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The present study examined the effects of acute nitrate (NO 3 - ) supplementation ingested in the form of concentrated beetroot juice on cross-country roller-ski performance in normoxia (N) and normobaric hypoxia (H). Eight competitive cross-country skiers (five males: age 22 ± 3 years, V·O 2max 71.5 ± 4.7 mL kg -1 ·min -1 ; three females: age 21 ± 1 years, V·O 2max 58.4 ± 2.5 mL kg -1 ·min -1 ) were supplemented with a single dose of NO 3 - -rich beetroot juice (BRJ, ∼13 mmol NO 3 - ) or a NO 3 - -depleted placebo (PL, ∼0 mmol NO 3 - ) and performed 2 x 6-min submaximal exercise bouts and a 1000-m time-trial (TT) on a treadmill in N (20.9% O 2 ) or H (16.8% O 2 ). The four experimental trials were presented in a randomised, counter-balanced order. Plasma NO 3 - and nitrite concentrations were significantly higher following BRJ compared to PL (both p < 0.001). However, respiratory variables, heart rate, blood lactate concentration, ratings of perceived exertion, and near-infrared spectroscopy-derived measures of muscle tissue oxygenation during submaximal exercise were not significantly different between BRJ and PL (all p > 0.05). Likewise, time to complete the TT was unaffected by supplementation in both N and H (p > 0.05). In conclusion, an acute dose of ∼13 mmol NO 3 - does not affect physiological or performance responses to submaximal or maximal treadmill roller-skiing in competitive cross-country skiers exercising in N and H., (Copyright © 2017 Elsevier Inc. All rights reserved.)

Exposure to altitude results in multiple physiological consequences. These include, but are not limited to, a reduced maximal oxygen consumption, drop in arterial oxygen saturation, and increase in muscle metabolic perturbations at a fixed sub-maximal work rate. Exercise capacity during fixed work rate or incremental exercise and time-trial performance are also impaired at altitude relative to sea level. Recently, dietary nitrate (NO 3 - ) supplementation has attracted considerable interest as a nutritional aid during altitude exposure. In this review, we summarise and critically evaluate the physiological and performance effects of dietary NO 3 - supplementation during exposure to simulated and terrestrial altitude. Previous investigations at simulated altitude indicate that NO 3 - supplementation may reduce the oxygen cost of exercise, elevate arterial and tissue oxygen saturation, improve muscle metabolic function, and enhance exercise capacity/performance. Conversely, current evidence suggests that NO 3 - supplementation does not augment the training response at simulated altitude. Few studies have evaluated the effects of NO 3 - at terrestrial altitude. Current evidence indicates potential improvements in endothelial function at terrestrial altitude following NO 3 - supplementation. No effects of NO 3 - supplementation have been observed on oxygen consumption or arterial oxygen saturation at terrestrial altitude, although further research is warranted. Limitations of the present body of literature are discussed, and directions for future research are provided.

Introduction: Dietary inorganic nitrate is a popular nutritional supplement, which increases nitric oxide bioavailability and may improve exercise performance. Despite over a decade of research into the effects of dietary nitrate supplementation during exercise there is currently no expert consensus on how, when and for whom this compound could be recommended as an ergogenic aid. Moreover, there is no consensus on the safe administration of dietary nitrate as an ergogenic aid. This study aimed to address these research gaps. Methods: The modified Delphi technique was used to establish the views of 12 expert panel members on the use of dietary nitrate as an ergogenic aid. Over three iterative rounds (two via questionnaire and one via videoconferencing), the expert panel members voted on 222 statements relating to dietary nitrate as an ergogenic aid. Consensus was reached when > 80% of the panel provided the same answer (i.e. yes or no). Statements for which > 80% of the panel cast a vote of insufficient evidence were categorised as such and removed from further voting. These statements were subsequently used to identify directions for future research. Results: The 12 panel members contributed to voting in all three rounds. A total of 39 statements (17.6%) reached consensus across the three rounds (20 yes, 19 no). In round one, 21 statements reached consensus (11 yes, 10 no). In round two, seven further statements reached consensus (4 yes, 3 no). In round three, an additional 11 statements reached consensus (5 yes, 6 no). The panel agreed that there was insufficient evidence for 134 (60.4%) of the statements, and were unable to agree on the outcome of the remaining statements. Conclusions: This study provides information on the current expert consensus on dietary nitrate, which may be of value to athletes, coaches, practitioners and researchers. The effects of dietary nitrate appear to be diminished in individuals with a higher aerobic fitness (peak oxygen consumption [V̇O2peak] > 60 ml/kg/min), and therefore, aerobic fitness should be taken into account when considering use of dietary nitrate as an ergogenic aid. It is recommended that athletes looking to benefit from dietary nitrate supplementation should consume 8–16 mmol nitrate acutely or 4–16 mmol/day nitrate chronically (with the final dose ingested 2–4 h pre-exercise) to maximise ergogenic effects, taking into consideration that, from a safety perspective, athletes may be best advised to increase their intake of nitrate via vegetables and vegetable juices. Acute nitrate supplementation up to ~ 16 mmol is believed to be safe, although the safety of chronic nitrate supplementation requires further investigation. The expert panel agreed that there was insufficient evidence for most of the appraised statements, highlighting the need for future research in this area. [ABSTRACT FROM AUTHOR]

Dietary supplements such as caffeine and beetroot juice are used by athletes in an attempt to optimize performance and therefore gain an advantage in competition. The aim of this study was to investigate the individual and combined effects of caffeine and beetroot-juice supplementation during submaximal and maximal treadmill running. Seven males (maximal oxygen uptake: 59.0 ± 2.9 mL·kg-1·min-1) and 2 females (maximal oxygen uptake: 53.1 ± 11.4 mL·kg-1·min-1) performed a preliminary trial followed by 4 experimental test sessions. Each test session consisted of two 5-min submaximal running bouts (at ∼70% and 80% of maximal oxygen uptake) and a maximal 1-km time trial (TT) in a laboratory. Participants ingested 70 mL of concentrated beetroot juice containing either 7.3 mmol of nitrate (BR) or no nitrate (PBR) 2.5 h prior to each test session, then either caffeine (C) at 4.8 ± 0.4 (4.3-5.6) mg/kg of body mass or a caffeine placebo (PC) 45 min before each test session. The 4 test sessions (BR-C, BR-PC, PBR-C, and PBR-PC) were presented in a counterbalanced and double-blind manner. No significant differences were identified between the 4 interventions regarding relative oxygen uptake, running economy, respiratory exchange ratio, heart rate (HR), or rating of perceived exertion (RPE) at the 2 submaximal intensities ( P > 0.05). Moreover, there were no significant differences in performance, maximum HR, peak blood lactate concentration, or RPE during the maximal TT when comparing the interventions ( P > 0.05). In conclusion, no beneficial effects of supplementing with typical doses of caffeine, beetroot juice, or a combination of the two were observed for physiological, perceptual, or performance responses during submaximal or maximal treadmill running exercise. [ABSTRACT FROM AUTHOR]