13 results on '"Demar, Magalie"'
Search Results
2. Accuracy of SD Malaria Ag P.f/Pan® as a rapid diagnostic test in French Amazonia.
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Pujo, Jean Marc, Houcke, Stéphanie, Lemmonier, Sarah, Portecop, Patrick, Frémery, Alexis, Blanchet, Denis, Djossou, Felix, Kallel, Hatem, and Demar, Magalie
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MALARIA ,DIAGNOSIS methods ,DIAGNOSIS ,BLOOD testing ,PLASMODIUM vivax - Abstract
Background: French Guiana (FG) is a French overseas territory where malaria is endemic. The current incidence rate is 0.74‰ inhabitants, and Plasmodium vivax is widely predominating even though Plasmodium falciparum is still present due to imported cases mainly from Africa. In FG, rapid diagnostic test (SD Malaria Ag P.f/Pan®) is based on the detection of pan-pLDH, PfHRP2, and PfHRP3 antigens, while in South America, the share of deletion of PfHRP2 gene is significantly increasing. Accordingly, the study questions the reliability of RDTs in the Amazonian context. Methods: The study is retrospective. It is conducted over 4 years and analysed 12,880 rapid diagnostic tests (RDTs) compared to concomitant Blood Film Tests (BFTs) sampled for malaria diagnosis. Results: The global assessment of the accuracy of SD Malaria Ag P.f/Pan® in the diagnostic of malaria shows both Positive and Negative Predictive Values (PPV and NPV) higher than 95%, except for PPV in the diagnosis of malaria to P. falciparum (88%). Overall, the concordance rate between RDT and BFT (positive/positive; negative/negative) was 99.5%. The PPV of the RDT in the follow-up of patients diagnosed with P. falciparum was the lowest during the first 28 days. The PPV of the RDT in the follow-up of patients diagnosed with P. vivax was the lowest during the first 21 days. The global sensitivity of SD Malaria Ag P.f/Pan® test was, on average, 96% (88.2–100) for P. falciparum and 93% (90.6–94.2) for P. vivax. The global specificity was 99.8% (99.5–100) for all included species. Conclusion: SD Malaria Ag P.f/Pan® is a reliable rapid test used for the first-line diagnosis in remote healthcare centres. The test results should be interpreted in the light of patient's recent medical history and the date of arrival to FG. [ABSTRACT FROM AUTHOR]
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- 2021
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3. Cytological and Histopathological Spectrum of Histoplasmosis: 15 Years of Experience in French Guiana.
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Drak Alsibai, Kinan, Couppié, Pierre, Blanchet, Denis, Adenis, Antoine, Epelboin, Loïc, Blaizot, Romain, Louvel, Dominique, Djossou, Félix, Demar, Magalie, and Nacher, Mathieu
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HISTOPLASMOSIS ,SMALL intestine ,ALIMENTARY canal ,COLON (Anatomy) ,BONE marrow - Abstract
Background: Disseminated histoplasmosis remains a major killer of immunocompromised patients in Latin America. Cytological and histological methods are usually present in most hospitals and may represent a precious diagnostic method. We report 15 years of experience of the department of pathology of the Centre Hospitalier de Cayenne Andrée Rosemon in French Guiana. Methods: Specimens from live patients from January 2005 to June 2020 with the presence of H. capsulatum on cytological and/or histological analysis were analyzed. All specimens were examined by an experienced pathologist. The analysis was descriptive. Results: Two hundred two cytological and histological samples were diagnosed with histoplasmosis between January 2005 and June 2020. The 202 samples included 153 (75.7%) histopathological formalin-fixed and paraffin-embedded tissues (biopsy or surgical specimens) and 49 (24.3%) cytological analysis from all organs. One hundred thirty-four patients (82.7%) were HIV-positive, 15 patients (9.3%) had immunosuppressant treatment, and 13 patients (8%) were immunocompetent. Seventy-eight of 202 (38.5%) were samples from the digestive tract, mostly the colon (53/78 cases, 70%) and small intestine (14/78 cases, 18%). Microorganisms were more numerous in digestive samples (notably the colon) than in other organs. Lymphocyte and histiocyte inflammation of moderate to marked intensity were observed in all positive specimens. Tuberculoid epithelioid granuloma were present in 16/78 (20,5%) specimens including 14 colon and 2 small intestine specimens. There were 11/202 cases of liver histoplasmosis, 26/202 (12,8%) cases of pulmonary histoplasmosis. Bone marrow involvement was diagnosed in 14 (2%) specimens (8 aspiration and 6 biopsies). Lymph nodes were positive in 42 specimens (31 histology and 11 cytology). Histopathological analysis of the 31 lymph nodes showed a variable histological appearance. Tuberculoid forms were most frequent (24/31, 77,4%). Conclusions: From the pathologist perspective, this is the largest series to date showing that digestive involvement was the most frequent, usually with a tuberculoid form and a greater load of Histoplasma. With awareness and expertise, cytology and pathology are widely available methods that can give life-saving results in a short time to help orient clinicians facing a potentially fatal infection requiring prompt treatment. [ABSTRACT FROM AUTHOR]
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- 2020
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4. Heterogeneity of Clinical Presentations and Paraclinical Explorations to Diagnose Disseminated Histoplasmosis in Patients with Advanced HIV: 34 Years of Experience in French Guiana.
