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Introduction: Oral hygiene instruction performed by different personnel in various manners is a necessity for prevention and treatment of dental caries and periodontal diseases. Since dentists are the majority of personnel who deliver oral hygiene instruction to patients, this study was designed to evaluate dentists' attitude towards oral hygiene instruction in their private office in Yazd. Methods: A questionnaire including demographic information and various oral hygiene instruction methods was prepared and delivered to 151 dentists who were working in their private office in Yazd. Data were analyzed with SPSS 16 software and Fisher exact test was performed. Results: Results revealed that 95.3% of dentists gave oral hygiene instruction to their patients. This training included dental flossing techniques followed by tooth brushing. Therefore, using an oral model was the most common way of patient's instruction. Conclusion: Results obtained from this study showed that the majority of dentists in Yazd gave oral hygiene instruction to their patients through various methods. [ABSTRACT FROM AUTHOR] more...