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Simple Summary: More and more studies are suggesting the role of microbes in several diseases in addition to the germline and environmental factors. F. nucleatum is recently being associated with colorectal cancer and here, we aimed to identify important drug targets from the core genome of colorectal cancer associated F. nucleatum through bioinformatics approach. We used one drug target for further analysis and obtained natural product inhibitors against it. Finally, we validated inhibition stability through dynamics simulation approach. We are hopeful that this study could benefit researchers working on colorectal cancer, its microbiome and cure. Colorectal cancer (CRC) ranks third among all cancers in terms of prevalence. There is growing evidence that gut microbiota has a role in the development of colorectal cancer. Fusobacterium nucleatum is overrepresented in the gastrointestinal tract and tumor microenvironment of patients with CRC. This suggests the role of F. nucleatum as a potential risk factor in the development of CRC. Hence, we aimed to explore whole genomes of F. nucleatum strains related to CRC to predict potential therapeutic markers through a pan-genome integrated subtractive genomics approach. In the current study, we identified 538 proteins as essential for F. nucleatum survival, 209 non-homologous to a human host, and 12 as drug targets. Eventually, riboflavin synthase (RiS) was selected as a therapeutic target for further processing. Three different inhibitor libraries of lead-like natural products, i.e., cyanobactins (n = 237), streptomycins (n = 607), and marine bacterial secondary metabolites (n = 1226) were screened against it. After the structure-based study, three compounds, i.e., CMNPD3609 (−7.63) > Malyngamide V (−7.03) > ZINC06804365 (−7.01) were prioritized as potential inhibitors of F. nucleatum. Additionally, the stability and flexibility of these compounds bound to RiS were determined via a molecular dynamics simulation of 50 ns. Results revealed the stability of these compounds within the binding pocket, after 5 ns. ADMET profiling showed compounds as drug-like, non-permeable to the blood brain barrier, non-toxic, and HIA permeable. Pan-genomics mediated drug target identification and the virtual screening of inhibitors is the preliminary step towards inhibition of this pathogenic oncobacterium and we suggest mouse model experiments to validate our findings. [ABSTRACT FROM AUTHOR]

Aims:To explore physical activity (PA) habits among the medical students and examine the correlation with their grade point average (GPA) achievement at College of Medicine, King Saud University, Riyadh, Saudi Arabia. Methods:A cross-sectional study was conducted among the medical students (n = 409), during the academic year 2012–2013. Students’ physical activity habits were self-reported. GPA, body mass index (BMI) and body fat percent (BF%) were collected and analyzed with SPSS software. Results:Out of 409 students, 193 (47.2%) students reported being physically active. Our result showed a significant positive association between students’ PA habits and high-GPA achievement (χ2 = 10.65,p = 0.001). The greatest odds ratio of high GPA was found among the fourth year students (OR = 3.08, CI: 1.15–8.28,p = 0.025) and fifth year students (OR = 5.07, CI: 1.48–17.31,p = 0.010). In addition, significant association was found between the normal BMI and high-GPA achievers (χ2 = 8.30;p = 0.016). However, no statistically, significant association was found between BF% and GPA. Conclusions:The present study showed a positive associations was found between PA habits and high academic achievement. In addition, positive association was found between PA, obese students and GPA achievement. Therefore, there is a need for the establishment of physical activity education and public health programs to promote importance of PA in Saudi population. [ABSTRACT FROM PUBLISHER]