7 results on '"Danfeng Qian"'
Search Results
2. <scp>TT</scp> genotype of rs10036748 in <scp>TNIP</scp> 1 shows better response to methotrexate in a Chinese population: a prospective cohort study
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Qiaohu Xu, Zhi-Zhong Zheng, Zhenghua Zhang, Mingshun Wu, Xuejun Zhang, Yuling Chang, Ling Jin, Ling Han, Danfeng Qian, Xu Fang, Qun Zhang, Nikhil Yawalkar, Qiong Huang, Kexiang Yan, Xueying Li, Y. Zhang, and Xu Jinhua
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Adult ,Male ,China ,medicine.medical_specialty ,Genotyping Techniques ,Drug Resistance ,Arthritis ,Dermatology ,Polymorphism, Single Nucleotide ,Severity of Illness Index ,Gastroenterology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Psoriasis Area and Severity Index ,Psoriasis ,Internal medicine ,Genotype ,medicine ,Humans ,Genetic Predisposition to Disease ,Prospective Studies ,Prospective cohort study ,Aged ,business.industry ,Confounding ,Middle Aged ,medicine.disease ,DNA-Binding Proteins ,Methotrexate ,Treatment Outcome ,Concomitant ,Female ,Dermatologic Agents ,business ,medicine.drug - Abstract
BACKGROUND Methotrexate (MTX) is an efficacious treatment for psoriasis; however, its widespread application is limited by its unpredictable efficacy. OBJECTIVES To investigate the association of clinical factors and variants of psoriasis susceptibility genes with clinical responses to MTX in a prospective cohort. METHODS A total of 221 patients with psoriasis were recruited. Patients who achieved Psoriasis Area and Severity Index (PASI) improvement ≥ 75% at week 12 were defined as responders, whereas those with PASI improvement < 50% were defined as nonresponders. In 90 screening patients, genetic variants for 18 single-nucleotide polymorphisms in 14 susceptibility genes, and HLA-Cw6 status were initially compared for responders and nonresponders. Statistically significant associations in genetic variants were verified in all 221 patients. RESULTS Overall, 49% and 45% of patients achieved PASI 75 improvement during screening and verification stages, respectively. Concomitant arthritis with psoriasis and high body mass index (BMI) negatively affect the efficacy of MTX. TT genotype of rs10036748 in TNIP1 was significantly associated with PASI 75 response at week 12 (54% and 37%, P < 0·05). A significantly higher PASI 90 response was observed in patients with TT genotype of rs10036748 (27% vs. 12%, P < 0·01) and TC/TT genotype of rs4112788 in LCE3D (25% vs. 13%, P < 0·05) at week 12 compared with those who had other genotypes. After adjustment for all confounding factors, only BMI (P < 0·05), arthritis (P < 0·05) and genotype of rs10036748 (P < 0·05) were significantly associated with clinical responses to MTX. CONCLUSIONS Patients with psoriasis with TT genotype of rs10036748 in TNIP1, with lower BMI, without arthritis will achieve a better response to MTX.
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- 2019
3. Exome-Wide Rare Loss-of-Function Variant Enrichment Study of 21,347 Han Chinese Individuals Identifies Four Susceptibility Genes for Psoriasis
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Chao Yang, Mengyun Chen, He Huang, Xueying Li, Danfeng Qian, Xiaojie Hong, Lijun Zheng, Jiaqi Hong, Zhengwei Zhu, Xiaodong Zheng, Yujun Sheng, and Xuejun Zhang
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0301 basic medicine ,Genetic Markers ,Male ,China ,Genotype ,Genome-wide association study ,Susceptibility gene ,Dermatology ,Biology ,Biochemistry ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Psoriasis ,medicine ,Ethnicity ,Humans ,Exome ,Genetic Predisposition to Disease ,Molecular Biology ,Gene ,Genotyping ,Loss function ,Genetics ,Cell Biology ,medicine.disease ,030104 developmental biology ,030220 oncology & carcinogenesis ,Human genome ,Female ,Genome-Wide Association Study - Abstract
Most psoriasis-related genes or loci identified by GWAS represent common clusters and are located in noncoding regions of the human genome, providing only limited evidence for the roles of rare coding variants in psoriasis. Two exome-wide case-control genotyping data sets (11,245 cases and 11,177 controls) were obtained from our previous study. Quality controls were established for each data set, and the markers remaining in each set were annotated using ANNOVAR. Gene-based analysis was performed on the annotation results. A total of 250 and 35 genes in the Exome_Fine and Exome_Asian array cohorts, respectively, exceeded the threshold (P4.43 × 10
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- 2019
4. Association analysis of the major histocompatibility complex region in psoriasis vulgaris
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S. Yang, X. Zheng, Delin Ran, Yi Zhou, Qiaohu Xu, Yujun Sheng, He Huang, Leilei Wen, Y. Zhang, Qun Zhang, Ling Jin, Wen-Xian Li, X. Zhang, Danfeng Qian, Minglong Cai, Zheng wei Zhu, and Lili Tang
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biology ,Genotyping Techniques ,business.industry ,Genes, MHC Class I ,Dermatology ,Major histocompatibility complex ,medicine.disease ,Polymorphism, Single Nucleotide ,Healthy Volunteers ,Major Histocompatibility Complex ,Psoriasis ,Case-Control Studies ,Immunology ,biology.protein ,Medicine ,Humans ,Genetic Predisposition to Disease ,business ,Medical History Taking ,Alleles ,Genetic association - Published
- 2019
5. Safety and Efficacy of Methotrexate for Chinese Adults With Psoriasis With and Without Psoriatic Arthritis
