Search

In this work on Rhodotorula gracilis, we studied the level of the representative enzymes of foundamental metabolic ways (HK, PK, G6PDH and IDH) and those of ICL and MS key enzymes of glyoxylate cycle with different carbone sources. The glucose appears the best source of carbone for all the tested enzymes except ICL and MS which are practically absent in the cells developed with this carbone source. In presence of acetate and ethanol we have obtained the expression of the glyoxylate cycle enzymes (ICL and MS). Moreover we obtained the operativity of the glyoxylate cycle in the lipid-rich cells or cells developped on neutral detergents or anionics similar to the fatty acids.

We describe here the effects of natural and synthetic detergents on the D-glucose transport into brush-border membranes of vesicles of rabbit's intestine. Two synthetic detergents: Triton X-100 and dodecyltrimethylammonium bromide have been found very strong inhibitors (more than 50 p. 100 of inhibition of maximal D-glucose uptake). Kinetic studies showed that these detergents behaved as mixed type inhibitors. The Na+-dependent transport of amino acids (aspartic acid, lysine, phenylalanine) is only poorly affected by dodecyltrimethylammonium bromide, while Triton X-100 inhibits unspecifically all the transport studied.