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Your search keyword '"Travis, E.R."' showing total 1 results
Start Over Author "Travis, E.R." Topic direct repeat
1 results on '"Travis, E.R."'

1. LAMBDR: Long-range amplification and Nanopore sequencing of theMycobacterium bovis direct-repeat region. A novel method for in-silico spoligotyping ofM. bovisdirectly from badger faeces

Author

James, R.S., Travis, E.R., Millard, A. D., Hewlett, P.C., Kravar-Garde, L., and Wellington, E.M.

Subjects
Genetics, Mycobacterium bovis, Sequence analysis, law, In silico, Direct repeat, Nanopore sequencing, Biology, Primer (molecular biology), biology.organism_classification, Genome, Polymerase chain reaction, law.invention
Abstract

The environment is an overlooked source ofMycobacterium bovis, the causative agent of bovine TB. Long read, end to end sequencing of variable repeat regions across theM. bovisgenome was evaluated as a method of acquiring rapid strain level resolution directly from environmental samples. Eight samples ofM. bovis, two BCG strains (Danish and Pasteur), and a singleM. tuberculosistype culture (NCTC 13144) were used to generate data for this method. Long range PCR amplification of the direct repeat region was used to synthesize ∼5kb template DNA for onward sequence analysis. This has permitted culture independent identification ofM. bovisspoligotypes present in the environment. Sequence level analysis of the direct repeat region showed that spoligotyping may underestimate strain diversity due to the inability to identify both SNPs and primer binding mutations using a biotinylated hybridisation approach.

Published
2019

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