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BACKGROUND AND AIM: Periodontopathogens require iron constituents for their growth and metabolism in subgingival crevice. In this study, C.rectus was detected and quantified by using 16s rDNA based PCR in chronic periodontitis and compared with the levels of serum iron, total iron binding capacity and transferrin in chronic periodontitis and healthy sites. MATERIALS AND METHODS: One hundred twenty subjects divided into chronic periodontitis and healthy controls. Deep subgingival plaque was collected and genomic DNA was extracted from each sample analysed for C.rectus using 16s rRNA based PCR analysis. Blood samples were collected from both groups for estimation of serum iron, serum total iron binding capacity and serum transferrin levels. The quantified bacterial count was compared with blood samples. C. rectus was detected in both groups. RESULTS: There was significant increase in bacterial count in chronic periodontitis (p

Background and Aim: Selenomonas species have been associated with chronic periodontitis and have been implicated in converting periodontal health to disease. Scanty literature is available in Indian population. Hence, the objective of the study was to detect the prevalence of Selenomonas sputigena in healthy and chronic periodontitis by polymerase chain reaction (PCR) in Indian population and to check whether smoking affects the subgingival microflora of this organism in chronic periodontitis. Materials and Methods: A total of 60 subjects with severe chronic periodontitis with or without smoking and periodontal healthy subjects underwent clinical and microbiological assessment. A deep subgingival plaque sample was collected and genomic DNA was extracted from each sample and analysed for detection of Selnomonas sputigena using PCR. The frequency and quantification of bacteria were also estimated. Results: All groups differed statistically significant in the frequency of detection of Selenomonas sputigena. On comparison of patients with chronic periodontitis in smokers and non-smokers, there was no statistically significant difference. When the results were quantified, statistically nonsignificant results were seen among all groups. Plaque index, gingival index, probing pocket depth and clinical attachment level were statistically non-significant in chronic periodontitis with smokers and non-smokers. Conclusion: Prevalence of Selenomonas sputigena showed significant differences with respect to the frequency of detection when comparing the disease group to the healthy population. But no significant difference was seen when the results were quantified. Smoking has no influence on number of Selenomonas sputigena. This study highlights presence as well as quantity of the organism is very important in elucidating its role in causation and progression of the disease.