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Your search keyword '"Auster, Michael E."' showing total 2 results
Start Over Author "Auster, Michael E." Topic diabetic foot
2 results on '"Auster, Michael E."'

1. Alginate and DNA Gels Are Suitable Delivery Systems for Diabetic Wound Healing.

Author

Tellechea A, Silva EA, Min J, Leal EC, Auster ME, Pradhan-Nabzdyk L, Shih W, Mooney DJ, and Veves A

Subjects
Animals, Bandages, Biocompatible Materials administration & dosage, Drug Carriers, Gels, Glucuronic Acid administration & dosage, Hexuronic Acids administration & dosage, Mice, Mice, Inbred C57BL, Alginates administration & dosage, Diabetes Mellitus, Experimental drug therapy, Diabetic Foot drug therapy, Drug Delivery Systems, Vascular Endothelial Growth Factor A administration & dosage, Wound Healing drug effects
Abstract

Diabetic foot ulcers (DFU) represent a severe health problem and an unmet clinical challenge. In this study, we tested the efficacy of novel biomaterials in improving wound healing in mouse models of diabetes mellitus (DM). The biomaterials are composed of alginate- and deoxyribonucleic acid (DNA)-based gels that allow incorporation of effector cells, such as outgrowth endothelial cells (OEC), and provide sustained release of bioactive factors, such as neuropeptides and growth factors, which have been previously validated in experimental models of DM wound healing or hind limb ischemia. We tested these biomaterials in mice and demonstrate that they are biocompatible and can be injected into the wound margins without major adverse effects. In addition, we show that the combination of OEC and the neuropeptide Substance P has a better healing outcome than the delivery of OEC alone, while subtherapeutic doses of vascular endothelial growth factor (VEGF) are required for the transplanted cells to exert their beneficial effects in wound healing. In summary, alginate and DNA scaffolds could serve as potential delivery systems for the next-generation DFU therapies., (© The Author(s) 2015.)

Published
2015
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2. Increased skin inflammation and blood vessel density in human and experimental diabetes

Author

Eugenia Carvalho, Francesco Tecilazich, Michael E. Auster, Sarada Kuchibhotla, Leena Pradhan Nabzdyk, Aristidis Veves, Iraklis Kontoes, Antonios Kafanas, Jacqueline Paolino, Ana Tellechea, Ermelindo C. Leal, Tellechea, Ana, Kafanas, Antonio, Leal, Ermelindo C., Tecilazich, Francesco, Kuchibhotla, Sarada, Auster, Michael E., Kontoes, Irakli, Paolino, Jacqueline, Carvalho, Eugenia, Nabzdyk, Leena Pradhan, and Veves, Aristidis

Subjects
Male, Pathology, Biopsy, diabetes, skin blood vessels, skin changes, skin inflammation, wound healing, Animals, Blood Vessels, Dermatitis, Diabetes Mellitus, Experimental, Diabetic Foot, Disease Progression, Female, Follow-Up Studies, Humans, Mice, Mice, Inbred BALB C, Middle Aged, Prospective Studies, Rabbits, Rats, Rats, Sprague-Dawley, Regional Blood Flow, Skin, Surgery, Rabbit, Systemic inflammation, skin change, Inbred BALB C, medicine.diagnostic_test, integumentary system, General Medicine, Diabetic foot ulcer, medicine.anatomical_structure, medicine.symptom, Blood vessel, Human, medicine.medical_specialty, Blood Vessel, Inflammation, Dermatiti, Article, Follow-Up Studie, Experimental, Diabetes mellitus, medicine, Diabetes Mellitus, business.industry, Animal, medicine.disease, Diabetic foot, Prospective Studie, diabete, Immunology, skin blood vessel, Rat, Sprague-Dawley, Wound healing, business
Abstract

Systemic inflammation is associated with impaired wound healing in diabetes mellitus (DM) patients. Using immunohistochemistry techniques, the authors investigated changes in skin inflammation and skin blood vessels in human and experimental diabetes. Comparing to the non-DM human subjects, the total number of inflammatory cells per biopsy and the number of inflammatory cells around blood vessels, a strong indication of inflammation, were higher in DM subjects irrespective of their risk for developing diabetic foot ulcer. Inflammatory cell infiltration was robustly increased in all DM animal models compared with their non-DM controls. The number and density of blood vessels and CD31 positive proliferating endothelial cells around preexisting skin vessels was also higher in the DM patients. However, there were no differences in the skin blood flow between the non-DM and DM subjects. The number of skin blood vessels was also increased in the DM animals; however, these differences were less obvious than the ones observed for inflammatory cells. We conclude that skin inflammation and skin blood vessel density is increased in diabetic human subjects and in rodent and rabbit models of diabetes.

Published
2013

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