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Objective: The aim of this invitro study was to evaluate the cytotoxicity of three different self-adhesiveresin cements.Materials andMethod:Standardized test samples (2×5 mm) of adhesive resin cements ICEM (Heraeus Kulzer, Hanau, Germany), Nova Resin (Imicryl, Konya,Turkey) and Clearfil SACement (Kuraray, Okuyama, Japan) were prepared according to themanufacturers’ instructions. Bovine dentalpulp-derived cells (Clonal SV40) were seeded into the wells of a 96 well-plate,and incubated in MEM Alpha (Gibco Invitrogen) supplemented with 20% fetal bovineserum, 5% penicillin/streptomycin, 1% geneticin in a humidified atmosphere of 5% CO2. The test samples were immersed in thegrowth medium for 24 h and the extracts were applied onto the cells. A group ofcells was immersed in the growth medium that was not exposed to the testmaterials, and was assigned as negative control. The cell viability wasdetermined by enzyme activity tests at the end of a 24 h treatment period. Thedata were statistically analyzed using one-way ANOVA.Results: There was no statistically significant difference betweenthe cell viability of the negative control group and ICEM, Clearfil SA and NovaResin Cement groups (p>0.05). There was also no statistically significantdifference among the self-adhesive resin cement groups (p>0.05).Conclusion: In this in vitrostudy, self-adhesive resin cements were found to have no significant cytotoxiceffects on two dimensional dental bovine pulp cells., Amaç:Bu çalışmanın amacı, klinikte kullanılan kendinden bağlanabilen üç farklıadeziv rezin simanın sitotoksisitesinin değerlendirilmesidir.Gereç ve Yöntem: Çalışmamızda üretici firmanın önerileri dikkate alınarak silindirikşekilli (2x5mm) adeziv rezin siman test örnekleri hazırlandı ICEM (HeraeusKulzer, Hanau, Almanya), Nova Resin (Imicryl, Konya, Turkey) ve Clearfil SACement (Kuraray, Okuyama, Japonya). Sığır pulpasından elde edilmiş hücreler(Clonal SV40) 96-kuyucuklu hücre kültürü plakları içerisine yerleştirildi ve %20fetal sığır serumu, %5 penisilin/streptomisin, %1 genetisin içeren MEM Alphaiçerisinde (Gibco Invitrogen) %5 CO2 ortamında, 37°C’de 24 saat enkübe edildi.Test örnekleri hazırlanan besi ortamı içerisinde 24 saat bekletildi veekstraktlar hücreler üzerine uygulandı. İçerisinde test materyali ekstrakteedilmemiş besi ortamına maruz bırakılan hücreler negatif kontrol grubu kabuledildi. Hücre canlılığı 24 saat muamele sonunda enzim aktivitesi iledeğerlendirildi. Veriler one-way ANOVA ile analiz edildi.Bulgular:ICEM, Clearfil SA ve Nova Resin siman gruplarının canlılık yüzdeleri ilenegatif kontrol grubunun hücre canlılık oranları arasında istatistiksel olarakanlamlı fark bulunmadı (p>0.05). Aynı zamanda rezin siman grupları birbiriile karşılaştırıldığında istatiksel olarak gruplar arasında yine anlamlı farkbulunmadı (p>0.05).Sonuç: Bu in vitro çalışmada iki-boyutlu sığır dentalpulpa hücreleri üzerinde test edilen kendinden bağlanabilen adeziv rezinsimanların önemli derecede sitotoksik etki göstermedikleri tespit edildi.

Purpose To evaluate the effect of a home bleaching agent (Opalescence PF) on the translucency of CAD/CAM ceramic systems. Materials and methods The 28 sintered ceramic specimens (IPS Empress CAD and IPS e.max CAD; 15 mm long, 10 mm wide, 1 mm thick) were divided into two subgroups as control and bleaching groups (n = 7). Carbamide peroxide (CP), 16%, home bleaching agent was applied onto the surface of each specimen for 6 hours per day for 7 days. A spectrophotometer (VITA Easyshade Advance) was used to measure the CIE L* a* b* coordinates and the reflectance value (Y) of the specimens on white and black backgrounds. The translucency parameter (TP), contrast ratio (CR), and opalescence parameter (OP) of the specimens were calculated. The data were statistically analyzed using Kolmogorov-Smirnov, one-way ANOVA, Tukey's test, and Pearson's correlation. Results Statistically significant differences in the TP values after 16% CP bleaching treatment were observed (p ˂ 0.05); however, no significant differences were found in the OP and CR values after the surface treatment (p ˃ 0.05). Conclusions According to our study, patients who have all-ceramic restorations in their mouths should be careful when using home bleaching agents, because whitening agents can affect the translucency of all-ceramic restorations such as e.max CAD and Empress CAD.

