of the wild genotypes differ significantly from those of the grafted genotypes, and the wild genotypes showed higher variations in these traits compared to the grafted genotypes. [Copyright &y& Elsevier]

• A number of European Cranberrybush studied first time. • Genotypes showed distinct biochemical features which important for human health and food processing. • Some of the wild grown genotypes were identified with distinct traits making their potential a cultivar candidate or as parent for genetic improvement. • Yield per bush of genotypes were found close to international standard cultivars. In this study, a comprehensive morphological and biochemical characterization of 14 European Cranberrybush (Viburnum opulus L.) genotypes that were collected from Gumushane province of Turkey is provided. Additionally, their potentials as cultivar candidate, natural antioxidants, colorants and ingredients were exploited. Yield per bush, fruit weight, number of fruits per raceme and number of racemes per bush were ranged from 4.7 kg to 6.2 kg; 0.53 to 0.86 g; 34.4–53.1 and 119–307 among genotypes indicating high diversity. The majority of genotypes (8) had red fruit skin color and rest of the genotypes had equally light red and dark red fruit skin color. European Cranberrybush fruits had medium level vitamin C content (28−43 mg per 100 g fresh fruit) and the genotypes had SSC (Soluble Solid Content) between 10.40 and 12.20 %. Crude protein and crude cellulose of the genotypes varied from 5.67 to 6.71 (%) and 16.6–18.7 (%), respectively. Total phenol and total anthocyanin content ranged from 696 to 830 mg gallic acid equivalent per 100 g fresh weight and 27.6–54.3 mg per 100 g fresh fruit expressed cyanidin-3-glucoside. Antioxidant activity were found between 27.67 and 35.65 μmol trolox equivalents per g fresh fruit based on FRAP (Ferric Reducing Ability of Plasma) assay. Total lipid content ranged from 10.3–13.3 (%) and oleic acid was dominant fatty acids for all genotypes (57.3–70.4). Local genotypes are found to be promising for further valorization through horticultural characteristics and novel antioxidants, colorants and ingredients. [ABSTRACT FROM AUTHOR]