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BACKGROUND: Circular RNAs (circRNAs) were initially regarded as by-products of aberrant splicing. Present research on their mechanism has gradually penetrated to the molecular level. Many studies have shown that circRNAs participate in osteogenic differentiation through various ways and play a key role. OBJECTIVE: To discuss the research progress of circRNAs during osteogenic differentiation in human mesenchymal stem cells. METHODS: Using “circRNAs, human mesenchymal stem cells, osteogenic differentiation” as keywords in English and Chinese. PubMed, Web of Science, CNKI, and Wanfang were searched for relevant articles on circRNAs in human mesenchymal stem cells published from 1993 to 2020. Types of retrieved articles included reviews, research papers, and books. RESULTS AND CONCLUSION: CircRNAs play an important role in the osteogenesis of mesenchymal stem cells. These circRNAs have proven to be key targets for the diagnosis and treatment of bone diseases. However, the molecular mechanism of circRNA in osteogenic differentiation needs further research, and in vivo experiments are also needed to support existing conclusions. [ABSTRACT FROM AUTHOR]

Objective To clarify the role of discs large homolog 5 (DLG5) in the diagnosis and prognosis of renal clear cell carcinoma (ccRCC) by studying the expression of DLG5 in the clinical tissue of ccRCC. Methods DLG5 expression in ccRCC tissues and normal renal tissue was measured by using the Cancer Genome Atlas (TCGA) Database and Gene Expression Omnibus (GEO) Database. The correlation of DLG5 expression with the prognosis and clinicopathological parameters of ccRCC was analyzed by LinkedOmics and GEPIA. The interactions protein network of DLG5 was predicted by String-DB. Results Compared with that of the normal kidney tissues, DLG5 expression was significantly upregulated in the ccRCC tissues (P <0.05). The high DLG5 expression was an independent prognostic factor affecting the overall survival rate of ccRCC based on TCGA (P =2.997×10-5). Furthermore, DLG5 expression had a positive correlation with the TNM stage, ethnicity, tumor purity, year-tobirth, PCNA (P =1.1×10-6, R =0.19), and RAD1 (P =5.7×10-6, R =0.18) expression of ccRCC. Conclusion In ccRCC, the high expression of DLG5 is a significant index of poor prognosis. Therefore, DLG5 can be a potential biomarker for predicting the metastasis, recurrence and prognosis of the patients. [ABSTRACT FROM AUTHOR]