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Nacher, Mathieu, Valdes, Audrey, Adenis, Antoine, Blaizot, Romain, Abboud, Philippe, Demar, Magalie, Djossou, Félix, Epelboin, Loïc, Alsibai, Kinan Drak, Misslin, Caroline, Ntab, Balthazar, and Couppié, Pierre
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HISTOPLASMOSIS ,MULTIPLE correspondence analysis (Statistics) ,EIGENVALUES - Abstract
We aimed to describe the ways patients with disseminated histoplasmosis—a multifaceted and often lethal disease—present themselves and are explored. A retrospective, observational, multicentric study spanned the period between 1 January 1981 and 1 October 2014. Principal component analysis was performed for the sampling sites and for the clinical signs and symptoms. The factor loadings of the principal components were selected for eigenvalues > 1. The most frequent signs and symptoms were an alteration of the WHO general performance status, fever, digestive tract, respiratory signs and symptoms and lymphadenopathies. The most common sites sampled were bone marrow, respiratory tract, blood, lymph node and liver biopsies, with significant variations in the number of sites from which samples were taken to try to identify the pathogen. The principal component analysis clinical signs and symptoms leading to the diagnosis showed four main lines of variation. The factor loadings of the four main components were compatible with four broad types of clinical presentations and four types of exploration strategies. Extracting simple algorithms was difficult, emphasizing the importance of clinical expertise when diagnosis depends on obtaining a sample where Histoplasma can be seen or grown. Histoplasma antigen detection tests will help simplifying the algorithms. [ABSTRACT FROM AUTHOR]
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- 2020
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5. First description of bullous lupus associated with cutaneous leishmaniasis: coincidence or trigger?
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Bertin, Chloé, Drak Alsibai, Kinan, Demar, Magalie, Couppié, Pierre, and Blaizot, Romain
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CUTANEOUS leishmaniasis ,BULLOUS pemphigoid ,EPIDERMOLYSIS bullosa ,PROTOZOAN diseases ,COINCIDENCE ,DIAGNOSIS ,PARASITIC diseases - Abstract
American cutaneous leishmaniasis is endemic in French Guiana with an annual incidence rate estimated between 15 and 20 new cases per 10,000 inhabitants.1 To our knowledge, the association of cutaneous leishmaniasis with bullous lupus has never been described so far. (b) Hematoxylin and eosin stain (×600) of a cutaneous biopsy showing a blister lesion with dermal-epidermal cleavage, focal keratinocyte necrosis, and perivascular lymphocyte-dominant inflammatory infiltrate gl A bullous lupus associated with cutaneous leishmaniasis was diagnosed. [Extracted from the article]
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- 2021
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6. A complementary tool for management of disseminated Histoplasma capsulatum var. capsulatum infections in AIDS patients
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Iriart, Xavier, Blanchet, Denis, Ménard, Sandie, Lavergne, Rose-Anne, Chauvin, Pamela, Adenis, Antoine, Cassaing, Sophie, Fillaux, Judith, Magnaval, Jean-François, Demar, Magalie Pierre, Carme, Bernard, Bessieres, Marie-Hélène, Couppié, Pierre, Nacher, Mathieu, Berry, Antoine, Aznar, Christine, Service de Parasitologie et Mycologie, CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Centre d'investigation clinique Antilles-Guyane (CIC - Antilles Guyane), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pointe-à-Pitre/Abymes [Guadeloupe] -CHU de la Martinique [Fort de France]-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Epidémiologie des parasitoses et mycoses tropicales, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles et de la Guyane (UAG), Service de Parasitologie et Mycologie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), and Université des Antilles et de la Guyane (UAG)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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AIDS ,reactivity ,cross ,histoplasmosis ,galactomannan ,antibody ,parasitic diseases ,Diagnosis ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,bacterial infections and mycoses ,Platelia ,Histoplasma capsulatum - Abstract