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Xu Fang, Xuejun Zhang, Qiong Huang, Ling Han, Qiaohu Xu, Kexiang Yan, Xu Jinhua, Y. Zhang, Yuling Chang, Xueying Li, Danfeng Qian, Zhenghua Zhang, Mingshun Wu, Zhi-zhong Zheng, Nikhil Yawalkar, Qun Zhang, and Ling Jin
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medicine.medical_specialty ,business.industry ,Arthritis ,Dermatology ,medicine.disease ,Gastroenterology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Psoriatic arthritis ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Psoriasis ,Internal medicine ,Severity of illness ,medicine ,Outpatient clinic ,Methotrexate ,business ,Prospective cohort study ,Adverse effect ,610 Medicine & health ,medicine.drug ,Original Investigation - Abstract
Importance It is necessary to determine whether psoriasis responds to methotrexate in the same manner in patients with and without psoriatic arthritis. Objective To evaluate the effectiveness and safety of methotrexate in treating patients with psoriasis with and without psoriatic arthritis. Design, Setting, and Participants In this prospective, single-arm, interventional study, a total of 235 patients with psoriasis, 107 without psoriatic arthritis and 128 with psoriatic arthritis who were receiving methotrexate therapy from April 1, 2015, to December 31, 2017, were recruited from the outpatient department of a hospital at a large Chinese university. There were no significant demographic or clinical differences between the subgroups with the exception of diabetes. Interventions A 12-week course of low-dosage oral methotrexate (7.5-15 mg weekly). Main Outcomes and Measures Changes in disease severity, adverse events, blood cell counts, and liver and renal function. Results A total of 235 patients with psoriasis (166 male [66.0%]; mean [SD] age, 49.6 [15.1] years) received methotrexate treatment for 12 weeks. The 90% reduction from baseline Psoriasis Area Severity Index response was significantly lower in patients with psoriatic arthritis than in patients without psoriatic arthritis at week 8 (4 0f 128 [3.1%] vs 12 of 107 [11.2%];P = .02) and week 12 (19 of 128 [14.8%] vs 27 of 107 [25.2%];P = .049). Furthermore, the incidence of adverse events, including dizziness (12 of 128 [9.4%] vs 1 of 107 [0.9%];P = .007), gastrointestinal symptoms (32 of 128 [25.0%] vs 13 of 107 [12.1%];P = .01), and hepatoxicity (34 of 128 [26.6%] vs 16 of 107 [15.0%];P = .04), was significantly higher in patients with psoriatic arthritis than in patients without psoriatic arthritis. Methotrexate-induced elevation of alanine aminotransferase levels was associated with body mass index (mean [SD] body mass index, 26 [4] in patients with [P = .04] vs 26 [4] in those without [P = .005] psoriatic arthritis) and smoking (17 of 34 [50.0%] in patients with [P = .02] vs 9 of 16 [56.3%] in those without [P = .04] psoriatic arthritis). Conclusions and Relevance In this study, methotrexate was well tolerated and effective in treating psoriasis. It was more effective, with fewer adverse effects, in patients with psoriasis who did not have psoriatic arthritis than in patients who presented with both psoriasis and psoriatic arthritis. Therefore, methotrexate can be recommended as first-line treatment for psoriasis without arthritis.
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- 2019
6. Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer
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Sen Yang, Jinping Gao, Xuejun Zhang, Jing Zhang, Qi Zhen, Danfeng Qian, Yaochi Wang, Xiaomeng Wang, Mengting Liu, Huimin Guo, Xueying Li, Jie Ma, Ling Jin, Mingshun Wu, and Shuangjun Xu
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Proteomics ,Skin barrier ,Dermatology ,Computational biology ,Filaggrin Proteins ,Mass spectrometry ,Orbitrap ,01 natural sciences ,Mass Spectrometry ,law.invention ,010309 optics ,Cornified envelope ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Intermediate Filament Proteins ,law ,0103 physical sciences ,Keratin ,Protein purification ,Medicine ,Chinese subjects ,Humans ,Skin ,chemistry.chemical_classification ,integumentary system ,business.industry ,Proteins ,Healthy Volunteers ,chemistry ,Proteome ,Epidermis ,business ,Software - Abstract
Objective The objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label-free nanoflow liquid chromatography-mass spectrometry (nLC-MS). Materials and methods Five consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin-digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data-dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2). Results In this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier. Conclusion This study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease.
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- 2018
7. HLA-C*01:02 and HLA-A*02:07 Confer Risk Specific for Psoriatic Patients in Southern China
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Wenjuan Wu, Danfeng Qian, Jie Ma, Xiaodong Zheng, Zhengwei Zhu, Delin Ran, Minglong Cai, Chuanliang Zhang, Yujun Sheng, Mingshun Wu, Xuejun Zhang, Xiaojie Hong, Leilei Wen, Lu Liu, Jiaqi Hong, and He Huang
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China ,HLA-A Antigens ,Case-control study ,HLA-C Antigens ,Cell Biology ,Dermatology ,Biology ,Biochemistry ,HLA-A ,Minor allele frequency ,HLA-C ,Southern china ,Case-Control Studies ,Immunology ,Prevalence ,Humans ,Psoriasis ,Genetic Predisposition to Disease ,Molecular Biology - Published
- 2019
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