OZET Amac: Bu calismanin amaci, farkli apikal caplara sahip (1mm ve 1.4mm) immatur dislere ortograd ve retrograd yollarla, farkli uygulama teknikleri ile yerlestirdigimiz MTA’nin apikal sizintilarinin bilgisayarli sivi filtrasyon teknigi ile belirlenmesidir. Gerec ve yontem: Calismada kullanmak icin toplamda 100 adet cekilmis ust cene keser insan disi kullanildi. Acik apekse sahip immatur disleri simule etmek amaciyla apikal foramen, capi 1mm ve 1.4 mm genisliginde olacak sekilde prepare edildi. Uretici firmanin talimatlari dogrultusunda karistirilan MTA dort farkli yolla (retrograd yolla, ortograd yolla, 2 asamali ortograd yolla ve internal matrix destekli ortograd yolla) farkli apikal foramen capa sahip kok kanallarinin icerisine yerlestirildi. Disler bilgisayarli sivi-filtrasyon cihazinda mikrosizinti testi icin hazirlandi. Sizinti calismasindaki her bir ornekteki sivi hareketi olcumleri 15 saniye araliklarla 8 kez tekrarlandi ve bu olcumlerin ortalama degerleri kaydedildi [ µl / (dk * cmH2O * cm2) ]. Sizinti deneylerinden elde edilen verilerin istatistiksel analizinde SPSS 20 programi kullanildi. Bulgular: Elde edilen veriler tek yonlu ANOVA ve post-hoc Tukey testleri kullanilarak analiz edildi. Gruplar arasinda istatistiksel fark bulundu. En az sizinti internal matrix in 1mm genislikte ortograt uygulanan grupta goruldu. En fazla sizinti ise1.4mm acikliga sahip dislerde retrograt uygulamada gozlendi. MTA’nin internal matrix ile birlikte kullanildigi gruplarda ve MTA’nin iki asamali yerlestirildigi gruplarda sizinti miktari MTA’nin tek seferde yerlestirildigi gruplardan daha azdir. Sonuc: Calismamizda uyguladigimiz ortograt prosedurde internal matrix ile birlikte MTA uygulandiginda apikal sizinti degerleri en azdir. Klinik olarak bu metodun tercih edildigi durumlarda, cerrahi uygulama sayilari azaltilabilir ve daha az invaziv uygulamalarla tedaviler yapilabilir. Anahtar Kelimeler : Immatur dis, Mikrosizinti, MTA, Sivi filtrasyon Evaluation Of Apical Leakage of Different Apical Diameter Teeth Used MTA Combined With Internal Matrix ABSTRACT Purpose: The aim of this study is to determine the effect of apical foramen diameter, and different application methods on the sealing ability of MTA using computerized fluid filtration device. Material and methods: 100 extracted human teeth were used for this study. In our study, apical foramen were prepared diameter of 1- 1.4 mm in width for to simulate the open apex with an immature teeth. MTA was mixed according to manufacturer's recommendations, and was placed into the root canals which have a different apical foramen diameter. by four different ways (retrograde way, two-phased ortograde way, and ortograde with or without internal matrix way). The teeth were prepared for the microleakage test with computerise fluid-filtration method. Measurements of fluid movement were made at intervals of 15-seconds and repeated 8 times and means of these measurements were recorded [ µl / (min * cmH 2 O * cm 2 ) ]. SPSS 20 software was used for statistical analysis of apical leakage of data. The data was analysed with one-way ANOVA and post-hoc Tukey tests. Results: The amount of leakage in groups the internal matrix used together with MTA and two stages arranged MTA are less than the group placed one step MTA. Conclusion: In our study, less apical leakage was determined in performed ortograde procedure with internal matrix. When this method is prefered clinically, the cases can be treated by less invasive application and reducing the number of surgery. Key words : fluid filtration, immature tooth, leakage, MTA.

The aim of this study was to compare femtosecond and Er:YAG laser systems with regard to enamel demineralization and bracket bond strength. Human-extracted premolars were randomized to three groups (n = 17) depending on the conditioning treatment used for the buccal surfaces: 37 % orthophosphoric acid, Er:YAG laser etching (MSP mode 120 mJ, 10 Hz, 1.2 W), and femtosecond laser etching (0.4 W, 800 nm, 90 fs/pulse, 1 kHz). Metal brackets were bonded with Transbond XT to the conditioned surfaces and light cured for 20 s. The samples were thermocycled (5000 cycles, 5-55 °C) and subjected to shear bond strength (SBS) testing using a universal testing machine. Failure types were analyzed under an optical stereomicroscope and SEM. The adhesive remnant index (ARI) was evaluated to assess residual adhesive on the enamel surface. The results revealed no significant differences in SBS between the Er:YAG laser (7.2 ± 3.3 MPa) and acid etching groups (7.3 ± 2.7 MPa; p

