1. Functional and transcriptional heterogeneity within the massively expanding HLADR + CD38 + CD8 T cell population in acute febrile dengue patients.
- Author
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Singh P, Bajpai P, Maheshwari D, Chawla YM, Saini K, Reddy ES, Gottimukkala K, Nayak K, Gunisetty S, Aggarwal C, Jain S, Verma C, Singla P, Soneja M, Wig N, Murali-Krishna K, and Chandele A
- Subjects
- Humans, Cytokines, Interferon-gamma genetics, Fever virology, T-Lymphocyte Subsets immunology, CD8-Positive T-Lymphocytes immunology, Dengue genetics, Dengue immunology, Dengue pathology
- Abstract
Importance: CD8 T cells play a crucial role in protecting against intracellular pathogens such as viruses by eliminating infected cells and releasing anti-viral cytokines such as interferon gamma (IFNγ). Consequently, there is significant interest in comprehensively characterizing CD8 T cell responses in acute dengue febrile patients. Previous studies, including our own, have demonstrated that a discrete population of CD8 T cells with HLADR
+ CD38+ phenotype undergoes massive expansion during the acute febrile phase of natural dengue virus infection. Although about a third of these massively expanding HLADR+ CD38+ CD8 T cells were also CD69high when examined ex vivo , only a small fraction of them produced IFNγ upon in vitro peptide stimulation. Therefore, to better understand such functional diversity of CD8 T cells responding to dengue virus infection, it is important to know the cytokines/chemokines expressed by these peptide-stimulated HLADR+ CD38+ CD8 T cells and the transcriptional profiles that distinguish the CD69+ IFNγ+ , CD69+ IFNγ- , and CD69- IFNγ- subsets., Competing Interests: The authors declare no conflict of interest.- Published
- 2023
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