International audience; In South America, disseminated histoplasmosis due to Histoplasma capsulatum var. capsulatum (H. capsulatum), is a severe and frequent opportunistic infection in AIDS patients. In areas outside the USA where specific-Histoplasma antigen detection is not available, the diagnosis is difficult. With the galactomannan antigen (GM) detection, a test commonly used for invasive aspergillosis diagnosis, there is a cross-reactivity with H. capsulatum that can be helpful for the diagnosis of histoplasmosis. The aim of this study was to evaluate the GM detection for the diagnosis of disseminated histoplasmosis in AIDS patients. The performance of the GM detection was evaluated with serum collected in French Guiana where H. capsulatum is highly endemic. Sera from AIDS patients with disseminated histoplasmosis occurring from 2002 to 2009 and from control HIV-positive patients without histoplasmosis were tested with the GM detection and Histoplasma-specific antibody detection (IEP). In 39 AIDS patients with proven disseminated histoplasmosis, the sensitivity of the Histoplasma IEP was only 35.9% and was linked to the TCD4+ lymphocyte level. For the GM detection, the sensitivity (Se) was 76.9% and specificity (Sp) was 100% with the recommended threshold for aspergillosis diagnosis (0.5). The test was more efficient with a threshold of 0.4 (Se: 0.82 [95% CI: 0.66-0.92], Sp: 1.00 [95% CI: 0.86-1.00], LR+: >10, LR-: 0.18). This study confirms that the GM detection can be a surrogate marker for the diagnosis of disseminated histoplasmosis in AIDS patients in endemic areas where Histoplasma EIA is not available.
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- 2014
7. Paediatric Bone and Joint Infections in French Guiana: A 6 Year Retrospective Review.
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Osei, Lindsay, El Houmami, Nawal, Minodier, Philippe, Sika, Anicet, Basset, Thierry, Seligmann, Hervé, Terraz, Anne, Demar, Magalie, Pochard, Jacques, Clouzeau, Jérôme, Fournier, Pierre-Edouard, Elenga, Narcisse, and by
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PEDIATRIC epidemiology ,BONE diseases in children ,JOINT infections ,CHILDREN ,METHICILLIN-resistant staphylococcus aureus ,OSTEOARTHRITIS ,ANTIBIOTICS ,OSTEOMYELITIS diagnosis ,BACTERIAL toxins ,INFECTIOUS arthritis ,OSTEOMYELITIS ,STAPHYLOCOCCAL diseases ,STAPHYLOCOCCUS aureus ,TOXINS ,RETROSPECTIVE studies ,CYTOTOXINS ,DIAGNOSIS - Abstract
The epidemiology of paediatric bone and joint infections from South America is poorly known. We herein report a retrospective study conducted in whole French Guiana from January 2010 to December 2015. Medical charts of 55 previously healthy children were analysed, identifying 27 with osteomyelitis, 22 with septic arthritis and 6 with multifocal infections and/or osteoarthritis. The male:female ratio was 2.2:1, and the mean age was 7.5 years. Eighty percent children were ≥36 months old who had predominantly osteomyelitis related to methicillin-susceptible Staphylococcus aureus (p < 0.05) in the course of neglected skin infections. Five children presented with multi-systemic infections resulting in one fatality, mainly caused by S. aureus producing Panton-Valentine leucocidin (p < 0.01). In contrast, children aged 6-36 months had more likely culture-negative infections (p < 0.05), septic arthritis and mild clinical and biological features. Further prospective studies are required to better guide rational diagnostic and therapeutic strategies. [ABSTRACT FROM AUTHOR]
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- 2017
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8. A predictive score for hypotension in patients with confirmed dengue fever in Cayenne Hospital, French Guiana.
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Djossou, Félix, Vesin, Guillaume, Elenga, Narcisse, Demar, Magalie, Epelboin, Loïc, Walter, Gaëlle, Abboud, Philippe, Le-Guen, Thierry, Rousset, Dominique, Moreau, Brigitte, Mahamat, Aba, Malvy, Denis, and Nacher, Mathieu
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DENGUE ,HYPOTENSION ,RISK factors of epidemics ,HEALTH services administration ,DIAGNOSIS ,PATIENTS - Abstract
Background: Identifying patients at risk of developing severe dengue is challenging. The objective of the present study was to determine the incidence of hypotension and its predictive factors during the Dengue 2 epidemic in 2013. Methods: In 2013, a longitudinal study was performed using data from all confirmed cases of dengue seen in Cayenne General Hospital. The analysis used Cox proportional modeling to obtain adjusted hazards ratios for hypotension. Results: A total of 806 confirmed patients were included 78 (9.6%) of whom developed hypotension. Extensive purpura, cutaneomucous hemorrhage, serous effusion and age 1–15 years were associated with subsequent hypotension whereas ‘aches’ and a rash were associated with a lower incidence of hypotension. The biological variables independently associated with hypotension were: increase of hematocrit, low protein concentrations, low sodium concentration and lymphocytes over 1400/ml. A risk score was computed from the scaled Cox model coefficient. Conclusions: From a clinician’s perspective, extensive purpura, cutaneomucous hemorrhage, serous effusion, age 1–15 years, hematocrit increase, low protein, low sodium, lymphocytosis and the absence of aches or of a rash, may be important warning signs to predict subsequent hypotension and shock. Over half of the patients with the highest risk score subsequently developed hypotension. The prognostic score had a 48.2% sensitivity with less than 10% of false positives. This score requires external validation before its impact on clinical practice is evaluated. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Q Fever in French Guiana: Tip of the Iceberg or Epidemiological Exception?
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Epelboin, Loïc, Nacher, Mathieu, Mahamat, Aba, Pommier de Santi, Vincent, Berlioz-Arthaud, Alain, Eldin, Carole, Abboud, Philippe, Briolant, Sébastien, Mosnier, Emilie, Mendonça Gomes, Margarete do Socorro, Vreden, Stephen G., Pierre-Demar, Magalie, Lacerda, Marcus, Raoult, Didier, Sampaio de Lemos, Elba Regina, and Djossou, Félix
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Q fever ,COXIELLA burnetii ,ZOONOSES ,HEALTH of Latin Americans ,INFECTIOUS disease transmission ,SURVEILLANCE in literature ,PUBLIC health surveillance ,DIAGNOSIS ,ECOLOGY - Abstract
The author discusses the Q fever, a cosmopolitan zoonosis caused by Coxiella burnetii, an intracellular bacterium. It provides a review of the literature presenting a scarce result of Q fever in Latin America. It mentions high incidence and prevalence of pneumonias in French Guiana with near absence of data in neighbouring countries, suggesting a rapid diagnostic test, techniques and better surveillance systems to map Q fever in Latin America.
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- 2016
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10. Frequency and distribution of mixed Plasmodium falciparum-vivax infections in French Guiana between 2000 and 2008.
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Ginouves, Marine, Veron, Vincent, Musset, Lise, Legrand, Eric, Stefani, Aurélia, Prevot, Ghislaine, Demar, Magalie, Djossou, Félix, Brousse, Paul, Nacher, Mathieu, and Carme, Bernard
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PLASMODIIDAE ,PLASMODIUM ,DIAGNOSIS ,INFECTION ,SENSITIVITY analysis ,POLYMERASE chain reaction - Abstract
Background: The two main plasmodial species in French Guiana are Plasmodium vivax and Plasmodium falciparum whose respective prevalence influences the frequency of mixed plasmodial infections. The accuracy of their diagnosis is influenced by the sensitivity of the method used, whereas neither microscopy nor rapid diagnostic tests allow a satisfactory evaluation of mixed plasmodial infections. Methods: In the present study, the frequency of mixed infections in different part of French Guiana was determined using real time PCR, a sensitive and specific technique. Results: From 400 cases of malaria initially diagnosed by microscopy, real time PCR showed that 10.75 % of the cases were mixed infections. Their prevalence varied considerably between geographical areas. The presence, in equivalent proportions, of the two plasmodial species in eastern French Guiana was associated with a much higher prevalence of mixed plasmodial infections than in western French Guiana, where the majority of the population was Duffy negative and thus resistant to vivax malaria. Conclusion: Clinicians must be more vigilant regarding mixed infections in co-endemic P. falciparum/P. vivax areas, in order to deliver optimal care for patients suffering from malaria. This may involve the use of rapid diagnostic tests capable of detecting mixed infections or low density single infections. This is important as French Guiana moves towards malaria elimination. [ABSTRACT FROM AUTHOR]
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- 2015
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11. Acute Lung, Heart, Liver, and Pancreatic Involvements with Hyponatremia and Retinochoroiditis in a 33-Year-Old French Guianan Patient.
