Search

Made available in DSpace on 2018-11-26T15:37:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 Pronex INCT-Dengue Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in Sao Jose do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nestedPCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RTPCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV. (C) 2016 Elsevier B.V. All rights reserved. Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP, Brazil Univ Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil Dept Vigilancia Saude, Prefeitura Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP, Brazil Univ Fed Minas Gerais, Dept Microbiol, Belo Horizonte, MG, Brazil Univ Fed Mato Grosso, Inst Ciencias Saude, Sinop, MT, Brazil Univ Texas Med Branch, Ctr Biodefense & Emerging Infect Dis, Ctr Trop Dis, Inst Human Infect & Immun, Galveston, TX 77555 USA Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA Univ Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP, Brazil

Dengue is a serious public health problem in numerous countries. The ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.The present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio(®) Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.In samples collected in a non-epidemic period we found a 100% agreement of ELISA and RT-PCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p0.05) in dengue negative samples.Due to false negative results for DENV-4, the sole use of the Panbio(®) Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated.