1. Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China.
- Author
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Liu BJ, Zuo YZ, Gu WY, Luo SX, Shi QK, Hou LS, Zhong F, and Fan JH
- Subjects
- Animals, China epidemiology, Coronavirus Infections epidemiology, Coronavirus Infections virology, Diarrhea epidemiology, Diarrhea virology, Evolution, Molecular, Feces virology, Genes, Viral genetics, Genomics, Intestines virology, Phylogeny, Swine, Swine Diseases epidemiology, Coronavirus genetics, Coronavirus isolation & purification, Coronavirus Infections veterinary, Diarrhea veterinary, Swine Diseases virology
- Abstract
Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB-BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB-BD strain was sequenced and analysed. Genomic analysis showed that the HB-BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains., (© 2018 Blackwell Verlag GmbH.)
- Published
- 2018
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