Search

Background: We investigated whether vitamin C has protective effects on rat liver tissue treated with different dexmedetomidine doses., Material and Methods: Thirty five wistar albino rats were randomly divided into 5 groups (Control (0.9% NaCl intraperitoneally (i.p.), Dexmedetomidine 5 μg.kg(-1) (i.p.), Dexmedetomidine 5 μg.kg(-1) i.p. plus Vitamin C (100 mg.kg(-1)), Dexmedetomidine 10 μg.kg(-1) i.p. and Dexmedetomidine 10 μg.kg(-1) i.p. plus Vitamin C (100 mg.kg(-1)). Histopathological liver injury, superoxide dismutase (SOD) activity and tissue Malondialdehyde levels were investigated., Results: Hepatocyte degeneration was significantly higher in D10 group than those in other study groups (p < 0.0001, p = 0.002, p < 0.0001, p = 0.005, respectively). Similarly, liver tissue sinusoidal dilatation and hepatocyte necrosis were significantly higher in D10 group than those in other groups (p < 0.0001, p < 0.0001, p = 0.002, p < 0.0001 and p < 0.0001, p = 0.046, p < 0.0001 and p = 0.002, respectively). Tissue MDA levels in D10 group were significantly higher than those in control, D5+Vit C and D10+Vit C groups (p = 0.028, p = 0.004, p = 0.031, respectively). SOD enzyme activity in D10 group was significantly lower than in control, D5+Vit C and D10+Vit C groups (p < 0.0001, p = 0.023 and p = 0.031, respectively)., Conclusion: High dose dexmedetomidine can induce hepatic injury and oxidative stress in rats while pre-treatment with vitamin C may be effective in protecting liver tissue against this newly recognized undesirable dexmedetomidine effect (Tab. 2, Fig. 5, Ref. 30).

Hasan Bostanc&inodot;,1 Selin Erel,2 Ay&scedil;egül Küçük,3 Gülay Kip,2 &Scedil;aban Cem Sezen,4 Seda Gokgoz,5 Muharrem Atl&inodot;,4 Feyza Aktepe,2 Kursat Dikmen,1 Mustafa Arslan,2,6,7 Mustafa Kavutçu5 1Gazi University Faculty of Medicine, Department of General Surgery, Ankara, Turkey; 2Gazi University Faculty of Medicine Department of Anesthesiology and Reanimation, Ankara, Turkey; 3Kutahya Health Sciences University Faculty of Medicine, Department of Physiology, Kutahya, Turkey; 4K&inodot;r&inodot;kkale University Faculty of Medicine, Department of Histology and Embryology, K&inodot;r&inodot;kkale, Turkey; 5Gazi University Faculty of Medicine, Department of Medical Biochemistry, Ankara, Turkey; 6Gazi University, Life Sciences Application and Research Center, Ankara, Turkey; 7Gazi University, Laboratory Animal Breeding and Experimental Research Center (GUDAM), Ankara, TurkeyCorrespondence: Ay&scedil;egül Küçük, Kutahya Health Sciences University, Medical Faculty, Department of Physiology, Kutahya, Turkey, Tel +90 532 675 77 05, Email aysegul.kucuk@gmail.comObjective: Pancreatic surgeries inherently cause ischemia-reperfusion (IR) injury, affecting not only the pancreas but also distant organs. This study was conducted to explore the potential use of dexmedetomidine, a sedative with antiapoptotic, anti-inflammatory, and antioxidant properties, in mitigating the impacts of pancreatic IR on kidney and liver tissues.Methods: A total of 24 rats were randomly divided into four groups: control (C), dexmedetomidine (D), ischemia reperfusion (IR), and dexmedetomidine ischemia reperfusion (D-IR). Pancreatic ischemia was induced in the IR and D-IR groups. Dexmedetomidine was administered intraperitoneally to the D and D-IR groups. Liver and kidney tissue samples were subjected to microscopic examinations after hematoxylin and eosin staining. The levels of thiobarbituric acid reactive substances (TBARS), aryllesterase (AES), catalase (CAT), and glutathione S-transferase (GST) enzyme activity were assessed in liver and kidney tissues. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine were measured.Results: A comparison of the groups revealed that the IR group exhibited significantly elevated TBARS (p

Necmiye &Scedil;engel,1 Zeynep Köksal,2 Ali Do&gbreve;an Dursun,3 Ömer Kurtipek,2 &Scedil;aban Cem Sezen,4 Mustafa Arslan,2 Mustafa Kavutçu5 1Department of Oral and Maxillofacial Surgery, Gazi University Faculty of Dentistry, Ankara, Turkey; 2Department of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara, Turkey; 3Department of Physiology, At&inodot;l&inodot;m University Faculty of Medicine, Ankara, Turkey; 4Department of Histology and Embryology, K&inodot;r&inodot;kkale University Faculty of Medicine, K&inodot;r&inodot;kkale, Turkey; 5Department of Medical Biochemistry, Gazi University Faculty of Medicine, Ankara, TurkeyCorrespondence: Mustafa Arslan, Gazi University, Medical Faculty, Department of Anesthesiology and Reanimation, Ankara, 06510, Turkey, Tel +90 533 422 85 77, Email mustarslan@gmail.comBackground: Ischaemia–reperfusion (IR) injury, which can be encountered during surgical procedures involving the abdominal aorta, is a complex process that affects distant organs, such as the heart, liver, kidney, and lungs, as well as the lower extremities. In this study, we aimed to contribute to the limited literature by investigating the protective effect of dexmedetomidine, which was administered through different routes, on kidney tissue in rats with spinal cord IR injury.Methods: A total of 30 rats were randomly divided into five groups: control (C group), IR (IR group), IR-intraperitoneal dexmedetomidine (IRIPD group), IR-intrathecal dexmedetomidine (IRITD group), and IR-intravenous dexmedetomidine (IRIVD group). The spinal cord IR model was established. Dexmedetomidine was administered at doses of 100 μg/kg intraperitoneally, 3 μg/kg intrathecally, and 9 μg/kg intravenously. Histopathologic parameters in kidney tissue samples taken at the end of the reperfusion period and biochemical parameters in serum were evaluated.Results: When examined histopathologically, tubular dilatation was found to be significantly reduced in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.012, all). Vascular vacuolization and hypertrophy were significantly decreased in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.006, all). Tubular cell degeneration and necrosis were significantly reduced in the IRIVD, IRITD, and IRIPD groups compared with the IR group (p = 0.008, p = 0.08, and p = 0.030, respectively). Lymphocyte infiltration was significantly decreased in the IRIVD and IRITD groups compared with the IR group (p = 0.006 and p = 0.06, respectively).Conclusion: It was observed that dexmedetomidine administered by different routes improved the damage caused by IR in kidney histopathology. We think that the renoprotective effects of dexmedetomidine administered intravenously and intrathecally before IR in rats are greater.Keywords: dexmedetomidine, ischaemia–reperfusion, intrathecal, intravenous, rat