1. Peanut skin procyanidins reduce intestinal glucose transport protein expression, regulate serum metabolites and ameliorate hyperglycemia in diabetic mice.
- Author
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Liu, Min, Shen, Jinxin, Zhu, Xiaoling, Ju, Tingting, Willing, Benjamin P., Wu, Xin, Lu, Qun, and Liu, Rui
- Subjects
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PROCYANIDINS , *PROTEIN expression , *METABOLITES , *GLUCOSE transporters , *HYPERGLYCEMIA , *INSULIN sensitivity , *BLOOD sugar , *INSULIN , *BILE acids - Abstract
[Display omitted] • PSP inhibit sucrase, maltase and Na+/K+-ATPase activity in the jejunum. • PSP reduce gut glucose transport capacity with reduced expression of SGLT1and GLUT2. • PSP inhibit SGLT1 and GLUT2 protein expressions related to PLCβ2/PKC signalling pathways. • PSP mainly affect fatty acid, amino acid and bile acid metabolic pathways in the serum of diabetic mice. One of diabetic characteristics is the postprandial hyperglycemia. Inhibiting glucose uptake may be beneficial for controlling postprandial blood glucose levels and regulating the glucose metabolism Peanut skin procyanidins (PSP) have shown a potential for lowering blood glucose; however, the underlying mechanism through which PSP regulate glucose metabolism remains unknown. In the current study, we investigated the effect of PSP on intestinal glucose transporters and serum metabolites using a mouse model of diabetic mice. Results showed that PSP improved glucose tolerance and systemic insulin sensitivity, which coincided with decreased expression of sodium-glucose cotransporter 1 and glucose transporter 2 in the intestinal epithelium induced by an activation of the phospholipase C β2/protein kinase C signaling pathway. Moreover, untargeted metabolomic analysis of serum samples revealed that PSP altered arachidonic acid, sphingolipid, glycerophospholipid, bile acids, and arginine metabolic pathways. The study provides new insight into the anti-diabetic mechanism of PSP and a basis for further research. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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