WOS: 000355962800003, PubMed: 25736260, To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p

Objective: To evaluate the marginal gap (MG) and absolute marginal discrepancy (MD) of full ceramic crowns with two finish line designs, shoulder and chamfer, using microcomputed tomography (micro-CT) before and after cementation. Materials and Methods: Sixty extracted human maxillary premolar teeth were divided into two groups based on the finish line design: Group I: 90° shoulder and Group II: 135° chamfer. The specimens were further grouped based on the type of full ceramic crown they received: Group A: Feldspathic Cerec inLab ceramic system, Group B: Cerec inLab aluminum oxide ceramic system and Group C: Lithium disilicate press ceramic system. Before cementation, five crowns from each group were scanned using micro-CT in two sections, sagittal and coronal, to determine the MG and MD values for four regions of the crown (sagittal buccal, sagittal lingual, coronal mesial and coronal distal). After cementation and thermal cycling, the scanning was repeated. Measurements were obtained from 10 points for each region, 80 points totally, to evaluate the MG and MD values. Files were processed using NRecon and CTAn software. Results were statistically analyzed using one- and two-way ANOVA and Tukey HSD tests (P = 0.05). Results: Full ceramic systems showed clinically acceptable marginal adaptation values. The Feldspathic Cerec inLab ceramic system generally presented the lowest variance, except in the MG values of the coronal mesial region. The MG and MD values of all ceramics increased significantly after cementation, except in the shoulder preparation design (sagittal buccal region) for MG and in the chamfer preparation design (sagittal lingual region) for MD values. Conclusions: Full-ceramic crowns showed clinically acceptable marginal adaptation values. The Feldspathic Cerec inLab ceramic system (Vitablocs Mark II) generally presented the lowest variance when compared with the other ceramics, except for the MG values on the mesial surface of the coronal section.

Auricular defects may result from congenital malformations, injuries from motor vehicle crashes, trauma, or tumor resections. An auricular prosthesis was fabricated for a patient who had an injury from a motor vehicle crash. Extraoral implants and bar-and-clip retention for the proper connection of the auricular prosthesis to implant were used. This prosthesis was acceptable to the patient because of excellent support, retentive abilities, and the patient's appearance.

Malignant tumors of the nose occasionally require rhinectomy. Facial defects can cause not only functional problems but also some serious physchological problems that could cause the individual to avoid social contact. In view of this, the first aim of maxillofacial rehabilitation should be solving esthetic problems. Implant retained prostheses can give good esthetic results. Predictable biomechanical retention of nasal prostheses can be achieved using osseointegrated implants and intra-oral and craniofacial implants have been used previously. These prostheses are tissue compatible, simple in design and easy to place, can be worn independently of spectacles and in the majority of cases can be provided soon after surgical excision. In this case report, a nasal prosthesis was fabricated for a patient who lost his nose due to the basal cell carcinoma. Extra-oral implants and magnet retention for the proper connection of the nasal prosthesis were used. This prosthesis was acceptable for the patient because of excellent support, retentive abilities and the patient's appearance.

Aim: The aim of this in vitro study was to compare the effect of three different post systems cemented with different systems on root fracture. Materials and Methods: Seventy-five extracted human maxillary canines with single and straight roots were used in this study. After the crowns were removed, root canals were prepared with Reciproc R50 files. Smear layer of roots were removed using 17% Ethylenediaminetetraacetic acid (EDTA) followed by 5.25% NaOCl and distilled water. All the root canals were filled with cold lateral compaction technique using AH Plus root canal sealer and gutta-percha. Samples were stored at 37°C and 100% humidity for 1 week and then post space was prepared using fiber post drills. The roots were then randomly divided into five groups according to the luting cements and post systems: negative control, positive control, glass fiber post [Unicore® (Ultradent, Salt Lake City UT, USA)] + composite core [Grandio SO (Voco GmbH, Cuxhave, Germany)], glass fiber post [Unicore® ] + Rebilda® post-core system, individual cast post core. A load was applied on the crowns of all teeth at 135° to their long axis until fracture occurred. Data were analyzed using one-way analysis of variance (ANOVA) and post-hoc Tukey tests. Results: There was statistically significant difference between the groups. According to the post-hoc Tukey test, cast post core (1949.35 + 316 N) showed statistically significantly higher fracture resistance than all the groups except Unicore® + Rebilda® post-core systems (1722.48 + 144.0 N). Conclusion: Fiber post core system which is an alternative to cast post core systems increased the fracture resistance of the canines with root canal treatment. While irrepairable catastrophic fractures might be seen in cast post-core systems, separations between composite resin core, and root interfaces might be seen in teeth restored with fiber post-core systems.