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A colorimetric method for the analysis of dolutegravir in pure form and in tablets has been developed based on the formation of green colour complex. The method is based on the diazotization of carbonyl group and coupling with 3-methyl-2-benzothiazolinone hydrazone reagent in presence of ferric chloride to form green colour complex, by reaction of NH2 (amine) group present in the 3-methyl-2-benzothiazolinone hydrazone reagent with the carbonyl functional group of dolutegravir by eliminating one water molecule. The complex exhibited absorption maxima at 632 nm obeying Beer's law in range of 10-18 pg mL-1. This method is simple, precise and accurate with recovery of 99.8-100 %. The line equation Y = 0.0082x + 0.0292 with correlation coefficient (r2) of 0.991 was obtained. [ABSTRACT FROM AUTHOR]