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Groh, Matthieu, Faussart, Alexandra, Villena, Isabelle, Ajzenberg, Daniel, Carme, Bernard, Demar, Magalie, Joly, Véronique, Houze, Sandrine, Simon, Stéphane, Aubert, Dominique, Charlois-Ou, Cécile, and Yeni, Patrick
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FRENCH Guianese ,HYPONATREMIA ,PANCREATITIS ,MACROLIDE antibiotics ,HOSPITALS ,TOXOPLASMA gondii ,DIAGNOSIS - Abstract
The article presents a case study of a 33-year-old French Guianan admitted in the Bichat hospital in Paris, France with a 3-week history of fever associated with confusion and dyspnea. Blood examination reveals hyponatremia, pancreatitis, mild hepatitis, and elevated cardic markers. The patient administered macrolide therapy and gold standard anti-toxoplasmosis treatment was also started after the serological diagnosis of Toxoplasma gondii (T. gondii) infection.
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- 2012
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12. Usefulness of C-Reactive Protein in Differentiating Acute Leptospirosis and Dengue Fever in French Guiana.
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Turnier, Paul Le, Bonifay, Timothée, Mosnier, Emilie, Schaub, Roxane, Jolivet, Anne, Demar, Magalie, Bourhy, Pascale, Nacher, Mathieu, Djossou, Félix, and Epelboin, Loïc
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DENGUE ,LEPTOSPIROSIS ,C-reactive protein ,CREATINE kinase ,BIVARIATE analysis ,DENGUE hemorrhagic fever - Abstract
Objective Leptospirosis and dengue fever (DF) are hard-to-differentiate diseases in cocirculating areas, especially during DF epidemics. Misdiagnosis and ensuing lack of antibiotic therapy can be detrimental in leptospirosis. The objective of this study was to identify factors that help differentiate acute leptospirosis from dengue fever on admission. Method Patients with leptospirosis (positive serology or polymerase chain reaction) were compared with patients with DF (positive nonstructural 1 [NS1] antigen) in a case-control study with age matching. Data on admission were compared using bivariate analysis and multivariate analysis. Results Seventy-two patients with leptospirosis were compared to 216 patients with DF. In bivariate analysis, the factors associated with leptospirosis were male gender, cough, anemia, and elevated blood levels of C-reactive protein (CRP), leukocytes, creatinine, bilirubin, and creatine phosphokinase. Exanthema, purpura, myalgia, headache, and neutropenia were associated with DF. In multivariate analysis, elevated blood levels of leukocytes, bilirubin, and CRP were associated with leptospirosis. The CRP threshold of 50 mg/L taken alone had elevated sensitivity and specificity. Conclusions The CRP level, an easy-to-obtain biomarker, was a powerful tool to differentiate on admission leptospirosis and DF. Facing a dengue-like syndrome in cocirculating areas and awaiting new specific rapid diagnostic tests, CRP dosing could help the clinician to promptly consider the diagnosis of leptospirosis and initiate antibiotic therapy early. [ABSTRACT FROM AUTHOR]
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- 2019
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13. Evolution of the prevalence of intestinal parasitic infections among the French armed forces in French Guiana.
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Christen, Jacques-Robert, Mura, Marie, Roudaut, Gwenaëlle, Drogoul, Anne-Sophie, Demar, Magalie, Briolant, Sébastien, Garnotel, Eric, Simon, Fabrice, and Pommier De Santi, Vincent
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EOSINOPHILIA ,GOLD ,HELMINTHIASIS ,INTESTINAL parasites ,MINERAL industries ,MILITARY personnel ,DISEASE prevalence ,CROSS-sectional method ,DIAGNOSIS - Abstract
Two cross-sectional studies were performed 2 years apart in French military personnel deployed from France to French Guiana. In 2011, military medical centres in French Guiana reported 40 cases of intestinal parasitism in service members returning from illegal gold mining sites in the rainforest. In 2013, 48 out of 132 service members returning from French Guiana after a 4-month mission had eosinophilia and seven were infected with hookworm. A presumptive first-line treatment with albendazole could be the most pragmatic strategy. [ABSTRACT FROM AUTHOR]
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- 2016
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