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1. First round of external quality assessment of dengue diagnostics in the WHO Western Pacific Region, 2013.

Author

Pok KY, Squires RC, Tan LK, Takasaki T, Abubakar S, Hasebe F, Partridge J, Lee CK, Lo J, Aaskov J, Ng LC, and Konings F

Subjects
Antibodies, Viral analysis, Antibodies, Viral blood, Antigens, Viral analysis, Asia, Southeastern, Australasia, Dengue virology, Dengue Virus genetics, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin M analysis, Pacific Islands, Quality Assurance, Health Care, RNA, Viral analysis, World Health Organization, Dengue diagnosis, Dengue Virus isolation & purification, Reverse Transcriptase Polymerase Chain Reaction standards, Serologic Tests standards
Abstract

Objective: Accurate laboratory testing is a critical component of dengue surveillance and control. The objective of this programme was to assess dengue diagnostic proficiency among national-level public health laboratories in the World Health Organization (WHO) Western Pacific Region., Methods: Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted., Results: All 16 laboratories testing Module A performed reverse transcriptase polymerase chain reaction (RT-PCR) for both RNA and serotype detection. Of these, 15 had correct results for RNA detection and all 16 correctly serotyped the viruses. All nine laboratories performing NS1 antigen detection obtained the correct results. Sixteen of the 18 laboratories using IgM assays in Module B obtained the correct results as did the 13 laboratories that performed IgG assays. Detection of ongoing/recent dengue virus infection by both molecular (RT-PCR) and serological methods (IgM) was available in 15/19 participating laboratories., Discussion: This first round of external quality assessment of dengue diagnostics was successfully conducted in national-level public health laboratories in the WHO Western Pacific Region, revealing good proficiency in both molecular and serological testing. Further comprehensive diagnostic testing for dengue virus and other priority pathogens in the Region will be assessed during future rounds.

Published
2015
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2. Recent Epidemiological Trends of Dengue in the French Territories of the Americas (2000–2012): A Systematic Literature Review

Author

Claude Flamand, Anne-Frieda Taurel, Philippe Quénel, Maïna L’Azou, Sanofi Pasteur [Lyon, France], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), and Sanofi Pasteur sponsored this survey and analysis. The authors (including members of Sanofi Pasteur) were responsible for the conception of the literature analysis, development of the protocol, data collection, analysis and interpretation of data, provision of critical comments, writing the paper and approving the final version to be published.

Subjects
lcsh:Arctic medicine. Tropical medicine, MESH: West Indies, lcsh:RC955-962, 030231 tropical medicine, MESH: Dengue, Dengue virus, medicine.disease_cause, MESH: Dengue Virus, Dengue fever, MESH: Sex Ratio, 03 medical and health sciences, 0302 clinical medicine, Environmental health, MESH: Severity of Illness Index, MESH: French Guiana, Medicine, Seroprevalence, 030212 general & internal medicine, MESH: Incidence, MESH: Disease Outbreaks, MESH: Age Distribution, Dengue vaccine, MESH: Epidemics, Disease surveillance, MESH: Humans, MESH: Seroepidemiologic Studies, business.industry, lcsh:Public aspects of medicine, MESH: Sex Distribution, Public Health, Environmental and Occupational Health, Outbreak, lcsh:RA1-1270, MESH: Serogroup, medicine.disease, 3. Good health, Infectious Diseases, 2009 Bolivian dengue fever epidemic, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, Martinique
Abstract

International audience; Dengue is a public health concern across the globe, and an escalating problem in the Americas. As part of a wider programme (covering Latin America and South East Asia) to characterize the epidemiology of dengue in dengue endemic areas, we undertook a systematic literature review to assess epidemiological trends (incidence, timing and duration of outbreaks/epidemics, age and sex distribution, serotype distribution, seroprevalence and disease severity) for dengue across the French Territories of the Americas (FTA), in French Guiana, Guadeloupe, Martinique, Saint Martin and Saint Barthélemy between 2000 and 2012 (CRD42012002341: http://www.crd.york.ac.uk/prospero/display_record.asp?ID=CRD42012002341). Of 413 relevant data sources identified, 45 were eligible for inclusion. A large proportion of the available data were from national surveillance reports, and 12 publications were from peer-reviewed journals. During the review period, 3-5 epidemics were identified in each of the island territories and French Guiana, and epidemics were often associated with a shift in the predominant circulating dengue virus serotype. Substantial gaps in epidemiological knowledge were identified. In particular, information regarding dengue virus genotype distribution, seroprevalence and age distribution of dengue were lacking. Additionally, much of the available data were from epidemic years; data from inter-epidemic periods were sparse. Nevertheless, the available epidemiological data showed that dengue is endemic across the FTA and suggest an evolution towards hyperendemicity, highlighting the need to continue the efforts with the existing surveillance programmes to assist in planning an effective vaccination programme once a dengue vaccine is deployed.

Published
2014