1. Under‐5‐Minute Immunoblot Assays by Vortex Fluidic Device Acceleration
- Author
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Sanders, Emily C, Sen, Sanjana R, Gelston, Aidan A, Santos, Alicia M, Luo, Xuan, Bhuvan, Keertna, Tang, Derek Y, Raston, Colin L, and Weiss, Gregory A
- Subjects
Chemical Sciences ,Biotechnology ,Bioengineering ,Precision Medicine ,4.1 Discovery and preclinical testing of markers and technologies ,Good Health and Well Being ,Acceleration ,Humans ,Immunoassay ,Microfluidics ,Proteomics ,Reproducibility of Results ,Diagnostics ,Vortex Fluidics ,Organic Chemistry ,Chemical sciences - Abstract
Unlocking the potential of personalized medicine in point-of-care settings requires a new generation of biomarker and proteomic assays. Ideally, assays could inexpensively perform hundreds of quantitative protein measurements in parallel at the bedsides of patients. This goal greatly exceeds current capabilities. Furthermore, biomarker assays are often challenging to translate from benchtop to clinic due to difficulties achieving and assessing the necessary selectivity, sensitivity, and reproducibility. To address these challenges, we developed an efficient (70 %) immunoassay method. Specifically, the immunoblot membrane is dotted with the sample and then developed in a vortex fluidic device (VFD) reactor. All assay steps-blocking, binding, and washing-leverage the unique thin-film microfluidics of the VFD. The approach can accelerate direct, indirect, and sandwich immunoblot assays. The applications demonstrated include assays relevant to both the laboratory and the clinic.
- Published
- 2022