1. Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection
- Author
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Frankenfeld, Celeste N., Rosenbaugh, Matt R., Fogarty, Barbara A., and Lunte, Susan M.
- Subjects
- *
NITRITES , *NITRATES , *ELECTROKINETICS , *GEL electrophoresis - Abstract
Abstract: A method for the separation and direct detection of peroxynitrite (ONOO−) and two of its degradation products, nitrite (NO2 −) and nitrate (NO3 −), using capillary electrophoresis with ultraviolet detection is described. The separation parameters were optimized and included electrokinetic injection, a run buffer consisting of 25mM K2HPO4 7.5mM DTAB, pH 12, and a field strength of −323V/cm. A diode array UV detector was employed in these studies as it allowed the determination of all three species simultaneously. Nitrate and nitrite provided the maximum response at 214nm while peroxynitrite generated the best response at 302nm. All three species could be detected at 214nm, while simultaneous detection at 214 and 302nm positively identified each peak. [Copyright &y& Elsevier]
- Published
- 2006
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